Effects of several storage media on viability and proliferation capacity of periodontal ligament cells

Souza, Beatriz Dulcinéia Mendes de; Garcia, Lucas da Fonseca Roberti; Bortoluzzi, Eduardo Antunes; Felippe, Wilson Tadeu; Felippe, Mara Cristina Santos

doi:10.1007/s40368-019-00450-8

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…Avulsion or exarticulation has been regarded to be one of the most serious types of traumatic dental injuries ( 42 ). Although various storage mediums such as milk, HBSS, and coconut water have been used to store avulsed teeth for the purpose of preserving the viability of PDL cells prior to replantation ( 13 ), and a systematic review and meta-analysis has also been conducted to investigate the comparative efficacy between the various storage medium and milk ( 7 ), no definitive conclusion about which storage media should be the optimal option for persevering viability of PDL cells prior to replantation has been generated currently.…”

Section: Discussionmentioning

confidence: 99%

Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

Zhang

Cheng

et al. 2021

Front. Med.

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Many storage mediums are available for the storage of avulsed teeth to preserve the viability of periodontal ligament (PDL) cells before replantation; however, it is unclear which medium is the optimal option. We performed this network meta-analysis to answer this question. A comprehensive search was conducted in PubMed, EMBASE, and the Cochrane library to capture eligible studies investigating the comparative efficacy of Hank's balanced salt solution (HBSS), aloe vera gel (AVG), oral rehydration solution (ORS), coconut water, egg white, green tea, propolis, saline, milk, and water. Statistical analysis was conducted using Review Manager v5.3 and ADDIS v1.16.8. In total, 20 RCTs involving 31 reports were included finally. Direct meta-analysis suggested that HBSS was superior to ORS, milk, saline, and water, ORS was superior to milk but inferior to coconut water and propolis, egg white was superior to milk but inferior to AVG and propolis, propolis was superior to AVG, milk, and saline, and coconut water and water was inferior to saline and milk, respectively. Network meta-analysis suggested that AVG was inferior to the other nine mediums, and propolis was superior to HBSS (SMD, −5260.24; 95% CrI, −10447.39 to −70.37) and milk (SMD, −5461.11; 95% CrI, −10574.99 to −328.51). Moreover, ranking probabilities indicated the highest probability for propolis, followed by saline, ORS, HBSS, milk, egg white, water, green tea, and AVG successively. Propolis may be the optimal media for storing avulsed teeth before replantation. However, given the availability of propolis and HBSS and the hypotonic properties of saline, ORS or milk should also be preferentially selected.

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Section: Discussionmentioning

confidence: 99%

Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

Zhang

Cheng

et al. 2021

Front. Med.

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“…Hence, the bone tissue may be isolated from the tooth and decreases the chances of ankylosis [30,31]. However, it should be noted that few reports argued that replanted teeth stored in milk were subject to ankylosis [32][33][34].…”

Section: Milkmentioning

confidence: 99%

“…HBSS is commercially available as "Save-A-Tooth." However, it is not found at most of the accident sites; this makes it an impractical storage medium [20,29,31,32,34].…”

Section: Hbss (Hanks' Balanced Salt Solution)mentioning

confidence: 99%

Perspective Chapter: Teeth Avulsion

Eltahir¹,

Ibrahim²,

Alharbi³

2023

Dentistry

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Tooth avulsion refers to total displacement of the tooth out of its alveolar socket. This chapter discusses the causes of avulsion for both primary and permanent dentition in addition to the prevalence of avulsion comparing it between before and during COVID-19 era. Successful outcomes depend on procedure conduction with immediate replantation of the avulsed tooth and good handling of the tooth keeping the tooth in suitable available storage media till a professional service is obtainable. Management of this type of dental trauma necessitates awareness and collaboration between the lay personal at the site of injury in addition to the important role of different dental professional. After replantation of the avulsed tooth, it should be kept immobile with preservation of the functional (physiological) mobility by application of suitable splint. There are adjunctive therapies including antibiotics, analgesics, mouth washes, and tetanus vaccines. Endodontic therapy should be initiated within 7–10 days from the time of avulsion, where the outcomes generally might be favorable or unfavorable. Newly emergent teledentistry played important role in the treatment of tooth avulsion, especially during COVID-19 period.

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“…1 To date, extensive investigations have been reported as to which storage medium potentially holds a favourable property in promoting survival of PDL cells on the tooth roots exposed due to a traumatic injury. [2][3][4][5][6] Considering the active chemical components, propolis extract contains flavonoids, phenolic acid and esters, aromatic compounds, and caffeic acid phenylethyl ester that possess a wide range of biological activities, including anti-oxidant, anti-bacterial, anti-viral, anti-fungal, anti-inflammatory and anti-tumor properties. 7,8 Therefore, propolis might exert a favourable outcome when used as a storage medium for avulsed teeth.…”

Section: Introductionmentioning

confidence: 99%

Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract

Bunwanna

Damrongrungruang

Puasiri

et al. 2020

Dental Traumatology

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Background/Aim: Success of tooth replantation depends on the quality and quantity of periodontal ligament (PDL) cells. The aims of this study were to evaluate Thai propolis extract as a storage medium for maintaining PDL cell viability and preserving gene expressions in PDL tissues. Materials and Methods: PDL cells from human premolars were tested for cytotoxicity of the extract by PrestoBlue assay to determine a non-toxic concentration. Subsequently, 96 freshly extracted premolars were allocated into different treatment groups. Control groups were freshly extracted premolars or they had been stored dry for 12 hours. Experimental avulsed teeth were created by leaving them air-dried for 30 minutes immediately after extraction, then they were immersed in Thai propolis extract, HBSS or milk for 3, 6 and 12 hours. After tooth storage, the remaining PDL cells were determined for their cell viability. RNA isolated from PDL tissues of three premolars treated similarly was analysed for periostin and S100A4 expressions using RT-qPCR. Results: Thai propolis extract at 0.625 mg mL −1 promoted the greatest PDL cell viability. Tooth storage in 0.625 mg mL −1 Thai propolis extract, HBSS or milk showed no difference in maintaining cell viability. Periostin mRNA level was preserved by Thai propolis extract. Expression of S100A4 mRNA in PDL tissues stored in all tested media was dampened. Conclusions: PDL cells from mock avulsed teeth stored in 0.625 mg mL −1 Thai propolis extract for 3, 6 and 12 hours remained viable and the expression of periostin was preserved. This study suggests this extract as an alternative for a tooth storage medium for up to 12 hours. However, transporting an avulsed tooth in a storage medium for extended extra-oral time might affect the PDL cell phenotypes.

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Effects of several storage media on viability and proliferation capacity of periodontal ligament cells

Cited by 11 publications

References 30 publications

Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

Perspective Chapter: Teeth Avulsion

Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract

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