Effects of silencing endothelin-1 on invasion and vascular formation in lung cancer

Zhang, Zhenyu; Chen, Lili; Xu, Wei; Sigdel, Keshavraj; Jiang, Xing‐Tang

doi:10.3892/ol.2017.6027

Cited by 12 publications

(10 citation statements)

References 53 publications

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“…Recently, several studies have proved the aberrant expression of ET-1 in multiple cancers such as ovarian cancer ( 8 ), prostate cancer ( 9 ), breast cancer ( 10 ) and colon cancer ( 11 ). Moreover, ET-1 plays an important role in the development and progression of various tumors through mediating cell proliferation, migration and invasion, for example, knockdown of ET-1 significantly inhibited lung cancer cell proliferation and invasion ( 12 ). Said et al showed that silencing of ET-1 decreased tumor metastasis of bladder cancer ( 13 ) and osteosarcoma ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

ET-1 promotes the growth and metastasis of esophageal squamous cell carcinoma via activating PI3K/Akt pathway

Yin¹,

Wang²,

Li³

et al. 2020

Transl Cancer Res TCR

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Background Esophageal squamous cell carcinoma (ESCC) is one of the prevalent and deadly cancers worldwide. Previous studies confirmed that endothelin-1 (ET-1) serves as an oncogene and therapeutic target in various tumors. However, the role and mechanism of ET-1 in the progression of ESCC remains largely unclear. Methods Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA level of ET-1 in ESCC tissues and cell lines. Cell counting kit-8 (CCK-8), flow cytometry and Transwell assay were performed to examine the proliferation, cell cycle arrest, invasion and migration capacity of ESCC cells. Western blot was applied to measure the expression of ET-1 and PI3K/Akt pathway-related proteins. Furthermore, we also assessed the effect of ET-1 on tumor growth in vivo . Results ET-1 was highly expressed in ESCC tissues and associated with poor outcomes. Knockdown of ET-1 significantly inhibited the proliferation, migration and invasion capacity of ESCC cells and promoted cell cycle arrest. Mechanistically, silencing of ET-1 exerts anti-proliferation and anti-metastasis activities via inactivation of the PI3K/Akt signaling pathway in ESCC in vitro and in vivo . Conclusions These findings uncover the effective suppression of cell proliferation and metastasis through silencing of ET-1 and blocking the PI3K/Akt signaling pathway, which is an attractive therapeutic regimen for the treatment of ESCC.

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Section: Introductionmentioning

confidence: 99%

ET-1 promotes the growth and metastasis of esophageal squamous cell carcinoma via activating PI3K/Akt pathway

Yin¹,

Wang²,

Li³

et al. 2020

Transl Cancer Res TCR

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“…Its inhibition (by riluzole) significantly handicaps the process of new vessel formation [77]. Endothelin-3, a vasoactive peptide released by vascular smooth muscle cells and endothelin converting enzyme-1, a protease that activates endothelin-3 also play a role in angiogenesis [78,79,80] and especially in tumor angiogenesis [81,82,83,84,85]. See Scheme 1.…”

Section: Introductionmentioning

confidence: 99%

Synergy Between Low Dose Metronomic Chemotherapy and the pH-Centered Approach Against Cancer

Koltai¹,

Cardone

Reshkin

2019

IJMS

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Low dose metronomic chemotherapy (MC) is becoming a mainstream treatment for cancer in veterinary medicine. Its mechanism of action is anti-angiogenesis by lowering vascular endothelial growth factor (VEGF) and increasing trombospondin-1 (TSP1). It has also been adopted as a compassionate treatment in very advanced human cancer. However, one of the main limitations of this therapy is its short-term effectiveness: 6 to 12 months, after which resistance develops. pH-centered cancer treatment (pHT) has been proposed as a complementary therapy in cancer, but it has not been adopted or tested as a mainstream protocol, in spite of existing evidence of its advantages and benefits. Many of the factors directly or indirectly involved in MC and anti-angiogenic treatment resistance are appropriately antagonized by pHT. This led to the testing of an association between these two treatments. Preliminary evidence indicates that the association of MC and pHT has the ability to reduce anti-angiogenic treatment limitations and develop synergistic anti-cancer effects. This review will describe each of these treatments and will analyze the fundamentals of their synergy.

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“…It is formed by endothelial cells and consists of 21 amino acids, and 4 isomers ET-1, ET-2, ET-3, and ET-4. In addition to its vasoconstrictive effect, it plays a crucial role in promoting DNA synthesis, expression of proto-oncogene and cell proliferation owing to its growth factor-like effect (4). ET can play a crucial role in tumor angiogenesis through binding to the surface receptors, endothelin receptor A (ETAR) and endothelin receptor B (ETBR), in a paracrine or autocrine manner (5) Tumor angiogenesis and metastatic spread are two interconnected processes, which may contribute to cancer associated death.…”

Section: Introductionmentioning

confidence: 99%

Ezh2, endothelin-1, and cd34 as biomarkers of aggressive cervical squamous cell carcinoma: an immunohistochemical study

Fathy

Abdelrahman²

2018

TJPATH

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Objective: Cervical cancer has an increasing incidence in developing countries with a predominance of squamous cell carcinoma. In this work, we aimed to analyze the role of EZH2, Endothelin-1, and CD34 as indicators of the aggressiveness in cervical squamous cell carcinoma. Material and Method:Immunohistochemical expression of EZH2, Endothelin-1, and CD34 was studied in 54 paraffin-embedded tissue specimens of cervical squamous cell carcinoma. Their correlation to the clinicopathologic features and the potential angiogenic role were analyzed.Results: High EZH2 expression was noted in 78% of cervical squamous cell carcinoma with a significant relation with tumor grade, FIGO stage and lymph node metastasis (p=<0.001, p=0.007, p=0.03 respectively). Endothelin-1 overexpression was detected in 63% of the studied cases with a significant association with tumor size, FIGO stage and lymph node metastasis (p=0.009, p=0.002, p=0.02 respectively). High CD34 expression (MVD) was noted in 56% of the cases and associated with the tumor size, FIGO stage and lymph node metastasis (p<0.001, p<0.001, p=0.04 respectively). The three markers were significantly associated (p<0.05).Conclusion: EZH2, ET-1, and CD34 may act as biomarkers of aggressive cervical squamous cell carcinoma. They may contribute to the signaling pathway of angiogenesis. Therefore, they could potentially be used in targeted therapy.

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Effects of silencing endothelin-1 on invasion and vascular formation in lung cancer

Cited by 12 publications

References 53 publications

ET-1 promotes the growth and metastasis of esophageal squamous cell carcinoma via activating PI3K/Akt pathway

ET-1 promotes the growth and metastasis of esophageal squamous cell carcinoma via activating PI3K/Akt pathway

Synergy Between Low Dose Metronomic Chemotherapy and the pH-Centered Approach Against Cancer

Ezh2, endothelin-1, and cd34 as biomarkers of aggressive cervical squamous cell carcinoma: an immunohistochemical study

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