Effects of single and multi-strain probiotics on the growth, hemato-immunological, enzymatic activity, gut morphology and disease resistance in Rohu, Labeo rohita

Saravanan, K.; Sivaramakrishnan, T.; Praveenraj, J.; Kiruba-Sankar, R.; Haridas, Harsha; Kumar, Shailesh; Varghese, Benny

doi:10.1016/j.aquaculture.2021.736749

Cited by 24 publications

(8 citation statements)

References 50 publications

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“…However, supplementation of B. tequilensis as water additive was also effective when used at a higher potency (WA3 group, 10 11 cfu/ ml culture water) and produced positive effects comparable to the feed supplement group D2. Although weight gain recorded for rohu in the present report seemed to be lower in comparison to the other species (e.g., Nile tilapia, Zhou et al, 2010), it was comparable with erstwhile studies conducted with rohu under captive condition (Bhujel et al, 2020;Mohapatra et al, 2014;Mukherjee et 2019;Saravanan et al, 2021). Majority of the preceding studies considered a potency that ranged between 10 6 and 10 10 cfu/g for dietary application of Bacillus probiotics (singly or in combination) and recorded significant growth improvements in Nile tilapia, O. niloticus (Aly et al, 2008;Liu et al, 2017); common carp, Cyprinus carpio (Ye et al, 2011); rohu, Labeo rohita (Giri et al, 2014;Kumar et al, 2006;Mukherjee et al, 2019); and grass carp, Ctenopharyngodon idella (Tang et al, 2019;Wu et al, 2012;Xue et al, 2020).…”

Section: Discussionsupporting

confidence: 80%

Improvement of growth, nutrient utilization and haemato‐immunological parameters in rohu, Labeo rohita (Hamilton) using Bacillus tequilensis (KF623287) through diets or as water additive

Dutta

Ghosh

2021

Aquaculture Nutrition

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The study appraised effects of Bacillus tequilensis (KF623287), through diets or water, on the growth and health status of rohu, Labeo rohita fingerlings. A basal diet (35% protein) was fed to the control (without B. tequilensis supplementation) and other experimental groups in triplicate for 60 days. B. tequilensis was added either to the diets at 1 × 10 7 cfu/g (D1), 1 × 10 9 cfu/g (D2) and 1 × 10 11 cfu/g (D3) or to the rearing water at 1 × 10 7 cfu/ml (WA1), 1 × 10 9 cfu/ml (WA2) and 1 × 10 11 cfu/ml (WA3) every 2 days. Results indicated that group D2 had significantly (p < .05) higher weight gain (115 ± 3.25%) and improved feed utilization than other experimental groups.However, group WA3 (109 ± 3.16% weight gain) appeared as the second best after D2 group. Further, improved haematological parameters, serum lysozyme, peroxidase and superoxide anion production along with increased phagocytic and respiratory burst activities were noticed in fish that received B. tequilensis either through diets (D2) or as water additive (WA3). Increased serum IgM was noticed at day 30, while decreased thereafter. The study revealed that B. tequilensis, either through diets or as water, could improve growth, nutrient utilization and immunity in L. rohita fingerlings.However dietary administration of probiotics at a lower dose was more effective than the water additive.

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Section: Discussionsupporting

confidence: 80%

Improvement of growth, nutrient utilization and haemato‐immunological parameters in rohu, Labeo rohita (Hamilton) using Bacillus tequilensis (KF623287) through diets or as water additive

Dutta

Ghosh

2021

Aquaculture Nutrition

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“…Previous studies also reported a significant effect of Bacillus spp. on growth, disease resistance, and hematological parameters of Nile tilapia (Soltan et al 2016;Sutthi et al 2018;Elsabagh et al 2018;Kuebutornye et al 2020;Won et al 2020;Ghalwash et al 2021) and other aquatic species (Hauville et al 2016;Munir et al 2016;da Paixão et al 2017;Kong et al 2017;Wang et al 2017;Amoah et al 2021;Saravanan et al 2021). In this present study, the effect of two previously isolated Bacillus spp.…”

