Effects of Silybum marianum Aqueous Extract and L-carnitine on Stereological Changes in Diazinon-Treated Rat Liver

Abstract

As an organophosphorus, Diazinon (DZN) impairs liver tissue function by inhibiting acetylcholinesterase and causing oxidative stress. In this study, the effects of <em>Silybum</em><em> marianum </em>aqueous extract (SMAE) and L-carnitine (LC) on the stereological and histopathological changes of the liver in DZN-treated male rats were investigated. The rats in this study were placed into 9 groups of 8 each containing control, placebo, and a combination of DZN, SMAE, and LC. The animals received SMAE and chemicals orally for 30 days. At last, the liver tissue of all animals was removed. Then, tissue sections from the liver were provided to study the stereological markers including liver volume and weight, hepatocytes’ volume, central venous volume, sinusoidal volume, connective tissue volume, inflammation rate, and a number of the hepatocytes’ nuclei. Also, the sample tissues were evaluated histopathologically. Treatment with DZN significantly reduced the liver volume and weight, hepatocyte volume, central venous volume, sinusoidal volume, and hepatocyte nucleus number compared to placebo and control but it significantly increased the inflammation and volume of liver’s connective tissue. However, co-administration of SMAE and LC with DZN improved liver volume and weight, hepatocyte volume, central venous volume, sinusoidal volume, connective tissue volume, and hepatocyte nucleus number alone compared to the DZN treatment. Liver inflammation was also significantly decreased compared to the DZN treatment but comparing to the placebo and control groups, it increased significantly. Simultaneous administration of SMAE and LC has protective effects on liver tissue and can reduce DZN-induced liver injury in rats.

Restorative effects of Silymarin milk thistle on the liver following paracetamol-induced hepatotoxicity in adult albino rats

“…From literature reviewed, it was found out that post-administration of silymarin milk thistle on carbon tetrachloride induced liver fibrosis, liver morphological parameters were greatly improved, leading to resolution of liver fibrosis (Tsai et al, 2008), hence concurring with this present study that demonstrates restorative effects of silymarin milk thistle on paracetamol-induced hepatotoxicity. Upon administration of silymarin milk thistle to diazinon-treated rats, it was found that liver volume and weight increased significantly as compared to those that never received silymarin, concurring with this study that liver volume and weight were positively impacted by the administration of silymarin milk thistle (Masoumi et al, 2020).…”

Efek Pemberian Ekstrak Etanol Biji Corchorus Capsularis Terhadap Kadar Ast Dan Alt Tikus Yang Diinduksi Diazinon

“…Hasil yang diperoleh kontradiksi dengan penelitian lain yang melaporkan bahwa esensial oil dari beberapa tanaman memiliki aktivitas antioksidan dan dapat digunakan dalam pencegahan toksisitas diazinon (Al-Attar, Elnaggar and Almalki, 2017). Selain itu dilaporkan juga bahwa L-carnitin dan Silybum marianum yang memiliki aktivitas antioksidan dapat memberikan perlindungan pada jaringan hati dan mengurangi cedera hati akibat induksi diazinon pada tikus (Masoumi, Shariati and Mokhtari, 2020). Peningkatan kadar AST dan ALT pada tikus dalam penelitian ini mengindikasikan terjadi kerusakan jaringan hati akibat induksi diazinon.…”