Effects of somatostatin on gastrointestinal hormone-induced glycogenolysis and gluconeogenesis in cultured liver cells

Abstract

When isolated rat liver cells were incubated for 15 min in the presence of vasoactive intestinal peptide, gastric inhibitory polypeptide, secretin or glucagon at a concentration of 2.0 micrograms/ml, glycogenolysis was stimulated by 30%-67% above the control. Slight but significant increase on gluconeogenesis was also observed by the addition vasoactive intestinal peptide, gastric inhibitory polypeptide or secretin. Somatostatin inhibited both glycogenolysis and gluconeogenesis induced by these hormones, but the degrees of inhibition are clearly much higher in the hormone-induced gluconeogenesis than glycogenolysis, and no significant inhibition of glycogenolysis was observed in case of glucagon and VIP. These results suggest the possibility that the so-called enterohepatic axis may play a part of roles in the regulation of serum glucose levels through gastrointestinal hormones belonging to the secretin family, and that it may be further regulated by somatostatin through gluconeogenesis.

Physiology and Pharmacology of LHRH and Somatostatin

“…It even blocks ion transport in the colon (134). Somatostatin inhibits gluconeogenesis in cultured liver cells in response to VIP, GIP, and secretin (135). .…”

A review on the involvement of catecholamines in animal behaviour

“…Intermittent tail-shock stress in mice increases extracellular dopamine relative to baseline values and it is concluded that dopamine concentration increases during adverse conditions (Abercrombie et al, 2006). Dopamine is involved in an animal's attraction to a stimulus that predicts reward (Lacourt & Tarrant, 1985) and is involved in gluconeogenesis (Matsumura et al, 1980). O'Neill (2016) showed that cattle breed types differ in terms of dopamine secretion.…”

“…Aversive conditions (such as transport and handling at an abattoir) increase urinary dopamine, as described for other species by Abercrombie et al (2006). Dopamine has been shown to be involved in gluconeogenesis (Matsumura et al, 1980) to make glucose available to an animal to cope with its environment. Dopamine is an important neurotransmitter, which is involved in an animal's attraction to a stimulus that predicts reward (Parkinson et al, 2002).…”