Effects of Sinensetin on Lipid Metabolism in Mature 3T3‐L1 Adipocytes

Kang, Seong-Il; Shin, Hye-Sun; Ko, Hee-Chul; Kim, Se-Jae

doi:10.1002/ptr.4683

Cited by 33 publications

(25 citation statements)

References 10 publications

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“…Instead, studies on human subcutaneous fat cells have been shown that synephrine has a lypolitic action . A recent study, conducted on 3T3‐L1 cells, demonstrated an antiadipogenetic effect of CA extracts, downregulating C/EBPβ through inhibition of Akt phosphorylation , therefore, preventing the expression of forkhead transcription factor Foxo1 (FKHR) . The main role of Foxo1 is to block adipocytes differentiation, thus, the final consequence of Akt inactivation CA‐mediated is to inhibit PPARγ and C/EBPα expression .…”

Section: Nutraceuticals Targeting Adipocyte Life Cyclementioning

confidence: 99%

Nutraceuticals and regulation of adipocyte life: Premises or promises

Colitti

Grasso

2014

BioFactors

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Obesity is the actual worldwide health threat, that is associated with an increased number of metabolic disorders and diseases. Following the traditional hypothesis stating that in obesity hypertrophic adipocytes trigger the adipose tissue hyperplasia, strategies to treat obesity have increased fat researches of the molecular processes that achieve adipocyte enlargement and formation that finally increase body fat mass. Moreover, a new cell type was recently identified, the "brite" adipocyte that presents a unique gene expression profile of compared to both brown and white adipocytes. Therapies against obesity, targeting these cells and their pathways, would include the induction of lipolysis and apoptosis or the inhibition of differentiation and adipogenesis. However, it should be noted that both the increase of adipocyte size and number take place in association with positive energy balance. According to the adipose tissue expansion hypothesis, adipogenesis could be related with improved metabolic health of obese people, taking back the adipose mass to a traditionally site of lipid storage. Furthermore, new perspectives in fat biology suggest that the conversion of white-to-brown adipocytes and their metabolism could be exploited for the development of therapeutic approaches against obesity-associated diseases and for the regulation of energy balance. Drugs currently available to treat obesity generally have unpleasant side effects. A novel promising approach is the usage of dietary supplements and plant products that could interfere on the life cycle of adipocyte. Here, various dietary bioactive compounds that target different stages of adipocyte life cycle and molecular and metabolic pathways are reviewed.

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Section: Nutraceuticals Targeting Adipocyte Life Cyclementioning

confidence: 99%

Nutraceuticals and regulation of adipocyte life: Premises or promises

Colitti

Grasso

2014

BioFactors

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“…18) Sinensetin significantly and dose-dependently increased cAMP levels at 30 min after treatment (Fig. 5B).…”

Section: Signals At An Early Stagementioning

confidence: 83%

“…We have recently reported that sinensetin had an anti-inflammatory effect in lipopolysaccharide-stimulated RAW 264.7 cells, 17) and it enhanced fatty acid β-oxidation in mature 3T3-L1 adipocytes. 18) In the present study, we investigated the effects of sinensetin on adipogenesis in 3T3-L1 preadipocytes and lipolysis in mature 3T3-L1 adipocytes, and characterized the underlying molecular mechanisms.…”

mentioning

confidence: 99%

Sinensetin Enhances Adipogenesis and Lipolysis by Increasing Cyclic Adenosine Monophosphate Levels in 3T3-L1 Adipocytes

Kang

Shin

Kim

2015

Biological & Pharmaceutical Bulletin

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“…AMPK activity modulates numerous aspects of the mammalian cell metabolism, and it also has diverse roles that extend from energy metabolism through to transcriptional control. In addition, AMPK functions modulate fatty acid oxidation, glycolysis, fatty acid and cholesterol synthesis (20,21). In order to investigate whether WEP regulates adipogenesis in differentiated 3T3-L1 adipocytes, the effects of WEP on the phosphorylation of AMPK were investigated.…”

Section: Discussionmentioning

confidence: 99%

Water‑extracted plum (Prunus salicina L. cv. Soldam) attenuates adipogenesis in murine 3T3‑L1 adipocyte cells through the PI3K/Akt signaling pathway

2017

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Abstract. The objective of the present study was to evaluate the effects of water-extracted plum (WEP) on adipocyte differentiation, adipogenesis and inflammation in differentiated 3T3-L1 adipocyte cells. WEP was assessed for basic analyses, including high-performance liquid chromatography, total phenolic and flavonoid content and antioxidant activity [1,1-diphenyl-2-picrylhydrazyl (DPPH) assays] in vitro. Moreover, the cell viability was measured using an MTT assay. Adipogenesis and lipid accumulation in 3T3-L1 adipocytes was investigated using Oil Red O staining, and the expression of genes and proteins associated with adipogenesis and lipolysis were examined by reverse transcription polymerase chain reaction and western blotting. In addition, sulforaphane using a positive control was performed simultaneously. The WEP significantly suppressed adipocyte differentiation and lipid accumulation in differentiated adipocytes without cytotoxicity. WEP resulted in direct anti-obesity effects through the modulation of adenosine monophosphate-activated protein kinase, sterol regulatory element-binding protein 1c, cytidine-cytidine-adenosine-adenosine-thymidine/enhancer binding protein α and peroxisome proliferator-activated receptor γ. These regulations of molecular expressions were significantly activated via the phosphoinositide 3-kinase/Akt pathway. Moreover, these results provide potential anti-adipogenic effects of WEP and may have potential as a natural agent for the prevention and improvement of obesity. IntroductionFood intake behaviors, genes and the environment are important factors in causing people to be overweight and obese. The prevalence of obesity worldwide has rapidly increased over the past decades. In 2014, the National Health and Nutrition Survey in Korea reported that over a third of Korean adults (30.2%) were obese (1,2). Obesity-related conditions include heart disease, stroke, type 2 diabetes and certain types of cancer, which give rise to complex health issues, including hypertension, sleep apnoea, arthritis and decrease future life expectancy (3,4).Prior studies have demonstrated that numerous dietary polyphenolic constituents derived from plants are more effective antioxidants in vitro compared with vitamin E or C (5). In addition, increasing interest has been paid to dietary agents such as fruits and vegetables for the possibility of controlling obesity as lipolysis agents, since these dietary agents are known to have or enhance anti-adipogenic and/or lipolysis activity without causing cytotoxicity (6). Plum (Prunus salicina L. cv. Soldam) is an important fruit crop grown in Gimcheon, Gyungbuk, Korea. It is often used to help regulate the function of the digestive system and has been recently used as a supplement for irregularity treatment (7). This effect has been attributed to various compounds present in the fruits, such as dietary fiber, isatin and sorbitol (8). In addition, prunes and prune juice are often used to help regulate the functioning of the digestive system. Dried plums (or pru...

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Effects of Sinensetin on Lipid Metabolism in Mature 3T3‐L1 Adipocytes

Cited by 33 publications

References 10 publications

Nutraceuticals and regulation of adipocyte life: Premises or promises

Nutraceuticals and regulation of adipocyte life: Premises or promises

Sinensetin Enhances Adipogenesis and Lipolysis by Increasing Cyclic Adenosine Monophosphate Levels in 3T3-L1 Adipocytes

Water‑extracted plum (Prunus salicina L. cv. Soldam) attenuates adipogenesis in murine 3T3‑L1 adipocyte cells through the PI3K/Akt signaling pathway

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