Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture

Pothacharoen, Peraphan; Najarus, Sumet; Settakorn, Jongkolnee; Mizumoto, Shuji; Sugahara, Kazuyuki; Kongtawelert, Prachya

doi:10.1007/s10719-013-9514-6

Cited by 15 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest CSPGs gene expression in normal HAC was on day 14, while in IL-1β induced HAC, the highest expression of CHSY1 and ChPF was on day 7, while for ACAN, XT-1 and XT-2 it was on day 14 which differs from a previous study [9]. The difference of CSPGs expression pattern in IL-1β-induced HAC may have been due to chondrocyte compensatory mechanisms which counter IL-1β action [23].…”

Section: Discussioncontrasting

confidence: 99%

“…Sesamin has previously demonstrated regulation of the chondrocyte catabolic process via suppression of expression of MMPs through the inhibition of IL-1β signaling cascades [4]. It has also shown induction on CSPGs proteoglycan synthesis via enhancement of chondrocyte CSPGs synthetic genes [9]. …”

Section: Discussionmentioning

confidence: 99%

“…The GAGs release from the HAC pellets and GAGs accumulation were measured in culture media and papain digested pellets by DMMB assay [9]. Matrix GAGs accumulation in pellet sections was investigated using safranin O staining.…”

Section: Methodsmentioning

confidence: 99%

“…Reported pharmacological properties of sasamin include anti-inflammation [4], anti-oxidant [5], anti-hypertensive, inducing apoptosis in cancer cells [6], lowering blood cholesterol, improving fatty acid metabolism [7] and neuroprotective effect against hypoxia [8]. A recent study described the chondroprotective effect of sesamin on normal human articular chondrocytes (HAC) works by promoting matrix sulfated glycosaminoglycans (GAGs) content and upregulation of the chondroitin sulfate proteoglycan (CSPGs) synthesis genes: ACAN, XT-1, XT-2, CHSY1 and ChPF resulting in a protective affect against OA [9]. In this study we evaluated the effect of sesamin on pathological HAC in pellet culture in which the inflammation process in OA pathogenesis had been induced by IL-1β.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes

Srisuthtayanont

Pruksakorn

Kongtawelert

et al. 2017

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

BackgroundNumerous studies have reported on the health benefits of sesamin, a major lignin found in sesame (S. indicum) seeds. Recently, sesamin was shown to have the ability to promote chondroitin sulfate proteoglycan synthesis in normal human chondrocytes. This study assesses the anti-inflammatory effect of sesamin on proteoglycans production in 3D chondrocyte cultures.MethodsTo evaluate the effects of sesamin on IL-1β-treated human articular chondrocytes (HAC) pellets, the pellets were pre-treated with IL-1β then cultured in the presence of various concentrations of sesamin for 21 days. During that period, the expression of IL-1β, glycosaminoglycans (GAGs) content and Chondroitin sulfate proteoglycans (CSPGs) synthesis genes (ACAN, XT-1, XT-2, CHSY1 and ChPF) was measured. The GAGs accumulation in the extracellular matrix was determined on day 21 by histological analysis.ResultsThere was clear evidence that sesamin upregulated expression of all the CSPGs synthesis genes, in contrast to the down-regulation of IL-1β expression both in genes and in protein levels. The level of release and matrix accumulation of GAGs in IL-1β pre-treated HAC pellets in the presence of sesamin was recovered. These results correlate with the histological examination which showed that sesamin enhanced matrix CSPGs accumulation.ConclusionsSesamin enhances CSPGs synthesis, suppresses IL-1β expression and ameliorates IL-1β induced inflammation in human chondrocytes. Sesamin could have therapeutic benefits for treating inflammation in osteoarthritis.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes

Srisuthtayanont

Pruksakorn

Kongtawelert

et al. 2017

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…[19] MSC and chondrocytes grown in 3D cell aggregates are capable to produce a cartilaginous matrix, similar to native tissue, [17,20] and provide a simple tool to screen new drugs for articular disorders, like OA. [21] Bhumiratana et al took these simple 3D models a step further by fusing several cell aggregates (condensed mesenchymal cell bodies (CMBs)), to form a cartilage-like layer in an in vitro model using bone decellularized ECM, and to fill a cartilage defect in an ex vivo cartilage explant model. CMBs formed cartilage like-tissue, with glycosaminoglycans (GAGs) and type II collagen (COL2) production, and integrated in the decellularized bone or native cartilage explants, indicating that this methodology has the potential to create implantable solutions to repair cartilage defects.…”

Section: From Basic To Advanced Cartilage Models: New Strategies To Dmentioning

confidence: 99%

Articular Repair/Regeneration in Healthy and Inflammatory Conditions: From Advanced In Vitro to In Vivo Models

Teixeira

Pereira

Almeida

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

The burden of chronic inflammatory diseases of joints and the spine is increasing with population ageing and unhealthy lifestyles. Articular cartilage and intervertebral discs (IVDs) are avascular and aneural tissues with abundant extracellular matrix, low cell density, and reduced regenerative capacity after damage or degeneration. The most advanced in vitro and ex vivo cell-/tissue-/biomaterial-based models and technologies to improve physiological mimicry are discussed, focusing on the impact of inflammation on articular repair/regeneration. In addition, in vivo models, developed to study cartilage and IVD repair/regeneration are addressed. While animal models continue to provide crucial mechanistic and preclinical data, more advanced and robust in vitro/ex vivo cartilage and IVD models, with appropriate extracellular matrix cues and allowing for cellular crosstalk, have seen an exponential growth in the last decade. Due to the complexity of articular microenvironments, adequate in vitro/ex vivo/in vivo models are essential to study the molecular mechanisms underlying articular diseases and develop new therapies for repair/regeneration.

show abstract

Functional foods in improving bone health during aging

Charoensin

Pothacharoen

Wanachewin

et al. 2023

Plant Bioactives as Natural Panacea Against Age-Induced Diseases

View full text Add to dashboard Cite

Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture

Cited by 15 publications

References 40 publications

Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes

Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes

Articular Repair/Regeneration in Healthy and Inflammatory Conditions: From Advanced In Vitro to In Vivo Models

Functional foods in improving bone health during aging

Contact Info

Product

Resources

About