Effects of Serum Starvation on Radiosensitivity, Proliferation and Apoptosis in Four Human Tumor Cell Lines with Different p53 Status

Oya, Natsuo; Zölzer, Friedo; Werner, Frank; Streffer, C.

doi:10.1007/s00066-003-0973-8

Cited by 24 publications

(24 citation statements)

References 29 publications

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“…The culturing of whole blood [22] or all mononuclear cells [1] together and using flow cytometry methods by labeling the cells may have an advantage over the cell separation and culturing relatively small numbers of cells of a homogeneous subpopulation. According to our experience, culturing cells in conical tubes with a high cell density and sufficient nutrients can achieve a stress-free [27] cultivation of the isolated CD3 + cells.…”

Section: Abbildung 3 Altersabhängigkeit Der Apoptoseraten In CD 3+ -mentioning

confidence: 99%

Individual Radiosensitivity Does not Correlate with Radiation-Induced Apoptosis in Lymphoblastoid Cell Lines or CD3+ Lymphocytes

et al. 2005

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Only a subgroup of hypersensitive patients may be identified by reduction of radiation-induced apoptotic rate. It is concluded that the hypothesis according to which hypersensitive cells have reduced levels of apoptosis is only conditionally true. The authors suggest that this assay can be used in combination with additional tests evaluating DNA double-strand break repair, cell-cycle control and chromosomal aberrations for the evaluation for individual hypersensitivity.

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Section: Abbildung 3 Altersabhängigkeit Der Apoptoseraten In CD 3+ -mentioning

confidence: 99%

Individual Radiosensitivity Does not Correlate with Radiation-Induced Apoptosis in Lymphoblastoid Cell Lines or CD3+ Lymphocytes

et al. 2005

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“…Although serum starvation can be effective, there are several drawbacks to the technique. Serum starvation can have many negative consequences including induction of DNA fragmentation (Niemann et al, 2000), decreased mitochondrial viability (Takeda et al, 2002), alterations or disruption of the cell cycle following serum starvation (Gray and Darzynkiewicz, 1987) and the induction of apoptosis or death (Magnani and Bettini, 2000;Oya et al, 2003;Tan et al, 2003). In addition, some transformed and primary cell lines, including primary rabbit corneal epithelial cells are resistant to the effects of serum starvation and will not synchronize with this method (Knehr et al, 1995;Cooper, 1998;Anderson and SundarRaj, 2001;Katska et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Cell sorting but not serum starvation is effective for SV40 human corneal epithelial cell cycle synchronization

Liliensiek¹,

Schell²,

Howard³

et al. 2006

Experimental Eye Research

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SV40 human corneal epithelial cell (HCEC) populations are readily used as a substitute for primary corneal epithelial cells that are difficult to maintain in vitro. To initiate cell-cycle experiments with the SV40-HCEC cells, two separate methods of cell synchronization were compared including serum starvation and sterile cell sorting. We hypothesized that SV40 cells are synchronized at higher efficiencies into each cell cycle phase (G1, S, G2M) when cell sorting is performed when compared to alternative methods of synchronization. SV40 cells were synchronized by deprivation of serum over 96 h or labeled with Höechst 33342 dye and sorted based on DNA content. Cells were synchronized using both methods and harvested at time points up to 72 h after release. To define more precisely the nature of sorted fractions, cells were pulsed with BrdU prior to sorting. SV40-HCEC cells exhibit a well-defined cell cycle profile. Serum deprivation up to 96 h was ineffective for cell synchronization of SV40-HCECs. In comparison, we achieved efficient synchronization of the SV40-HCECs with sterile cell sorting. SV40-HCEC cells gated into G 1 , S and G 2 M were synchronized up to 85% following the sort and maintained synchronization up to 24 h. Our findings indicate that serum starvation is not effective for synchronization of the SV40-HCEC cell line. We present a more effective approach, the use of cell sorting for cell synchronization of the SV40-HCEC cells.

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“…Two-parameter flow cytometric analysis (FCMA) with a DNA-dye-exclusion annexin-V-binding assay was performed to detect apoptosis [21]. The experiments were carried out using Annexin V-FITC Apoptosis Detection Kit (Calbiochem, Darmstadt) according to the attached protocol of the providing company with slight modification.…”

Section: Analysis Of Apoptosismentioning

confidence: 99%

Similar Extent of Apoptosis Induction at Doses of X-Rays and Neutrons Isoeffective for Cell Inactivation

et al. 2008

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Effects of Serum Starvation on Radiosensitivity, Proliferation and Apoptosis in Four Human Tumor Cell Lines with Different p53 Status

Cited by 24 publications

References 29 publications

Individual Radiosensitivity Does not Correlate with Radiation-Induced Apoptosis in Lymphoblastoid Cell Lines or CD3+ Lymphocytes

Individual Radiosensitivity Does not Correlate with Radiation-Induced Apoptosis in Lymphoblastoid Cell Lines or CD3+ Lymphocytes

Cell sorting but not serum starvation is effective for SV40 human corneal epithelial cell cycle synchronization

Similar Extent of Apoptosis Induction at Doses of X-Rays and Neutrons Isoeffective for Cell Inactivation

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