Effects of sodium dodecyl sulfate on wheat (TriticumAestivumL.) seedlings

Abstract

In order to evaluate the phytotoxicity of the anionic surfactant sodium dodecyl sulfate (SDS), the inhibition of wheat seed germination was investigated. The effects of SDS on antioxidant enzyme activities [catalase and gluthione reductase (GR)], protein, soluble sugar, and proline in wheat (Triticum aestivum L.) seedlings were investigated after 6 days of SDS stress. With the increase in the SDS concentration, the root length of the wheat seedlings were inhibited markedly, and the protein content of the shoot decreased gradually. SDS stress caused significant changes in GR activity, soluble sugar, and proline contents in the shoots and roots of wheat. Accumulations of proline were observed in the shoots and roots and SDS caused growth inhibition on the wheat seedling. © 2015 American Institute of Chemical Engineers Environ Prog, 34: 1142–1147, 2015

Evaluation of physiological and biochemical aberration linked to effect of sodium dodecyl sulphate on barley seedlings

“…It was also determined that the levels of reduction in growth exhibited a positive correlation with the increase in SDS dosage. As compatible with our results, Chang et al [19] reported that SDS reduced the growth of wheat. It is possible that the SDS-induced growth retardation is due to destructions on the synthesis reactions.…”

“…Also, it was determined in vitro that SDS breaks down the peptide bonds in amino acids and therefore denatures the proteins [25]. Our results clearly pointed out that SDS led to the critical decline in soluble protein content, corroborating previous result recorded for wheat grown in the presence of SDS [19]. The possible cause of these declines could be the disruptions in the cellular metabolic events during transcription, translation and post-translation.…”

“…Unlike POX, the activity of CAT, responsible for the prevention of the hydroxyl radical formation by catalysing the conversion of H 2 O 2 into water and oxygen [6], was seriously reduced by SDS applications. This result has corroborated the result of a previous study which recorded a decline of CAT activity in wheat in the presence of SDS [19]. After the exposure of plants to SDS, the inactivation of CAT might result from the separation from the enzyme molecules of the hem groups which is essential for CAT activation [41,68].…”

“…The possible cause of these declines could be the disruptions in the cellular metabolic events during transcription, translation and post-translation. Previous studies also showed that SDS-induced decreases in plant protein contents are associated with the disruption of peptide bonds after translation [19,22]. Along with protein content, the protein profile images obtained from electrophoresis confirmed the negative effects of SDS on protein biosynthesis.…”

“…In these studies, it has been reported that SDS led to an important decrease in the growth rates and chlorophyll contents of duckweed and water fern [26,27]. Another study has also revealed that the SDS caused the serious declines in biomass, germination percentage, CAT activity and protein content in wheat [19]. However, there is no study evaluating the effects of SDS-induced changes in protein profile, free radical and membrane damage of plants.…”