Effects of some translation and transcription inhibitors on the development of the phenylalanine ammonia-lyase activity induced by light in radish cotyledons

Klein-Eude, Dominique; Rollin, P.; Huault, C.

doi:10.1016/0304-4211(74)90031-5

Cited by 10 publications

(5 citation statements)

References 14 publications

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“…Cotyledons from light and dark-grown radish (Raphanus sativus cultivar 'longue rave saumone'e') seedlings were cut off according to Klein-Eude et al [4]. Handling of the organs was as described previously [4].…”

Section: Methodsmentioning

confidence: 99%

“…Handling of the organs was as described previously [4]. Extraction was performed with 120 ml of 20 mM ammonium acetate buffer, pH 6, on 25 g fresh cotyledons during 15 min at 0°C.…”

Section: Methodsmentioning

confidence: 99%

“…A saccharose O-50% (w/v) was used. 20 mg of proteins, recovered from the ammonium hydroxide eluted peak in the affinity chromatography, were submitted to focusing at 5°C during 36 hr under an applied tension of 500 V. Recovery of the gradient, at the end of the experiment, was done as described in [6] and pH values (Taccusel Minisis type), enzyme assay [4], protein content [7], were measured on each 1 ml fraction.…”

Section: Methodsmentioning

confidence: 99%

“…Exposure of cotyledons to wavelengths maintaining sufficient amounts of P, (710-720 nm) are responsible for the highest increases in phenylalanine ammonia-lyase activity [2, 31. The observed effects of light can either reflect a de novo synthesis of active enzyme or the activation of a possible inactive precursor already present in darkgrown cotyledons [l]. Recently, in our laboratory, Klein-Eude et al [4], from the results of experiments conducted using cycloheximide as protein synthesis inhibitor, postulated the existence of an inactive form of the enzyme in dark-grown cotyledons.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for active and inactive forms of L‐phenylalanine ammonia‐lyase in etiolated and light‐grown radish cotyledons

et al. 1973

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Section: Methodsmentioning

confidence: 99%

“…Handling of the organs was as described previously [4]. Extraction was performed with 120 ml of 20 mM ammonium acetate buffer, pH 6, on 25 g fresh cotyledons during 15 min at 0°C.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evidence for active and inactive forms of L‐phenylalanine ammonia‐lyase in etiolated and light‐grown radish cotyledons

et al. 1973

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“…This suggests the production of a degradative enzyme about this time, which could well be a degradative enzyme for PAL. The presence of such an enzyme has been indicated in gherkin (10), pea (14), radish (16), and Xanthium (35). In all of these systems, the application of cycloheximide following a light exposure prevents the destruction of PAL, indicating that PAL inactivation requires protein synthesis.…”

Section: Resultsmentioning

confidence: 99%

Comparison of Three Phytochrome-mediated Processes in the Hypocotyl of Mustard

Kinnersley¹,

Davies²

1976

Plant Physiol.

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Anthocyanin synthesis, hair formation, and the synthesis of ascorbic acid oxidase are an phytochrome-medisted reactions occurring in the hypocotyl of mustard (Sinapis alba L.), controlled by phytochrome actully located in the hypocotyl. A comparison of these three reactions showed that in certain respects they differ greatly in their response to light. The ability of the seedling to respond to light by showing the three responses was strongly influenced by the state of development of the seedling. White light given very early after seed imbibition was unable to evoke any of the three reactions. By 50 hours after imbibition, all systems were fully inducible by light. The addition of actinomycin D to a fully competent seedling coincident with illumination strongly inhibited the development of all three responses. In contrast, the addition of cordycepin at this time inhibited the synthesis of anthocyanin and ascorbic acid oxidase but had no effect on hair formation. Cycdoheximide inhibited all three responses when given up to several hours after light.This suggests the necessity for RNA and protein synthesis for lightinduced expression of these reactions, and that the RNA species involved in the three reactions may have differing degrees of polyadenylation. The lag period between the onset of light and the first display of the response was 3 hours for anthocyanin and ascorbic acid oxidase synthesis, and about 5 hours for hair formation. Amounts of lght sufficient to give large increases in the levels of ascorbic acid oxidase and hair formation gave a much smaUler increase in anthocyania synthesis. Hair formation and ascorbic acid oxidase synthesis showed a much greater sensitivity to induction at early stages of seedling development than did anthocyanin synthesis. Following an inductive light period, anthocyanin synthesis was sensitive to far red light inhibition for a period twice as long as the other two reactions. The differences in the response of the three reactions to light sggest that the phytochrome-mediated reactions which control their development also differ.There has been much speculation on the mode of action of phytochrome (6,23,(25)(26)(27)32) particularly in regard to the nature of the primary reaction (25-27). Although various aspects of different phytochrome-controlled reactions have been compared (8,23,(25)(26)(27), no previous investigation has examined the influence of all of the parameters described in this study, on three different responses, occurring at the same time in the same organ. It is essential that such a comparison be conducted on reactions which occur in the same organ since different organs of a seedling respond differently to the formation of Pfr (23). Therefore, variations in the phytochrome control of various reactions in different organs could well reflect the nature of the organs involved or the stage of seedling development rather than the inductive processes per se.In the present study, the induction and development of three different reactions in the hypocotyl of ...

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Phytochrome-induced changes of ?-fructosidase activity in radish cotyledons

Zouaghi

1976

Planta

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β-fructofuranosidase (E.C.3.2.1.26) activity can hardly be detected in cotyledons from dark-grown radish (Raphanus sativus) seedlings. Under continuous far-red light two waves of β-fructofuranosidase activity can be observed. The first one concerns an acidic invertase which progressively disappears. This wave is followed by the appearance of a neutral invertase. Some characteristics of these two enzymes are given. Their regulation by light via phytochrome is discussed.

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Effects of some translation and transcription inhibitors on the development of the phenylalanine ammonia-lyase activity induced by light in radish cotyledons

Cited by 10 publications

References 14 publications

Evidence for active and inactive forms of L‐phenylalanine ammonia‐lyase in etiolated and light‐grown radish cotyledons

Evidence for active and inactive forms of L‐phenylalanine ammonia‐lyase in etiolated and light‐grown radish cotyledons

Comparison of Three Phytochrome-mediated Processes in the Hypocotyl of Mustard

Phytochrome-induced changes of ?-fructosidase activity in radish cotyledons

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