Effects of short-term exposure to mobile phone radiofrequency (900 MHz) on the oxidative response and genotoxicity in honey bee larvae

Vilić, Marinko; Gajger, Ivana Tlak; Tucak, Perica; Štambuk, Anamaria; Šrut, Maja; Klobučar, Göran; Malarić, Krešimir; Žaja, Ivona Žura; Pavelić, Ana; Manger, Marin; Tkalec, Mirta

doi:10.1080/00218839.2017.1329798

Cited by 19 publications

(13 citation statements)

References 46 publications

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“…However, it has been known that some parameters of oxidative stress, (CAT and GST activities, and MDA concentrations) may be infl uenced by numerous factors, such as living in greenhouses [54] or industrial areas [27], migratory beekeeping [55], seasonality [35,36], fl ying activities [36,56], aging [36,56] and brood rearing [36]. Although radio frequency electromagnetic fi eld of certain strength can infl uence oxidative stress, it is not likely to happen in realistic conditions [57]. A study of oxidative stress in honey bee drones suggests that survival of oxidative stress is due to tolerance of oxidative damage to lipids, rather than to its prevention or repair [58].…”

Section: Discussionmentioning

confidence: 99%

Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

et al. 2020

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Apart from the efficiency of coumaphos against Varroa mites, its impact on the oxidative status and survival of the honey bee (Apis mellifera) was assessed. The research was conducted on hives from the same apiary, equalised regarding the number of bees, brood area and food storage. Based on Varroa infestation the hives were allotted to two groups: non-infested (N) and infested (I). Both groups were either treated (T) – NT and IT, or untreated (U) – NU and IU. The treatment of infested bees was controlled with a follow-up treatment with amitraz. The efficiency of coumaphos was 96-97%. This organophosphate had a negligible effect on bee survival, but it significantly affected their oxidative status: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, and the concentrations of malonyl dialdehyde (MDA). Coumaphos significantly (p˂0.0001) decreased SOD activity in non-infested bees, but increased it in those infested. By contrast, both CAT and GST activities, as well as MDA concentrations significantly increased (from p˂0.05 to p˂0.0001) after treatment in all groups, with the exception of IT, where it declined. Coumaphos in non-infested hives caused oxidative stress per se, not unlike varroa in infested colonies. However, in infested colonies it decreased oxidative stress, owing to its efficacy against Varroa mites and contributed to the recovery of bee colonies. In spite of its certain downsides, coumaphos remains an effective anti-varroa substance, but should be used with precaution, not to add to the effects of environmental factors which may cause red-ox misbalance.

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Section: Discussionmentioning

confidence: 99%

Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

et al. 2020

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“…In the multi-stress site, we wanted to evaluate the cumulative effects of two different stress factors: the exposure to pesticides (the same as the chemical site) and the presence of an electromagnetic field generated by an electric transport line located above the experimental hives. Electromagnetic fields are known to cause different biological effects such as oxidative stress, genotoxic effects and immune system dysfunctions, all observed on different animal species [109]. The negative effects of electromagnetic radiation emitted by antennas, cell phones and high voltage power lines have been studied in humans [110][111][112][113] and in animals, including mice [114], bats [115], birds [116,117] and insects [118].…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Pesticides and Electromagnetic-Fields on Honeybees: Multi-Stress Exposure

Lupi

Mesiano

Adani

et al. 2021

Insects

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Honeybee and general pollinator decline is extensively reported in many countries, adding new concern to the general biodiversity loss. Many studies were addressed to assess the causes of pollinator decline, concluding that in most cases multi-stress effects were the most probable ones. In this research, the combined effects of two possible stress sources for bees, pesticides and electromagnetic fields (multi-stress conditions), were analyzed in the field. Three experimental sites were chosen: a control one far from direct anthropogenic stress sources, a pesticide-stress site and multi-stress one, adding to the same exposure to pesticides the presence of an electromagnetic field, coming from a high-voltage electric line. Experimental apiaries were monitored weekly for one year (from April 2017 to April 2018) by means of colony survival, queen activity, storage and brood amount, parasites and pathogens, and several biomarkers in young workers and pupae. Both exposure and effect biomarkers were analysed: among the first, acetylcholinesterase (AChE), catalase (CAT), glutathione S-transferase (GST) and alkaline phosphatase (ALP) and Reactive Oxygen Species (ROS); and among the last, DNA fragmentation (DNAFRAGM) and lipid peroxidation (LPO). Results showed that bee health conditions were the worst in the multi-stress site with only one colony alive out of the four ones present at the beginning. In this site, a complex picture of adverse effects was observed, such as disease appearance (American foulbrood), higher mortality in the underbaskets (common to pesticide-stress site), behavioral alterations (queen changes, excess of honey storage) and biochemical anomalies (higher ALP activity at the end of the season). The overall results clearly indicate that the multi-stress conditions were able to induce biochemical, physiological and behavioral alterations which severely threatened bee colony survival.

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“…Up until now, several terrestrial species of insects including economically relevant species were examined using the comet assay [25]. These include Dipterathe American serpentine leafminer (Liriomyza trifolii) [318]; Coleoptera -chestnut weevil (Curculio sikkimensis) [319], maize weevil (Sitophilus zeamais) [320,321], yellow fever mosquito (Aedes aegypti) [322] and cigarette beetle (Lasioderma serricorne) [323]; Lepidoptera -Indian meal moth (Plodia interpunctella) [324], diamondback moth (Plutella xylostella) [325], gypsy moth (Lymantria dispar) [326], common Mormon (Papilio polytes) [327], Oriental leafworm moth (Spodoptera litura) [328], beet armyworm (Spodoptera exigua) [329], Mediterranean flour moth (Ephestia kuehniella) [330], cotton bollworm (Helicoverpa armigera) [331,332] and corn stalk borer (Sesamia nonagrioides) [333]; Orthopteracommon field grasshopper (Chorthippus brunneus) [334][335][336], bow-winged grasshopper (Chorthippus biguttulus) [337], grasshopper (Aiolopus thalassinus) [338], desert locust (Schistocerca gregaria) [339], cave crickets (Dolichopoda laetitiae and D. geniculate) [340], house cricket (Acheta domesticus) [341]; Hemipterared cotton stainer (Dysdercus cingulatus) [327], and Hymenoptera -black garden ant (Lasius niger) [342] and honeybee (Apis mellifera) [343,344]. Besides terrestrial species, several aquatic species were assayed as well.…”

Section: Insectsmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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Effects of short-term exposure to mobile phone radiofrequency (900 MHz) on the oxidative response and genotoxicity in honey bee larvae

Cited by 19 publications

References 46 publications

Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

Combined Effects of Pesticides and Electromagnetic-Fields on Honeybees: Multi-Stress Exposure

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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