Effects of sodium alginate elicitation on secondary metabolites and antioxidant activity of safflower genotypes under in vitro salinity stress

Golkar, Pooran; Taghizadeh, Marzieh; Noormohammadi, A.

doi:10.1007/s11627-019-10008-4

Cited by 18 publications

(9 citation statements)

References 48 publications

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“…The PAL is considered as an important enzyme in the phenylpropanoid pathway and involved with the plant cells defense response to ROS. The PAL activity was also considered as an e cient marker for identifying the intensity of environmental stresses in plant species (Golkar et al 2019b). The phenolics are secondary metabolites with protective effects against oxidative stress (Manquián-Cerda et al 2016;Maqsood et al 2014).…”

Section: Effect Of the Smfs And Mnps On The Antioxidant Defense Mechanismmentioning

confidence: 99%

Modification of phytochemical production and antioxidant activity of Dracocephalum kotschyi cells by exposure to static magnetic field and magnetite nanoparticles

Taghizadeh

Nasibi

Kalantari

et al. 2021

Plant Cell Tiss Organ Cult

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Dracocephlum kotschyi Boiss is a genus in Lamiaceae family and a medicinal herb native to Iran. The cell suspension cultures were treated by static magnetic eld (SMF) and Fe 3 O 4 magnetite nanoparticles (MNP) to understand the production yield of secondary metabolites. The treatment procedure was done by cultivating the cells either with 100 ppm MNP, SMFs, or simultaneous exposure to both MNP and SMFs. The SMF at 30 mT was uniformly applied to the cells either for 3 or 4 days with a 3 h per day or a 5 h per day intervals, respectively. The contents of phenolics and phytochemicals were then examined by high performance liquid chromatography and UV-Vis spectrophotometer. These treatments imposed oxidative stress and induced polyphenol oxidase and phenylalanine ammonia lyase, accompanied by enhanced production of phenolics, anthocyanins, avonoids, and lignin. The highest membrane embrittlement and elicitation was found upon simultaneous application of the MNPs and SMFs, followed by the MNP and SMFs. The contents of naringin, rosmarinic acid, quercetin, thymol, carvacrol, apigenin, and rutin increased in the intracellular biomass of all treated cells and extracellular culture media. These ndings propose the potential of these elicitors in simultaneous production and secretion of these phytochemicals into culture media.

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Section: Effect Of the Smfs And Mnps On The Antioxidant Defense Mechanismmentioning

confidence: 99%

Modification of phytochemical production and antioxidant activity of Dracocephalum kotschyi cells by exposure to static magnetic field and magnetite nanoparticles

Taghizadeh

Nasibi

Kalantari

et al. 2021

Plant Cell Tiss Organ Cult

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“…The weight was recorded at T0, corresponding to initial transfer on fresh medium, as well as after 7 and 14 days for chilling stress, and 14, and 28 days for salt stress. Growth rate was calculated as mean callus relative growth rate (GR) by [(T final − T initial)/T initial] × 100% according to what reported by [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Abiotic Stresses Elicitation Potentiates the Productiveness of Cardoon Calli as Bio-Factories for Specialized Metabolites Production

et al. 2022

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Cultivated cardoon (Cynara cardunculus L. var altilis) is a Mediterranean traditional food crop. It is adapted to xerothermic conditions and also grows in marginal lands, producing a large biomass rich in phenolic bioactive metabolites and has therefore received attention for pharmaceutical, cosmetic and innovative materials applications. Cardoon cell cultures can be used for the biotechnological production of valuable molecules in accordance with the principles of cellular agriculture. In the current study, we developed an elicitation strategy on leaf-derived cardoon calli for boosting the production of bioactive extracts for cosmetics. We tested elicitation conditions that trigger hyper-accumulation of bioactive phenolic metabolites without compromising calli growth through the application of chilling and salt stresses. We monitored changes in growth, polyphenol accumulation, and antioxidant capability, along with transcriptional variations of key chlorogenic acid and flavonoids biosynthetic genes. At moderate stress intensity and duration (14 days at 50–100 mM NaCl) salt exerted the best eliciting effect by stimulating total phenols and antioxidant power without impairing growth. Hydroalcoholic extracts from elicited cardoon calli with optimal growth and bioactive metabolite accumulation were demonstrated to lack cytotoxicity by MTT assay and were able to stimulate pro-collagen and aquaporin production in dermal cells. In conclusion, we propose a “natural” elicitation system that can be easily and safely employed to boost bioactive metabolite accumulation in cardoon cell cultures and also in pilot-scale cell culture production.

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“…Sodium alginate has the potential as an elicitor to increase plant tolerance in stressful environments such as drought and salinity. (Salachna et al 2018in Golkar et al, 2019. According to a study by Golkar et al (2019), safflower callus induced by the addition of sodium alginate 0.075% and 0.15% inside the culture medium under salinity stress produced more secondary metabolites (total phenolic content (TPC), total flavonoids (TFD), total flavonols (TFL), anthocyanin (Ant)) and had antioxidant activity (total antioxidant capacity (TAC), phenylalanine ammonia-lyase (PAL), catalase (CAT)) higher than non-salinity and non-elicitation.…”

Section: Cytotoxicity Of Control and Treatment Callusmentioning

confidence: 99%

“…Furthermore, according to Augereau et al, (1986), there was a difference in the total alkaloid levels in Catharanthus roseus G. Son C4 strain after storage at 15°C for 7 weeks. On the other hand, a study in callus of safflower showed that sodium alginate increases the antioxidant compounds under salinity stress, suggesting sodium alginate as a potent elicitor (Golkar et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation

Tunjung

Fajarina²,

Prabowo³

et al. 2021

Indonesian J Pharm

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Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using callus preservation. Our preliminary study used two preservation methods of callus, which are stored in 4 0C with or without alginate encapsulation. However, low temperatures and alginate encapsulation can be stress factors for plants, affecting the production of bioactive compounds and their anticancer ability. This study's objective was to determine the bioactive compounds of callus with and without preservation and their effect on the cytotoxicity against cancer cells. GC-MS detected the bioactive compounds in the extract, and cytotoxicity of callus against breast cancer (T47D), and non-cancer cell (Vero) is tested using MTT method. The results showed that preservation in 4 0C with and without encapsulation caused a change in bioactive compounds' profile. The change mainly precursor compound in callus, after being preserved and recultured, then changes into intermediates or final compounds, indicating the difference in growth phases. The terpenoid compounds detected after preservation are squalene and geranyl linalool. However, Kaffir lime callus extract pre and post preservation are not cytotoxic to both cancer and non-cancer cells. Therefore, the preservation method did not act as an elicitor to callus.

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Effects of sodium alginate elicitation on secondary metabolites and antioxidant activity of safflower genotypes under in vitro salinity stress

Cited by 18 publications

References 48 publications

Modification of phytochemical production and antioxidant activity of Dracocephalum kotschyi cells by exposure to static magnetic field and magnetite nanoparticles

Modification of phytochemical production and antioxidant activity of Dracocephalum kotschyi cells by exposure to static magnetic field and magnetite nanoparticles

Abiotic Stresses Elicitation Potentiates the Productiveness of Cardoon Calli as Bio-Factories for Specialized Metabolites Production

Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation

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