Effects of sex and age on the osmotic stability of Sahel goat erythrocytes

Igbokwe, Nanacha Afifi; Ojo, Nicholas Adetayo; Igbokwe, I.O.

doi:10.1007/s00580-015-2130-z

Cited by 12 publications

(10 citation statements)

References 38 publications

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“…Immune-mediated hemolytic anaemia has been reported in bovine anaplasmosis and theileriosis [23]. Osmotic erythrocyte fragility test is widely used as an aid to diagnosis of certain haematological disorders such as intravascular haemolytic anaemia, autoimmune diseases, spherocytosis, elliptocytosis and stomatocytosis [37]. Blood parasites cause alteration in the red cell membrane of affected animals thereby making erythrocytes highly susceptible to lysis when subjected to hypoosmotic stress [37,38].…”

Section: Bovine Hemoplasmosis Is Caused By the Hemotropic Mycoplasmasmentioning

confidence: 99%

First report of bovine anaemia associated Theileria sinensis infection and phylogenetic analyses of partial gene sequences of Theileria and Anaplasma species detected in naturally infected Malaysian cattle

Agina¹,

Shaari²,

Isa³

et al. 2020

Preprint

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Background: Serious disease outbreaks in cattle are usually associated with blood pathogens. This study aims to detect blood pathogens namely Trypanosoma evansi, Theileria, Anaplasma and Mycoplasma species, and studied their phylogenetic relationships, haemato-biochemical abnormalities and erythrocyte osmotic fragility (EOF) in Malaysian cattle. Methods: Molecular analysis was achieved by PCR amplification and sequencing of PCR amplicons of 18SrRNA gene of Theileria species, 16SrRNA genes of Anaplasma and Mycoplasma species, 16SrRNA gene of Candidatus Mycoplasma haemobos, MPSP gene of T. orientalis and RoTaT1.2 VSG gene of Trypanosoma evansi, in sixty-one (61) Kedah-Kelantan X Brahman cattle from Pahang, Malaysia. Haemato-biochemical analyses were performed using automated analysers while EOF was determined with the aid of saline solutions. Results: PCR amplification produced the expected fragment sizes for MPSP gene of T. orientalis, msp4 gene of Anaplasma marginale, 16S rRNA gene of C. M. haemobos, RoTaT1.2VSG gene of T. evansi. Nucleotide blast demonstrated that sequences of the PCR amplicons showed a high degree of molecular similarity in comparison with reference sequences. Analysis of 18SrRNA gene sequences of Theileria species and 16S rRNA gene sequences of Anaplasma species revealed Theileria sinensis and Anaplasma platys as additional species detected in these cattle. Theileria species was the most detected blood pathogen in the sampled cattle. The blood picture of all cattle group revealed poikilocytosis, anisocytosis, rouleaux formation and degenerative left shift. Erythrocyte fragility values of all the cattle groups were above the reference range. Anaemia of the macrocytic normochromic type was observed in the Trypanosoma evansi; and Anaplasma platys + Theileria sinensis double species co-infected cattle. Normocytic normochromic anaemia was observed in the T. sinensis infected cattle group. Significant (p<0.05) increases in serum liver and kidney parameters, total protein, globulin, total and unconjugated bilirubin and decreased albumin was observed in the Trypanosoma evansi infected cattle.Conclusion: We present the first report of anaemia associated with Theileria sinensis infection in Malaysian cattle. Because of the high occurrence of bovine theileriosis and detection of Anaplasma platys, there is an urgent need for appropriate preventive and control measures, as Theileria species and A. platys are of great economic and zoonotic importance respectively.

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Section: Bovine Hemoplasmosis Is Caused By the Hemotropic Mycoplasmasmentioning

confidence: 99%

First report of bovine anaemia associated Theileria sinensis infection and phylogenetic analyses of partial gene sequences of Theileria and Anaplasma species detected in naturally infected Malaysian cattle

Agina¹,

Shaari²,

Isa³

et al. 2020

Preprint

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“…Additionally, the EOF curves exhibited a rightward shift, and this further confirmed the increased instability of the red blood cell membrane induced by blood pathogens [ 84 ]. In general, blood pathogens induce structural, biochemical, and functional damage to the erythrocyte membrane (degradation of the membrane polyunsaturated fatty acids) and make them vulnerable to reactive oxygen species and susceptible to hemolysis [ 8 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Immune-mediated haemolytic anaemia has been reported in bovine anaplasmosis and theileriosis [ 24 ]. Osmotic erythrocyte fragility test is widely used as an aid to diagnosis of certain haematological disorders such as intravascular haemolytic anaemia, autoimmune diseases, spherocytosis, elliptocytosis and stomatocytosis [ 37 , 38 ]. Evaluation of the membrane stability of erythrocytes under hypoosmotic stress is performed by lysis of erythrocytes in decreasing concentrations of hypotonic buffered saline solutions [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

