Effects of skeletal element identity, delipidation and demineralization on the analysis of stable isotope ratios of C and N in fish bone

Bas, María; Cardona, Luís

doi:10.1111/jfb.13521

Cited by 12 publications

(18 citation statements)

References 56 publications

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“…The C baseline may change because of changes in total primary productivity, in the relative contribution of several types of primary producers (Hill, McQuaid, & Kaehler, ) and also because of the Suess effect, caused by the massive release of 13 C‐depleted CO 2 to the atmosphere because of the massive burning of fossil fuels since the Industrial Revolution (Eide, Olsen, Ninnemann, & Eldevik, ). Research dealing with zooarchaeological samples usually correct for the Suess effect (e.g., Braje et al, ; Chamberlain et al, ; Szpak et al, ; Zangrando, Riccialdelli, et al, ), but often forget about the impact of variables amounts of lipids on the δ 13 C values of skeletal remains (Bas & Cardona, ; Guiry et al, ). As a result, lipid removal from zooarchaeological samples is still uncommon and likely to confound the interpretation of results (e.g., Braje et al, ; Robson et al, ; Szpak et al, ; Szpak, Orchard, Salomon, & Gröcke, ).…”

Section: Discussionmentioning

confidence: 99%

“…The raw data were recalculated taking into account a linear regression previously calculated for isotopic reference materials (Skrzypek, ). Following Bas and Cardona (), only δ 13 C dml and δ 15 N bulk values were used for latter analysis. Furthermore, the carbon to nitrogen (C:N) atomic ratio of each dml subsample (see Supporting Information Table S1) was used to assess the efficiency of lipid extraction (DeNiro, ).…”

Section: Methodsmentioning

confidence: 99%

“…It should be noted that the published stable isotope ratios of modern grenadier and eelpout were obtained from demineralized bone (Zangrando, Riccialdelli, et al, ) and muscle samples (Riccialdelli et al, ), respectively. Therefore, to allow comparison with the stable isotope ratios of bulk bones, values were transformed according to the published offset between tissues (Ankjærø, Christensen, & Grønkjær, ) and treatments (Bas & Cardona, ).…”

Section: Methodsmentioning

confidence: 99%

“…Once dry, each sample was ground to fine powder and divided into two subsamples. This is because calcium carbonate and lipids have to be removed to obtain unbiased δ 13 C values (Bas & Cardona, 2018;Guiry, Szpak, & Richards, 2016;Newsome, Koch, Etnier, & Aurioles-Gamboa, 2006), but demineralization consistently increases bone collagen δ 15 N values (Bas & Cardona, 2018). One subsample (bulk hereafter) was ground to fine powder with mortar and pestle and approximately 0.7 mg of ancient and modern bone powder was weighed into 3.3 × 5 mm tin cups, 7 mg of modern shell powder was weighed into 5 × 8 mm tin cups and 14 mg of ancient shell powder was weighed into 5 × 8 mm tin cups.…”

Section: Stable Isotope Analysismentioning

confidence: 99%

“…The chloroform:methanol solution was changed overnight until it was transparent. Bone dml subsamples were dried again for 24 hr at 60°C and demineralized with 0.5 N hydrochloric acid (HCl) until no more CO 2 bubbles were released (Bas & Cardona, 2018;Newsome et al, 2006). Shell dml subsamples were first demineralized by soaking in 1 N HCl until no more CO 2 was released (Saporiti, Bearhop, et al, 2014).…”

Section: Stable Isotope Analysismentioning

confidence: 99%

See 4 more Smart Citations

Back to the future? Late Holocene marine food web structure in a warm climatic phase as a predictor of trophodynamics in a warmer South‐Western Atlantic Ocean

Bas

Godino

Álvarez

et al. 2018

Global Change Biology

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Stable carbon and nitrogen isotope ratios in the skeletal elements of both ancient and modern marine species from the Beagle Channel were used to compare the structure of Late Holocene and modern food webs, and predict potential changes as a result of a Sea Surface Temperature (SST) increase in the region. Complementary, ancient and modern shells of limpets and mussels were isotopically analysed to explore changes in the isotopic baseline and compare marine food webs through time after an appropriate correction for baseline shifts. Results confirmed a declining pattern of marine primary productivity during the Late Holocene in the Beagle Channel. In general, the isotopic niches overlapped largely in the ancient food web in comparison to the current marine one, with the exception of that of cormorants (Phalacrocorax sp.). Our data suggest that all the species that have undergone intense human exploitation (Arctocephalus australis, Otaria flavescens and Merluccius sp.) significantly increased their trophic levels. The most important finding of this work was the very high isotopic overlap between snoek (Thyrsites atun) and hake (Merluccius sp.) during the Late Holocene. Increasing SST as a result of global warming could favour the recolonization of the southern South‐Western Atlantic Ocean by snoek from the South‐Eastern Pacific Ocean, with a potential impact on the landings of the economically important Argentine and Austral hake. These findings highlight the relevance of using zooarchaeological remains for providing predictions about marine food webs changes in the near future.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Stable Isotope Analysismentioning

confidence: 99%

Section: Stable Isotope Analysismentioning

confidence: 99%

See 3 more Smart Citations

Back to the future? Late Holocene marine food web structure in a warm climatic phase as a predictor of trophodynamics in a warmer South‐Western Atlantic Ocean

