Effects of some gelling agents and their concentrations on conversion of oil palm polyembryoids into plantlets

Palanyandy, Sharrmila Rengeswari; Gantait, Saikat; Sinniah, Uma Rani

doi:10.1186/s43141-019-0018-z

Cited by 9 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies were also extended to investigate a wide range of plant species and crops in an attempt to screen the best ingredients to be used in explants and medium components. Our study has also produced convincing results consistent with those obtained with bananas (Kacar et al 2010), wheat (Malik et al 2017), kiwi (Mardiana et al 2018), oil and date palms (Al-Mayahi and Ali 2021; Palanyandy et al 2020), and rice (Gehad M. Mohamed et al 2021;Repalli et al 2019).…”

Section: Mechanical Strengthsupporting

confidence: 88%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

View full text Add to dashboard Cite

Bacterial cellulose (BC) is a natural biodegradable, eco-friendly fiber, lying within the nanoscale range. It is reputable for its various physical and chemical qualities, like high hydrophilicity, immense crystallinity, ease of sterility, being toxin-free, and extremely pure. Adding to its wide applicability in different fields, this study evaluated the applicability of a developed gelling substitute for plant tissue culture media. The BC matrix was characterized under the acronym PLATIBACGEL (PLAnt TIssue Culture BActerial Cellulose GEL), formed by Komagataeibacter hansenii AS.5, preisolated from rotten apple waste. Scanning electron microscope, Fourier-transform infrared, X-ray diffractometer, and tensile strength analyses confirmed the formation of purified, porous, and heterogeneous densely packed multiple network polymers possessing cellulose properties. The water holding capacity (WHC) values of wet and dried BC membranes were 9179% and 226.9%, respectively, and the water absorption rate (WAR) of dry BC membranes was higher than that of wet membranes. Using BC as a tissue culture gelling agent, six genotypes from tomato and wheat seeds were cultured in vitro, for guaranteeing explant genetic diversity, over seven treatments. Treatment 5, included PLATIBACGEL as the main constituent, improved and sustained all in vitro seed germination, root penetration, and plant support. Likewise, repeated tomato micropropagation subcultures were successful. Results demonstrated applying PLATIBACGEL as a promising, reusable, cheap, and reliable alternative plant micropropagation media gelling agent. Wherefore, plant cellular developers and tissue-culturists can utilize bio-polymers like BC for better understanding plant cell response to different in vitro culturing conditions, with expected beneficial returns on gelling agents industry and markets as well. Graphical abstract

show abstract

Section: Mechanical Strengthsupporting

confidence: 88%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

View full text Add to dashboard Cite

show abstract

“…As a gelling agent, gellan gum has a lower mineral content, fewer impurities of organic substances [ 116 , 117 ], better water availability [ 118 ], and better facilitates the diffusion of inhibitive molecules such as phenols [ 119 ], compared to agar. Thus, it has advantageous ability to stimulate the growth and development of in vitro plants during micropropagation and microtuberization [ 120 ], and stimulate shoot multiplication [ 121 ], germination [ 122 ], conversion of polyembryoids into plantlets [ 123 ], callus induction and roots development [ 115 ]. A possible reason for agar’s inferiority compared to gellan gum lies in the immobilization of up to 30% MS medium salts in the gel [ 124 ].…”

Section: Strategy 2 Chemical Environment: Modifications Of Recovery M...mentioning

confidence: 99%

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Попова

Куличенко

Kim

2023

Biology

View full text Add to dashboard Cite

Cryopreservation is an effective option for the long-term conservation of plant genetic resources, including vegetatively propagated crops and ornamental plants, elite tree genotypes, threatened plant species with non-orthodox seeds or limited seed availability, as well as cell and root cultures useful for biotechnology. With increasing success, an arsenal of cryopreservation methods has been developed and applied to many species and material types. However, severe damage to plant material accumulating during the multi-step cryopreservation procedure often causes reduced survival and low regrowth, even when the optimized protocol is applied. The conditions at the recovery stage play a vital role in supporting material regrowth after cryopreservation and, when optimized, may shift the life-and-death balance toward a positive outcome. In this contribution, we provide an overview of the five main strategies available at the recovery stage to improve post-cryopreservation survival of in vitro plant materials and their further proliferation and development. In particular, we discuss the modification of the recovery medium composition (iron- and ammonium-free), exogenous additives to cope with oxidative stress and absorb toxic chemicals, and the modulation of medium osmotic potential. Special attention is paid to plant growth regulators used at various steps of the recovery process to induce the desired morphological response in cryopreserved tissues. Given studies on electron transport and energy provision in rewarmed materials, we discuss the effects of light-and-dark conditions and light quality. We hope that this summary provides a helpful guideline and a set of references for choosing the recovery conditions for plant species that have not been cryopreserved. We also propose that step-wise recovery may be most effective for materials sensitive to cryopreservation-induced osmotic and chemical stresses.

show abstract

“…laevigata callus induction was 7 g L − 1 . Furthermore, different kinds of gelling agents, such as gellan gum [26] and phytagel [23], can also result in differences in plant regeneration.…”

Section: Discussionmentioning

confidence: 99%

An efficient in vitro regeneration system via callus from hypocotyl and root of Iris laevigata

Li¹,

Fan²,

Fu³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Iris laevigata is an ornamental plant with strong cold resistance. However, its low reproductive capacity limits its landscape applications. The I. laevigata wild genetic resources also need to be protected. In order to develop an effective regeneration system, the optimum agar concentration for the induction medium was determined. Two explants (hypocotyl and root) were then cultured on medium containing different concentrations of plant growth regulator (PGR). In addition, three antibiotics were evaluated for controlling endophyte contamination. Results The highest induction rate (75.00%) was obtained from hypocotyl explants on Murashige and Skoog salt mixture (MS) medium containing 6-benzylaminopurine (6-BA) 0.5 mg L-1 + 2,4-Dichlorophenoxyacetic acid (2,4-D) 1.0 mg L-1 + 1-naphthylacetic acid (NAA) 0.4 mg L-1. The medium containing 6-BA 0.5 mg L-1 + 2,4-D 0.5 mg L-1 + NAA 0.2 mg L-1 achieved the greatest multiplication rate (73.33%). Media containing indole-3-butyric acid (IBA) 0.5 mg L-1 + 6-BA 1.5 mg L-1 + NAA 1.0 mg L-1 achieved the highest differentiation rate (39.72%) for hypocotyl induced calli. Medium containing 6-BA 2.0 mg L-1 + NAA 0.4 mg L-1 + kinetin (KT) 1.0 mg L-1 resulted in the highest differentiation rate (49.52%) for root induced calli. One hundred mg L-1 penicillin G resulted in the optimal rate for reducing endophyte contamination. Conclusions The research determined the optimum PGR concentrations for inducting and multiplying I. laevigata calli from hypocotyl and root explants and a satisfactory means for control endophyte contamination. This research will result in the efficient and reliable reproduction of I. laevigata for landscape applications, genetic development of new Iris varieties, and preservation of the wild genetic material.

show abstract

Effects of some gelling agents and their concentrations on conversion of oil palm polyembryoids into plantlets

Cited by 9 publications

References 14 publications

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

An efficient in vitro regeneration system via callus from hypocotyl and root of Iris laevigata

Contact Info

Product

Resources

About