Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line

Liu, Li‐Li; Lin, Li‐Rong; Tong, Man‐Li; Fu, Zuo-Gen; Liu, Guili; Zheng, Weihong; Liu, Fan; Zhang, Dai-Wei; Zhang, Zhongying; Yang, Tian‐Ci

doi:10.3892/or.2012.1702

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…To our knowledge, there were few studies using lentivirus-mediated RNAi targeting COX-2 for determining the therapeutic significance of COX-2 in HCC. Liu et al, firstly explored the effects of silencing COX-2 expression via RNAi on the tumorigenicity of SMMC-7721 cell line 26. However, their study only provided primary results of the animal experiment rather than investigation of the detailed mechanism at the cellular level.…”

Section: Discussionmentioning

confidence: 99%

<p>Knockdown of cyclooxygenase-2 leads to growth inhibition and cell cycle arrest in hepatocellular carcinoma cells</p>

Chen

et al. 2019

OTT

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Background & aims: Cyclooxygenase-2 (COX-2) is proved to play important roles in the development and progression of various human tumors, including hepatocellular carcinoma (HCC). However, the antitumor effect of RNA interference (RNAi) technology targeting COX-2 in HCC has not yet been verified. Methods: We silenced COX-2 expression using a lentivirus-mediated RNAi and further investigated the effects of COX-2 knockdown on cell growth and cell cycle in Huh7 and SMMC-7721 cells. COX-2 mRNA was detected by RT-PCR while COX-2 protein was detected by Western blotting. The cell proliferation was measured by MTT assay. The cell cycle was measured by flow cytometry. The tumorigenicity of HCC cells was evaluated using soft-agar clonogenic assay in vitro and nude mouse xenograft model in vivo. Results: The down-regulation of COX-2 expression significantly inhibited cell proliferation and colony formation, and led to cell cycle arrest in vitro, and reduced the potential of tumorigenicity in vivo in both Huh7 and SMMC-7721 cells. Furthermore, PGE2 production was also decreased after COX-2 expression was suppressed. Finally, knockdown of COX-2 also induced the down-regulation of cell cycle-related protein, cyclinD1. Conclusions: The abrogation of COX-2 expression can lead to potent antitumor activity and knockdown of COX-2 may be served as a prospective therapeutic strategy against HCC.

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Section: Discussionmentioning

confidence: 99%

<p>Knockdown of cyclooxygenase-2 leads to growth inhibition and cell cycle arrest in hepatocellular carcinoma cells</p>

Chen

et al. 2019

OTT

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“…Hepatocellular carcinoma very often presents at an advanced stage and has a very poor prognosis [45]. GPS and five other kinds of traditional Chinese medicine extracts can lesion SMMC-7721 human hepatocellular carcinoma cells; however, the exact mechanism of action is not yet known [46]. 4.12.5.…”

Section: Hepatocellular Carcinomamentioning

confidence: 99%

“…The major anti-cancer properties of GPS that have been proven in vitro are listed in Figure 6. extracts can lesion SMMC-7721 human hepatocellular carcinoma cells; however, the exact mechanism of action is not yet known [46]. 4.12.5.…”

Section: Hepatocellular Carcinomamentioning

confidence: 99%

Gentiopicroside—An Insight into Its Pharmacological Significance and Future Perspectives

Antoniadi,

Bartnik,

Angelis

et al. 2023

Cells

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Gentiopicroside (GPS) is a leading component of several plant species from the Gentianaceae botanical family. As a compound with plenty of biological activities and a component of herbal drugs, GPS has an important role in the regulation of physiological processes in humans. The results of recently published scientific studies underline a meaningful role of this molecule as an active factor in metabolic pathways and mechanisms, which may have an influence in the treatment of different diseases, including digestive tract disorders, malignant changes, neurological disorders, microbial infections, bone formation disorders, inflammatory conditions, and others. This review aims to collect previously published reports on the biological properties of GPS as a single compound that were confirmed by in vitro and in vivo studies, and to draw attention to the newly discovered role of this bitter-tasting secoiridoid. Thanks to these properties, the research on this substance could be revisited.

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“…Hepatocellular carcinoma presents very often at an advanced stage and has a very poor prognosis [48]. GPS and five other kinds of traditional Chinese medicine extracts can lesion SMMC-7721 human hepatocellular carcinoma cells; however, the exact mechanism of action is not yet known [49]. 4.14.5 Other types of cancer GPS was proved to exhibit anticancer effect when tested in other cancer models as well.…”

Section: Hepatocellular Carcinomamentioning

confidence: 99%

Gentiopicroside—The Biologically Active Plant Metabolite—An Insight into Its Pharmacological Significance and Future Perspectives

Antoniadi,

Bartnik,

Angelis

et al. 2023

Preprint

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Gentiopicroside (GPS) is a leading component of several plant species from Gentianaceae botanical family. As a compound with plenty biological activities and a component that is often consumed in dietary supplements and herbal drugs, GPS has an important role in the regulation of physiological processes in humans. Recent studies on the type 2 receptors shed new light on GPS applications showing its agonistic properties that could be meaningful in the future therapeutic strategies of Parkinson’s disease, thyroid disorders or cancer states. This review aims to collect previously published reports on the biological properties of GPS as a single compound that were confirmed by in vitro and in vivo studies, and to draw attention at the newly discovered role of this bitter-tasting secoiridoid as a type 2 receptor agonist. Thanks to these properties, the research on this substance could be revisited.

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Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line

Cited by 3 publications

References 18 publications

<p>Knockdown of cyclooxygenase-2 leads to growth inhibition and cell cycle arrest in hepatocellular carcinoma cells</p>

<p>Knockdown of cyclooxygenase-2 leads to growth inhibition and cell cycle arrest in hepatocellular carcinoma cells</p>

Gentiopicroside—An Insight into Its Pharmacological Significance and Future Perspectives

Gentiopicroside—The Biologically Active Plant Metabolite—An Insight into Its Pharmacological Significance and Future Perspectives

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