Effects of short-term heat stress on the activity of three antioxidant enzymes of predatory mite Neoseiulus barkeri (acari, phytoseiidae)

Li, Wei-Zhen; Zhu, Tong; Zhou, Jing‐Jiang; Shang, Suqin

doi:10.3389/fphys.2022.937033

Cited by 6 publications

(2 citation statements)

References 58 publications

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“…Additionally, at the same temperature, with the extension of duration time, SOD, POD, and CAT activity and the T-AOC were changed, which suggested that various exposure times also effect SOD, POD, and CAT activity and the T-AOC. Li et al found similar results in the study on the effects of short-term heat stress on the antioxidant enzymes of N. barkeri [ 51 ].…”

Section: Discussionsupporting

confidence: 60%

Elucidating the Effect of Temperature Stress on the Protein Content, Total Antioxidant Capacity, and Antioxidant Enzyme Activities in Tetranychus urticae (Acari: Tetranychidae)

Nie

Yang

Zhou

et al. 2023

Insects

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Tetranychus urticae Koch is a worldwide agricultural pest mite that feeds on more than 1100 kinds of crops. The mite has developed a high level of tolerance to high temperatures, but the physiological mechanism underlying the outstanding adaptability of this pest to high temperatures remains unclear. To clarify the physiological mechanisms of T. urticae in response to short-term heat stress, four temperatures (36, 39, 42, and 45 °C) and three short-term heat durations (2, 4, and 6 h) were conducted to test the effects on protein content, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and the total antioxidant capacity (T-AOC). The results showed that protein content, antioxidant enzyme activity, and T-AOC in T. urticae were significantly induced by heat stress. These results suggest that heat stress induces oxidative stress and that antioxidant enzymes play an important role in reducing oxidative damage in T. urticae. The data of this study will provide a basis for further research on the molecular mechanisms of thermostability and ecological adaptability of T. urticae.

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Section: Discussionsupporting

confidence: 60%

Elucidating the Effect of Temperature Stress on the Protein Content, Total Antioxidant Capacity, and Antioxidant Enzyme Activities in Tetranychus urticae (Acari: Tetranychidae)

Nie

Yang

Zhou

et al. 2023

Insects

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“…They typically use glutathione (GSH) as an electron donor [24]. Based on our previous research, short-term heat stress resulted in increased expression of SOD, CAT, and Prx activities in N. barkeri, and the enzyme activity reached its maximum at 40 • C-4 h [25]. A notable rise in antioxidant enzyme activities suggests the presence of oxidative stress and a positive capability to counteract it by eliminating ROS from cells [26,27].…”

Section: Introductionmentioning

confidence: 99%

Selection, Identification, and Transcript Expression Analysis of Antioxidant Enzyme Genes in Neoseiulus barkeri after Short-Term Heat Stress

Zhu,

Li,

Xue

et al. 2023

Antioxidants

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Phytoseiid mite Neoseiulus barkeri is a crucial biological control agent utilized to control pest mites and many insects in crops all over the world. However, they are vulnerable to multiple environmental pressures, with high-temperature stress being the most significant challenge. Heat stress disrupts the balance of reactive oxygen species (ROS) levels in organisms, resulting in oxidative stress within the body. Antioxidant enzymes play a crucial role in effectively neutralizing and clearing ROS. In this study, comparative transcriptomics and quantitative real-time PCR (qRT-PCR) were employed to assess the impact of short-term heat stress on the transcript expression of antioxidant enzyme genes in N. barkeri. We primarily identified four antioxidant enzyme genes (NbSOD, NbPrx, NbCAT, and NbGPX) in N. barkeri after exposure to short-term heat stress. Then, new data on the expression patterns of these genes were generated. RNA sequencing and bioinformatics analysis revealed that NbSOD belongs to the Fe/Mn family of superoxide dismutase (SOD), which was identified as MnSOD. NbPrx was classified as a 1-Cys peroxiredoxin of the peroxidase family, whereas NbCAT was recognized as a classical catalase, and NbGPX was determined as cytoplasmic glutathione peroxidase-1 (GPX1). Transcriptional expression analysis of these four genes was conducted at different high temperatures: 36 °C, 38 °C, and 40 °C for 2, 4, and 6 h. The results also showed that all four genes exhibited significant up-regulation in response to short-term heat stress. Similarly, the highest expression levels for NbSOD, NbPrx, and NbCAT were observed at 40 °C for 4 h. However, NbGPX displayed its maximum expression value at 38 °C for 4 h. Overall, the obtained data suggest that short-term heat stress increases levels of ROS generated inside living organisms, which disrupts the oxidative balance and leads to alterations in the expression levels of antioxidant enzyme genes.

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Transcriptome analysis revealed molecular basis of cold response in Prunus mume

et al. 2023

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Effects of short-term heat stress on the activity of three antioxidant enzymes of predatory mite Neoseiulus barkeri (acari, phytoseiidae)

Cited by 6 publications

References 58 publications

Elucidating the Effect of Temperature Stress on the Protein Content, Total Antioxidant Capacity, and Antioxidant Enzyme Activities in Tetranychus urticae (Acari: Tetranychidae)

Elucidating the Effect of Temperature Stress on the Protein Content, Total Antioxidant Capacity, and Antioxidant Enzyme Activities in Tetranychus urticae (Acari: Tetranychidae)

Selection, Identification, and Transcript Expression Analysis of Antioxidant Enzyme Genes in Neoseiulus barkeri after Short-Term Heat Stress

Transcriptome analysis revealed molecular basis of cold response in Prunus mume

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