Section: Introductionmentioning

confidence: 47%

Effect of probiotic Bacillus spp.-supplemented feed on the growth, length-weight relationship, and condition factor of Nile tilapia (Oreochromis niloticus)

Samson

2022

J Exp Bio & Ag Sci

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This study was carried out to evaluate the effect of two probiotic Bacillus spp. (RM10 and BFAR9) on the growth, length-weight relationship (LWR), and condition factor (k) of Nile tilapia (Oreochromis niloticus). For this, one hundred thirty-five fingerlings (1.12±0.08 g weight and 1.26±0.15 cm length) were divided into three groups (Control, RM10, and BFAR9) and distributed into nine circular concrete tanks. The fish were fed with commercial (control) and Bacillus spp. supplemented diets at 5% of body weight for 56 days. The results of the study revealed better (P<0.05) growth concerning average body weight (ABW - 17.12±0.71g), specific growth rate (SGR - 4.89±0.22 g·day-1), absolute growth (AG - 16.02±0.78 g), and feed conversion ratio (FCR - 1.31±0.09) in the group fed with Bacillus sp. RM10 as compared to the control (ABW- 13.25±2.34g; SGR - 4.41±0.17g·day-1; AG - 12.13±2.25g; FCR - 1.62±0.11). The LWR in all experimental treatments showed a significant correlation (P<0.05) with an R2 value of 0.988, 0.966, and 0.979 for Control, RM10, and BFAR9, respectively. The k value revealed that all treatments are in good condition as k value is greater than 1 (1.913, 2.038, and 1.896 for control, RM10, and BFAR9 respectively). The result of the current study revealed that application of Bacillus sp. RM10 improves the growth and feed utilization in Nile tilapia.

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“…Multi-strain probiotics (MSP) have been used in aquaculture to improve aquatic animal growth, illness resistance, and immune response while also enhancing water quality variables [ 21 , 22 , 23 , 24 , 25 ]. To construct a functional MSP, a few prerequisites must be met.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance

Puvanasundram

Chong

Sabri

et al. 2022

Biology

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Compatibility of each strain in a multi-strain probiotic (MSP), along with its properties, becomes a strong base for its formulation. In this study, single-strain probiotics (SSPs) and multi-strain probiotics (MSPs) were evaluated in vitro for strain compatibility, microbial antagonism, biofilm formation capacity, and stress tolerance. Bacillus amyloliquefaciens L11, Enterococcus hirae LAB3, and Lysinibacillus fusiformis SPS11 were chosen as MSP1 candidates because they showed much stronger antagonism to Aeromonas hydrophila and Streptococcus agalactiae than a single probiotic. MSP 2 candidates were Lysinibacillus fusiformis strains SPS11, A1, and Lysinibacillus sphaericus strain NAS32 because the inhibition zone produced by MSP 2 against Vibrio harveyi and Vibrio parahaemolyticus was much higher than that produced by its constituent SSPs. MSP1 in the co-culture assay reduced (p < 0.05) A. hydrophila count from 9.89 ± 0.1 CFU mL−1 to 2.14 ± 0.2 CFU mL−1. The biofilm formation of both MSPs were significantly higher (p < 0.05) than its constituent SSPs and the pathogens. The SSPs in both MSPs generally showed resistance to high temperatures (80, 90, and 100 °C) and a wide range of pH (2 to 9). This in vitro assessment study demonstrates that MSP1 and 2 have the potential to be further explored as multi-strain probiotics on selected aquatic species.

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Effects of single and multi-strain probiotics on the growth, hemato-immunological, enzymatic activity, gut morphology and disease resistance in Rohu, Labeo rohita

Cited by 24 publications

References 50 publications

Improvement of growth, nutrient utilization and haemato‐immunological parameters in rohu, Labeo rohita (Hamilton) using Bacillus tequilensis (KF623287) through diets or as water additive

Improvement of growth, nutrient utilization and haemato‐immunological parameters in rohu, Labeo rohita (Hamilton) using Bacillus tequilensis (KF623287) through diets or as water additive

Effect of probiotic Bacillus spp.-supplemented feed on the growth, length-weight relationship, and condition factor of Nile tilapia (Oreochromis niloticus)

Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance

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