Effects of silver nanocolloids on plant complex type N-glycans in Oryza sativa roots

Horiuchi, Risa; Nakajima, Yukari; Kashiwada, Shosaku; Miyanishi, Nobumitsu

doi:10.1038/s41598-018-19474-z

Cited by 7 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, a recent study reported a high abundance of PMGs (76.7% of the N ‐glycome) in ungerminated Oryza sativa seeds, which decreased dramatically to 36.1% after 48 h of germination (Horiuchi, Hirotsu, & Miyanishi, ). The PMGs were proposedly carried by vacuolar‐resident storage proteins utilised for growth and development during germination (Horiuchi et al ., ), and were further suggested to be synthesised and preserved in the rice germ until germination (Ying et al ., ). These findings suggest that GnT‐I, Hex and the other glyco‐enzymes facilitating PMP synthesis in plants (see Section II.1 a ) are active during seed development.…”

Section: Surveying Pmps Across the Eukaryotic Kingdoms And Phylamentioning

confidence: 99%

Human protein paucimannosylation: cues from the eukaryotic kingdoms

et al. 2019

View full text Add to dashboard Cite

Paucimannosidic proteins (PMPs) are bioactive glycoproteins carrying truncated α-or β-mannosyl-terminating asparagine (N )-linked glycans widely reported across the eukaryotic domain. Our understanding of human PMPs remains limited, despite findings documenting their existence and association with human disease glycobiology. This review comprehensively surveys the structures, biosynthetic routes and functions of PMPs across the eukaryotic kingdoms with the aim of synthesising an improved understanding on the role of protein paucimannosylation in human health and diseases. Convincing biochemical, glycoanalytical and biological data detail a vast structural heterogeneity and fascinating tissue-and subcellular-specific expression of PMPs within invertebrates and plants, often comprising multi-α1,3/6-fucosylation and β1,2-xylosylation amongst other glycan modifications and non-glycan substitutions e.g. O-methylation. Vertebrates and protists express less-heterogeneous PMPs typically only comprising variable core fucosylation of bi-and trimannosylchitobiose core glycans. In particular, the Manα1,6Manβ1,4GlcNAc(α1,6Fuc)β1,4GlcNAcβAsn glycan (M2F) decorates various human neutrophil proteins reportedly displaying bioactivity and structural integrity demonstrating that they are not degradation products. Less-truncated paucimannosidic glycans (e.g. M3F) are characteristic glycosylation features of proteins expressed by human cancer and stem cells. Concertedly, these observations suggest the involvement of human PMPs in processes related to innate immunity, tumorigenesis and cellular differentiation. The absence of human PMPs in diverse bodily fluids studied under many (patho)physiological conditions suggests extravascular residence and points to localised functions of PMPs in peripheral tissues. Absence of PMPs in Fungi indicates that paucimannosylation is common, but not universally conserved, in eukaryotes. Relative to human PMPs, the expression of PMPs in plants, invertebrates and protists is more tissue-wide and constitutive yet, similar to their human counterparts, PMP expression remains regulated by the physiology of the producing organism and PMPs evidently serve essential functions in development, cell-cell communication and host-pathogen/symbiont interactions. In most PMP-producing organisms, including humans, the N -acetyl-β-hexosaminidase isoenzymes and linkage-specific α-mannosidases are glycoside hydrolases critical for generating PMPs via N -acetylglucosaminyltransferase I (GnT-I)-dependent and GnT-I-independent truncation pathways. However, the identity and structure of many species-specific PMPs in eukaryotes, their biosynthetic routes, strong tissue-and development-specific expression, and diverse functions are still elusive. Deep exploration of these PMP features involving, for example, the characterisation of endogenous PMP-recognising lectins across a variety of healthy and N -acetyl-β-hexosaminidase-deficient human tissue types and identification of microbial adhesins reactive to human PMPs, are amongs...

show abstract

Section: Surveying Pmps Across the Eukaryotic Kingdoms And Phylamentioning

confidence: 99%

Human protein paucimannosylation: cues from the eukaryotic kingdoms

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Glycoconjugates are widely present in animal [78], as well as plant [79][80][81][82][83] and microbial [84][85][86][87] structural and functional systems [88]. Many of the plant glycan antigens are on the cell wall [89,90].…”

Section: Glycans Are Ubiquitousmentioning

confidence: 99%

Recent Advances In Nutritional Sciences: An Overview Of Glycans And MiRNAs

Menapace¹

2018

J Nutr Food Sci

View full text Add to dashboard Cite

There are many nutritional substances that humans consume on a daily basis: water, carbohydrates lipids and proteins are main biochemical components of food. But others in a smaller amount are vitamins minerals and enzymes. At a possibly lesser quantity are glycans and miRNAs. The presence of oligoglycans in all food sources has been an established fact for many years. These special carbohydrates are present as glycoconjugates (glycoproteins or glycolipids) in and on the surface of all the cells (glycocalyx) of all organisms that we eat and remain intact through the GI tract as we lack the enzymatic repertoire of the human body to unbind their particular β-linkages. Glycans bind to naturally present human lectins (through protein-carbohydrate interactions), but also with other human glycans (through carbohydrate-carbohydrate interactions, or CCI). Moreover, these glycans, like fibres, are digested by the gut microbiota that resides within the intestine. As our biochemistry shapes the composition of the microbiome, so does the composition of glycans and foods that we consume, triggering biological responses. miRNAs are small, single-stranded, 19-to 23-nucleotide-long RNA molecules and affect the stability of messenger RNA (mRNA) influencing protein synthesis. miRNAs are also present in foods and act on both the microbial composition in our gut and may be absorbed by the walls of the GI tract, demonstrating resistance to food processing and enzymatic attack. Though still a topic of controversy, these small, noncoding RNAs that control gene expression may directly enter into the circulating miRNA population of dietary exogenous miRNAs. It can hence be possible to identify a relationship between glycans and miRNAs in food on one side, microbiota composition on the other and the resultant health status of the host (immune system), on the third side.

show abstract

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Harvey

2021

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.

show abstract

Effects of silver nanocolloids on plant complex type N-glycans in Oryza sativa roots

Cited by 7 publications

References 32 publications

Human protein paucimannosylation: cues from the eukaryotic kingdoms

Human protein paucimannosylation: cues from the eukaryotic kingdoms

Recent Advances In Nutritional Sciences: An Overview Of Glycans And MiRNAs

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Contact Info

Product

Resources

About