The reduction in net CO2 emissions from increased C sequestration in soil and slower decomposition of SOM under most long-term no-till (NT) situations can potentially be offset by a concomitant increase in nitrous oxide (N2O) emissions after tillage reversal on long-term NT soils. The objective of this work was to quantify N2O emissions after tillage reversal on two contrasting western Canadian Prairie soils managed under long-term (~ 30 years) NT. We measured one growing season (2010) of soil N2O emissions on a Black Chernozem and Gray Luvisol at Ellerslie and Breton, AB, respectively following 30-years of NT and N fertilizer application at two rates (0 and 100 kg N ha-1) subjected to tillage reversal and no disturbance (i.e., continuing NT). Tillage reversal after long-term NT was associated with higher N2O emissions in both soils, but was significant only in the Gray Luvisol with 0 kg N ha-1. Long-term N fertilizer applications of 100 kg N ha-1 were associated with higher growing season soil N2O emissions and higher levels of soil N (i.e., a positive, long-term soil N balance) at both sites. Regardless of tillage, the difference in growing season nitrous oxide emissions from the 0 and 100 kg N ha-1 plots on the Gray Luvisol were much greater than the Black Chernozem. A modest increase in N2O emissions upon tillage reversal on a long-term NT soils could translate to a significant increase to agricultural greenhouse gas inventories in the event of large-scale tillage reversal on agricultural land in western Canada.