Effects of soil amendments combined with solarization on the soil microbial community in strawberry cultivation using quantitative real-time PCR

Özyilmaz, Ümit; Benlioğlu, Kemal; Yıldız, Ayhan; Benlioğlu, Hatice Seher

doi:10.1007/s12600-016-0552-z

Cited by 16 publications

(9 citation statements)

References 65 publications

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“…Following the logic of Hypothesis 1, we had expected the opposite temporal trend: that biological activity would be reduced during treatment, but would return to control levels rapidly thereafter. Though we do not have a mechanistic explanation for this result, potential contributing factors include changes in microbial community composition [19,55] or the soil chemical environment [15][16][17][18] during and after solarization.…”

Section: Comparing Solarization To Tarpingmentioning

confidence: 89%

“…The 17.9% organic matter measured in our FIELD experiment, though still within the range found on operating organic farms in our region [52], is quite high. Incorporation of organic amendments prior to solarization, termed biosolarization, is known to decrease the time needed for thermal inactivation of weed seeds [53] and can lead to enhanced control of soilborne pathogens as well [12,54,55]. Though the practice is promising, more work evaluating the impact of biosolarization on beneficial soil microbiota is advised; Kanaan et al found that soil biological activity was reduced during and for four weeks following solarization integrated with compost application [56].…”

Section: Field and Greenhouse Solarizationmentioning

confidence: 99%

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Effects of Field and Greenhouse Solarization on Soil Microbiota and Weed Seeds in the Northeast USA

Birthisel

Smith

Mallory

et al. 2019

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Soil solarization using clear plastic is a promising weed management strategy for organic farms in the Northeast USA. Based on grower concerns that the practice might negatively affect beneficial soil microbiota, we conducted experiments to measure the effects of 2 and 4 weeks of solarization in a field and a closed greenhouse. Soil microbial communities were assayed by dilution plating on semi-selective agar media. Populations of general bacteria, general fungi, bacilli, and florescent pseudomonads were unaffected by field solarization, but fluorescent pseudomonads were reduced following greenhouse solarization. At plastic removal, soil biological activity was reduced non-significantly in the field and by 45% in the greenhouse. Soil biological activity fluctuated following field solarization, being significantly suppressed at 5 but not 14 days after plastic removal. In the greenhouse, biological activity remained suppressed up to 28 days after plastic removal. Solarization increased available nitrogen in the field and greenhouse. Four weeks of solarization reduced viability of buried weed seeds by 64% in the field and 98% in the greenhouse, indicating that the practice can cause substantial weed seed mortality. Maximum soil temperatures, measured at 10 cm depth under solarization, were 44 • C in the field and 50 • C in the greenhouse; temperatures were theoretically sufficient for the reduction of some soil borne pathogens. A subsequent experiment measured the effects of solarization and tarping (black plastic) on soil biological activity. During mulching, biological activity was unaffected by treatment, but 14 days after plastic removal, biological activity was reduced in the solarized treatment as compared with the control. Overall, these results suggest that solarization can deplete the weed seedbank. Although soil biological activity was reduced by solarization, it may bounce back after a period. Greenhouse solarization achieved higher temperatures and was more lethal to weed seeds and some microbiota than field solarization.

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Section: Comparing Solarization To Tarpingmentioning

confidence: 89%

Section: Field and Greenhouse Solarizationmentioning

confidence: 99%

Effects of Field and Greenhouse Solarization on Soil Microbiota and Weed Seeds in the Northeast USA

Birthisel

Smith

Mallory

et al. 2019

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“…The marker gene was also used in a similar study to estimate the number of strawberry pathogens in the soil samples, including Verticillium spp. The method was able to detect 17.7 pg of the V. dahliae DNA [81]. The ITS marker was also applied in the quantification of V. dahliae in affected strawberry roots and soil.…”

Section: Detection Methods Of Plant Pathogenic Fungal Speciesmentioning

confidence: 99%

“…The assay was performed in field conditions with remarkable reliability [89]. The quantification of soil-borne diseases on strawberry fruit was performed with the application of ITS1 primers as described previously by Lieven’s team [80,81]. In agreement with their discoveries, in the Ozyilmaz study, the marker was specific for at least 5 of the Verticillium species.…”

Section: Detection Methods Of Plant Pathogenic Fungal Speciesmentioning

confidence: 99%

“…In agreement with their discoveries, in the Ozyilmaz study, the marker was specific for at least 5 of the Verticillium species. The reaction detected 0.6 pg of pathogen DNA [81]. The identification of Verticillium species in soil was also performed by the Tzelepis’ team using a qPCR assay with newly designed primers for V. dahliae, V. longisporum, V. tricorpus and V. albo-atrum .…”

Section: Detection Methods Of Plant Pathogenic Fungal Speciesmentioning

confidence: 99%

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Alternative Molecular-Based Diagnostic Methods of Plant Pathogenic Fungi Affecting Berry Crops—A Review

2019

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Increasing consumer awareness of potentially harmful pesticides used in conventional agriculture has prompted organic farming to become notably more prevalent in recent decades. Central European countries are some of the most important producers of blueberries, raspberries and strawberries in the world and organic cultivation methods for these fruits have a significant market share. Fungal pathogens are considered to be the most significant threat to organic crops of berries, causing serious economic losses and reducing yields. In order to ameliorate the harmful effects of pathogenic fungi on cultivations, the application of rapid and effective identification methods is essential. At present, various molecular methods are applied for fungal species recognition, such as PCR, qPCR, LAMP and NGS.

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Sustainable Management of Soil-Borne Plant Pathogens

Ibrahim

Mostafa

Abdellatif

et al. 2022

Earth Systems Protection and Sustainability

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Effects of soil amendments combined with solarization on the soil microbial community in strawberry cultivation using quantitative real-time PCR

Cited by 16 publications

References 65 publications

Effects of Field and Greenhouse Solarization on Soil Microbiota and Weed Seeds in the Northeast USA

Effects of Field and Greenhouse Solarization on Soil Microbiota and Weed Seeds in the Northeast USA

Alternative Molecular-Based Diagnostic Methods of Plant Pathogenic Fungi Affecting Berry Crops—A Review

Sustainable Management of Soil-Borne Plant Pathogens

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