“…Erythrocyte osmotic fragility test is widely used as an aid to diagnosis of certain haematological disorders that involves alteration in erythrocyte membrane such as intravascular haemolytic anaemia, autoimmune diseases, hereditary spherocytosis, elliptocytosis and stomatocytosis [ 40 ]. Abnormalities in erythrocyte membrane also occur in bovine blood pathogen diseases such as babesiosis, trypanosomosis and anaplasmosis [ 8 , 37 , 41 , 42 ]. Other factors such as nutritional status, hormonal imbalance, drugs and chemical intoxications, age, transportation stress could also increase the fragility of erythrocytes [ 43 – 45 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle

et al. 2021

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Background Serious disease outbreaks in cattle are usually associated with blood pathogens. This study aims to detect blood pathogens namely Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos and Trypanosoma evansi, and determine their phylogenetic relationships and haemato-biochemical abnormalities in naturally infected cattle. Methods Molecular analysis was achieved by PCR amplification and sequencing of PCR amplicons of 18SrRNA gene of Theileria species, 16SrRNA genes of Anaplasma and Mycoplasma species, MPSP genes of T. orientalis and T. sinensis, MSP4 gene of A. marginale, 16SrRNA gene of Candidatus Mycoplasma haemobos, and RoTat1.2 VSG gene of Trypanosoma evansi, in sixty-one (61) clinically ill Kedah-Kelantan x Brahman cattle in Pahang, Malaysia. Results A total of 44 (72.13%) cattle were infected with more than one blood pathogen. Theileria species was the blood pathogen with the highest molecular detection rate (72.13, 95% CI 59.83–81.81%). Nucleotide blast analyses of all sequences demonstrated high degree of molecular similarity (98–100%) in comparison with their respective reference sequences. Analysis of 18SrRNA gene sequences of Theileria species and 16SrRNA gene sequences of Anaplasma species revealed Theileria sinensis and Anaplasma platys respectively as additional species detected in these cattle. MPSP-PCR analysis was conducted for further confirmation of T. sinensis. The blood picture of eight infected cattle groups revealed poikilocytosis, anisocytosis, rouleaux formation and degenerative left shift. High mean erythrocyte fragility values were common in infected cattle groups. Anaemia of the macrocytic normochromic type and spherocytes were observed in the T. evansi and Anaplasma platys + Theileria sinensis double species co-infected cattle group. Normocytic normochromic anaemia was observed in the T. sinensis infected cattle group. Significant (p < 0.05) increases in serum liver and kidney parameters, total protein, globulin, total and unconjugated bilirubin and decreased albumin values were observed in the T. evansi infected cattle when compared to clinically healthy cattle. Conclusion We present the first evidence of Theileria sinensis-associated bovine anaemia (TSABA) in Malaysian cattle. Because of the high occurrence of bovine theileriosis and detection of A. platys, there is an urgent need for appropriate preventive and control measures against these blood pathogens.

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“…Erythrocyte osmotic fragility has been used as an indicator of oxidative stress in animals (13). The relationship between aging and osmotic fragility differs (14). In some studies, performed on elderly human and bovine erythrocytes, it has been shown that the osmotic fragility decreases as the erythrocyte in the circulation ages (15).…”

Section: Introductionmentioning

confidence: 99%

Kangal Çoban Köpeklerinde Yaş ve Cinsiyetin Bazı Hematolojik Parametreler ve Eritrosit Ozmotik Kırılganlık Üzerine Etkisi

Koçkaya¹,

Ekici²,

GENÇ³

et al. 2021

Atatürk Üniversitesi Veteriner Bilimleri Dergisi

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This study was aimed to determine the effect of age and gender on physiological variations in some hematological parameters and erythrocyte osmotic fragility in Kangal Shepherd dogs. A total of 32 clinically healthy Kangal Shepherd dogs were taken into the study and divided into 4 groups in equal numbers (Adult male = 8, Adult female = 8, Senior male = 8, Senior female = 8). Blood samples from the cephalic vein were taken into vacuum tubes containing EDTA by the technique and values of erythrocyte osmotic fragility and complete blood count were determined. The main effect of age and gender with age x gender interaction has no significant effect on hematological parameters (P>0.05). The main effect of age and age x gender interaction of erythrocyte osmotic fragility was statistically significant and higher in the senior females at 0.7% and 0.9% (in addition gender) NaCl concentrations compared to the other groups (P<0.05). However, there was no statistical difference between the adult female and adult male and senior male group at 0.7% and 0.9% NaCl concentrations (P>0.05). The findings may be useful for veterinary clinical practice in establishing Kangal shepherd breed-specific reference ranges for some important hematological parameters.

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Effects of sex and age on the osmotic stability of Sahel goat erythrocytes

Cited by 12 publications

References 38 publications

First report of bovine anaemia associated Theileria sinensis infection and phylogenetic analyses of partial gene sequences of Theileria and Anaplasma species detected in naturally infected Malaysian cattle

First report of bovine anaemia associated Theileria sinensis infection and phylogenetic analyses of partial gene sequences of Theileria and Anaplasma species detected in naturally infected Malaysian cattle

Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle

Kangal Çoban Köpeklerinde Yaş ve Cinsiyetin Bazı Hematolojik Parametreler ve Eritrosit Ozmotik Kırılganlık Üzerine Etkisi

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