Bas

Godino

Álvarez

et al. 2018

Global Change Biology

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Isotopic ecology of Hawaiian green sea turtles (Chelonia mydas) and reliability of δ13C, δ15N, and δ34S analyses of unprocessed bone samples for dietary studies

et al. 2023

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This study conducted stable isotope analysis (δ13C, δ15N, and δ34S) on the epidermis and two skeletal elements (rib and squamosal bones) of Hawaiian green turtles (Chelonia mydas) and putative diet items obtained from two neritic sites: the Kona/Kohala coast and Oahu. Turtle tissues were collected in 2018–2020 and diet samples in 2018, 2019, and 2021. The effect of body size and sampling locality on individual bulk tissue isotope values was evaluated, and stable isotope mixing models based on δ13C, δ15N, and δ34S values from those tissues and four groups of food sources were used to reconstruct diet histories of the turtles. Mixing models indicated that green turtles along the Kona/Kohala coast consumed an omnivorous diet, whereas those from Oahu had an herbivorous diet. These diet make-ups are consistent with published gut content analyses. However, mixing models using the stable isotope ratios in rib and squamosal bone failed to yield reasonable diet histories, probably due to inadequacies of the applied trophic discrimination factor (TDF), a key model parameter. These results further establish that stable isotope ratios in the epidermis can be used effectively to study green turtle diet, but also reveal that more validation—and establishment of appropriate TDFs—is needed before bone can be used reliably to assess green turtle diet.

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Predicting habitat use by the Argentine hake Merluccius hubbsi in a warmer world: inferences from the Middle Holocene

et al. 2020

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Fish skeletal remains recovered from two archaeological sites dated in the Middle Holocene of Tierra del Fuego (Argentina) were analysed to describe habitat use patterns by hake in the past and predict changes in a warmer world. Mitochondrial DNA was successfully extracted and amplified from 42 out of 45 first vertebra from ancient hake and phylogenetic analysis assigned all haplotypes to Argentine hake (Merluccius hubbsi). According to osteometry, the Argentine hake recovered from the archaeological site were likely adults ranging 37.2-58.1 cm in standard length. C and N stable isotope analysis showed that currently Argentine hake use foraging grounds deeper than those of Patagonian blenny and pink cusk-eel. Argentine hake, however, had a much broader isotopic niche during the Middle Holocene, when a large part of the population foraged much shallower than contemporary pink cusk-eel. The overall evidence suggests the presence of large numbers of Argentine hake onshore Tierra del Fuego during the Middle Holocene, which allowed exploitation by hunter-gatherer-fisher groups devoid of fishing technology. Interestingly, average SST off Tierra del Fuego during the Middle Holocene was higher than currently (11 °C vs 7 °C) and matched SST in the current southernmost onshore spawning aggregations, at latitude 47 °S. This indicates that increasing SST resulting from global warming will likely result into an increased abundance of adult Argentine hake onshore Tierra del Fuego, as during the Middle Holocene. Furthermore, stable isotope ratios from mollusc shells confirmed a much higher marine primary productivity during the Middle Holocene off Tierra del Fuego. Keywords Hake • Osteometry • Stable isotopes • Tierra del Fuego • ZooarchaeologyCommunicated by Leon A. Barmuta.

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Effects of skeletal element identity, delipidation and demineralization on the analysis of stable isotope ratios of C and N in fish bone

Cited by 12 publications

References 56 publications

Back to the future? Late Holocene marine food web structure in a warm climatic phase as a predictor of trophodynamics in a warmer South‐Western Atlantic Ocean

Back to the future? Late Holocene marine food web structure in a warm climatic phase as a predictor of trophodynamics in a warmer South‐Western Atlantic Ocean

Isotopic ecology of Hawaiian green sea turtles (Chelonia mydas) and reliability of δ13C, δ15N, and δ34S analyses of unprocessed bone samples for dietary studies

Predicting habitat use by the Argentine hake Merluccius hubbsi in a warmer world: inferences from the Middle Holocene

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