Effects of sesamin on primary human synovial fibroblasts and SW982 cell line induced by tumor necrosis factor-alpha as a synovitis-like model

Abstract

BackgroundRheumatoid arthritis (RA) is an autoimmune disease that causes chronic synovitis, cartilage degradation and bone deformities. Synovitis is the term for inflammation of the synovial membrane, an early stage of RA. The pathogenesis of the disease occurs through cytokine induction. The major cytokine that increases the severity of RA is TNF-α. Thus, inhibition of the TNF-α cascade is an effective way to diminish the progression of the disease. We are interested in investigating the difference between primary human synovial fibroblast (hSF) cells and SW982 as synovitis models induced by TNF-α and in monitoring their responses to sesamin as an anti-inflammatory phytochemical.MethodThe designed experiments were performed in hSF cells or the SW982 cell line treated with 10 ng/ml TNF-α with or without 0.25, 0.5 or 1 μM sesamin. Subsequently, pro-inflammatory cytokine genes and proteins were measured in parallel with a study of associated signalling transduction involved in inflammatory processes, including NF-κB and MAPK pathways.ResultsThe results demonstrated that although hSF and SW982 cells responded to TNF-α induction in the same fashion, they reacted at different levels. TNF-α could induce IL-6, IL-8 and IL-1β in both cell types, but the levels in SW982 cells were much higher than in hSF cells. This characteristic was due to the different induction of MAPKs in each cell type. Both cell types reacted to sesamin in almost the same fashion. However, hSF cells were more sensitive to sesamin than SW982 cells in terms of the anti-RA effect.ConclusionsThe responses of TNF-α-induced hSF and SW982 were different at the signal transduction level. However, the two cell types showed almost the same reaction to sesamin treatment in terms of the end point of the response.Electronic supplementary materialThe online version of this article (10.1186/s12906-017-2035-2) contains supplementary material, which is available to authorized users.

γ-Mangostin isolated from Garcinia mangostana L. suppresses inflammation and alleviates symptoms of osteoarthritis via modulating miR-124-3p/IL-6/NF-κB signaling

“…Although the mobility of OA patients appeared to be very limited and painful due to inflammation [32]. We confirmed that MS and γ-MS treatment improves the mobility significantly by subjecting treated and nontreated mice to an open field test to record their movement.…”

Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation

“…The significant inhibition of SGE on synovial hyperplasia and inflammation on rats inspired us a further investigation into the therapeutic effects and mechanisms on the human fibroblast-like synoviocytes. The best-known cell model used to study synovitis in RA is the human synovial sarcoma cell line (SW982) [20]. Hence, we validated the effects and mechanisms of SGE on human SW982 synovial cell.…”

Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation

“…The significant inhibition of SGE on synovial hyperplasia and inflammation on rats inspired us a further investigation into the therapeutic effects and mechanisms on the human fibroblast-like synoviocytes. The best-known cell model used to study synovitis in RA is the human synovial sarcoma cell line (SW982) [19]. Hence, we validated the effects and mechanisms of SGE on human SW982 synovial cell.…”