Effects of silver nanoparticles on human dermal fibroblasts and epidermal keratinocytes

Galandáková, Adéla; Franková, Jana; Ambrožová, Nikola; Habartová, Klára; Pivodová, Veronika; Zálešák, Bohumil; Šafářová, Klára; Smékalová, Monika; Ulrichová, Jitka

doi:10.1177/0960327115611969

Cited by 59 publications

(52 citation statements)

References 42 publications

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“…A number of in vitro studies have been performed exploring the effects of AgNPs on a variety of cell types [88,239,244,245,246,247,248,249,250,251,252,253]. The most common mechanisms of toxicity from nanosized silver particles, as well as silver ions released from them [245,254] are: oxidative stress [246,255], Trojan-horse mechanisms [256,257] and DNA damage [163].…”

Section: Potential Side Effects Of Silver Nanoparticlesmentioning

confidence: 99%

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

et al. 2016

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Prosthetic joint infection (PJI) is a feared complication of total joint arthroplasty associated with increased morbidity and mortality. There is a growing body of evidence that bacterial colonization and biofilm formation are critical pathogenic events in PJI. Thus, the choice of biomaterials for implanted prostheses and their surface modifications may significantly influence the development of PJI. Currently, silver nanoparticle (AgNP) technology is receiving much interest in the field of orthopaedics for its antimicrobial properties and a strong anti-biofilm potential. The great advantage of AgNP surface modification is a minimal release of active substances into the surrounding tissue and a long period of effectiveness. As a result, a controlled release of AgNPs could ensure antibacterial protection throughout the life of the implant. Moreover, the antibacterial effect of AgNPs may be strengthened in combination with conventional antibiotics and other antimicrobial agents. Here, our main attention is devoted to general guidelines for the design of antibacterial biomaterials protected by AgNPs, its benefits, side effects and future perspectives in PJI prevention.

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Section: Potential Side Effects Of Silver Nanoparticlesmentioning

confidence: 99%

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

et al. 2016

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“…Any detrimental effects of these antimicrobial dressings on healing may exacerbate the nonhealing phenotype observed in chronic wounds. Thus, the effects of silver dressings on healing subsequent to infection clearance, and recommendations for their prophylactic use to prevent infection, remain unclear …”

mentioning

confidence: 99%

Silver oxysalts promote cutaneous wound healing independent of infection

Thomason

Lovett²,

Spina

et al. 2018

Wound Repair Regeneration

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Chronic wounds often exist in a heightened state of inflammation whereby excessive inflammatory cells release high levels of proteases and reactive oxygen species (ROS). While low levels of ROS play a fundamental role in the regulation of normal wound healing, their levels need to be tightly regulated to prevent a hostile wound environment resulting from excessive levels of ROS. Infection amplifies the inflammatory response, augmenting levels of ROS which creates additional tissue damage that supports microbial growth. Antimicrobial dressings are used to combat infection; however, the effects of these dressing on the wound environment and healing independent of infection are rarely assessed. Cytotoxic or adverse effects on healing may exacerbate the hostile wound environment and prolong healing. Here we assessed the effect on healing independent of infection of silver oxysalts which produce higher oxidative states of silver (Ag /Ag ). Silver oxysalts had no adverse effect on fibroblast scratch wound closure whilst significantly promoting closure of keratinocyte scratch wounds (34% increase compared with control). Furthermore, dressings containing silver oxysalts accelerated healing of full-thickness incisional wounds in wild-type mice, reducing wound area, promoting reepithelialization, and dampening inflammation. We explored the mechanisms by which silver oxysalts promote healing and found that unlike other silver dressings tested, silver oxysalt dressings catalyze the breakdown of hydrogen peroxide to water and oxygen. In addition, we found that silver oxysalts directly released oxygen when exposed to water. Collectively, these data provide the first indication that silver oxysalts promote healing independent of infection and may regulate oxidative stress within a wound through catalysis of hydrogen peroxide.

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“…All wounds healed at similar rates and with similar cosmetic results in terms of scarring (Figure 6a-c). While the Tegaderm dressing was found to have more complete epidermal surface coverage on wound biopsies on PWD 7 (Figure 7a), the slower rate of reepithelialization with the PLGA/PEG/Ag dressings is not surprising given the known inhibitory effects of silver on keratinocyte, fibroblasts, and epidermal cells 52,53 and did not have a negative effect on outcomes. Future studies could probe the antimicrobial efficacy of PLGA/PEG/Ag in vivo with an infection challenge model or by quantifying the bacterial burden in wounds over time.…”

Section: Discussionmentioning

confidence: 90%

Sprayable and biodegradable, intrinsically adhesive wound dressing with antimicrobial properties

Daristotle

Lau

Erdi

et al. 2019

Bioengineering & Transla Med

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Conventional wound dressings are difficult to apply to large total body surface area (TBSA) wounds, as they typically are prefabricated, require a layer of adhesive coating for fixation, and need frequent replacement for entrapped exudate. Large TBSA wounds as well as orthopedic trauma and low-resource surgery also have a high risk of infection. In this report, a sprayable and intrinsically adhesive wound dressing loaded with antimicrobial silver is investigated that provides personalized fabrication with minimal patient contact. The dressing is composed of adhesive and biodegradable poly(lactic-co-glycolic acid) and poly(ethylene glycol) (PLGA/PEG) blend fibers with or without silver salt (AgNO 3 ). in vitro studies demonstrate that the PLGA/PEG/Ag dressing has antimicrobial properties and low cytotoxicity, with antimicrobial silver controllably released over 7-14 days. In a porcine partial-thickness wound model, the wounds treated with both antimicrobial and nonantimicrobial PLGA/PEG dressings heal at rates similar to those of the clinical, thin film polyurethane wound dressing, with similar scarring. However, PLGA/PEG adds a number of features beneficial for wound healing: greater exudate absorption, integration into the wound, a 25% reduction in dressing changes, and tissue regeneration with greater vascularization. There is also modest improvement in epidermis thickness compared to the control wound dressing. K E Y W O R D Santimicrobial wound dressings, biodegradable polymers, silver, solution blow spinning, wound healing

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Effects of silver nanoparticles on human dermal fibroblasts and epidermal keratinocytes

Cited by 59 publications

References 42 publications

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

Silver oxysalts promote cutaneous wound healing independent of infection

Sprayable and biodegradable, intrinsically adhesive wound dressing with antimicrobial properties

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