Solute, nutrient and bacterial inputs to the River Rhone from the interstitial habitat of a gravel bar and the floodplain aquifer were investigated during an artificial drought . Eight springs were investigated : four groundwater-fed springs in the floodplain, located at the bottom of the bank ; and four interstitial-fed springs located at the downstream end of a gravel bar. During this period, the inflows of groundwater to the river represented an average input of 0 .77 mg 1-1 of nitrogen (of which 93 .3% were nitrates), 0 .0187 mg 1-1 of total phosphorus (of which 42 .2% was orthophosphate), 3 .56 mg 1 -' of silica, 2 .315 ± 0 .703 mg 1 -1 of dissolved organic carbon (DOC, of which 47% was biodegradable) and 7 .3 x 104 ± 3 .7 x 10 4 bacteria per ml (of which 8 .8% were active) . Silica, DOC, biodegradable DOC, and bacteria concentrations displayed temporal variations during the study, which seem to be linked to the biological activity of the groundwater biofilm. There was a strong heterogeneity between the two types of groundwater that flow to the river : concentrations of calcium and alkalinity were higher in bank springs than in gravel bars springs . In these latters, sulfate, sodium, nitrogen, phosphorus were significantly higher .