In order to understand the application of laser spot high precision detection, a laser spot high precision detection based on Gaussian spot model was proposed. Laser spot center location is one of the key techniques in optical measurement. Firstly, by analyzing the common positioning algorithms, this paper presents a Gaussian fitting method using the unsaturation point in the spot image and taking the amplitude point of the fitting function as the center of the spot. Secondly, artificial light spot is used to verify the algorithm. The results show that the error of the algorithm is much less than 0.1 pixel, and it is a feasible spot center location algorithm.