Effects of some β-carboline alkaloids on intact Trypanosoma cruzi epimastigotes

Rivas, Pilar Cebollero; Cassels, Bruce K.; Morello, Antonio; Repetto, Yolanda

doi:10.1016/s0742-8413(98)10069-5

Cited by 37 publications

(40 citation statements)

References 16 publications

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“…Hairy root cultures of Oxalis tuberose transformed with A. rhizogenes (ATCC-15834) showed better growth and exudation of harmine and harmaline in comparison to untransformed control roots. Harmine and Harmaline showed wide constitutive antimicrobial activity against soil-borne microorganisms (Bais et al 2003), anti-leishmanial activity (Di Giorgio et al 2004), bioinsecticidal activity (Rharrabe et al 2007), and cytotoxic to cancer cells (Rivas et al 1999). HPLC analysis of hairy roots clones of Hyoscyamus and Persian poppy of Papaver bracteatum L. showed that the relative concentration of alkaloids were virtually identical in wild-type and transgenic roots.…”

Section: Discussionmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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We have developed an efficient transformation system for Tribulus terrestris L., an important medicinal plant, using Agrobacterium rhizogenes strains AR15834 and GMI9534 to generate hairy roots. Hairy roots were formed directly from the cut edges of leaf explants 10-14 days after inoculation with the Agrobacterium with highest frequency transformation being 49 %, which was achieved using Agrobacterium rhizogenes AR15834 on hormone-free MS medium after 28 days inoculation. PCR analysis showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. Isolated transgenic hairy roots grew rapidly on MS medium supplemented with indole-3-butyric acid. They showed characteristics of transformed roots such as fast growth and high lateral branching in comparison with untransformed roots. Isolated control and transgenic hairy roots grown in liquid medium containing IBA were analyzed to detect ß-carboline alkaloids by High Performance Thin Layer Chromatograghy (HPTLC).Harmine content was estimated to be 1.7 μg g −1 of the dried weight of transgenic hairy root cultures at the end of 50 days of culturing. The transformed roots induced by AR15834 strain, spontaneously, dedifferentiated as callus on MS medium without hormone. Optimum callus induction and shoot regeneration of transformed roots in vitro was achieved on MS medium containing 0.4 mg L −1 naphthaleneacetic acid and 2 mg L −1 6-benzylaminopurine (BAP) after 50 days. The main objective of this investigation was to establish hairy roots in this plant by using A. rhizogenes to synthesize secondary products at levels comparable to the wild-type roots.

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Section: Discussionmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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“…The activity of a series of alkaloids against epimastigotes was associated to the inhibition of cell respiration, and the most active compound was apomorphine (Morello et al 1994). Using epimastigotes of Nif-resistant, the activity of β-carboline alkaloids was also associated with respiratory chain (Rivas et al 1999). Several glycoalkaloids were tested against epimastigotes, bloodstream and metacyclic trypomastigotes, and achaconine and a-solamargine showed higher activity than ketoconazole (Chataing et al 1998).…”

Section: Drugs Derived From Natural Sourcesmentioning

confidence: 99%

A Critical Review on Chagas Disease Chemotherapy

Coura¹,

Castro

2002

Mem. Inst. Oswaldo Cruz

846

444

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“…Some studies have shown that ␤-carboline compounds can induce mitochondrial dysfunction in trypanosomatids (10,11,35) , and consequently cellular damage (37,38).…”

Section: Fig 8 Cell Cycle Analysis Of Leishmania Amazonensis Promastimentioning

confidence: 99%

N -Butyl-[1-(4-Methoxy)Phenyl-9 H -β-Carboline]-3-Carboxamide Prevents Cytokinesis in Leishmania amazonensis

Stefanello

Panice

Ueda-Nakamura

et al. 2014

Antimicrob Agents Chemother

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dLeishmaniasis, a complex of diseases caused by protozoa of the genus Leishmania, is endemic in 98 countries, affecting approximately 12 million people worldwide. Current treatments for leishmaniasis have many disadvantages, such as toxicity, high costs, and prolonged treatment, making the development of new treatment alternatives highly relevant. Several studies have verified the antileishmanial activity of ␤-carboline compounds. In the present study, we investigated the in vitro antileishmanial activity of N-butyl-[1-(4-methoxy)phenyl-9H-␤-carboline]-3-carboxamide (␤-CB) against Leishmania amazonensis. The compound was active against promastigote, axenic amastigote, and intracellular amastigote forms of L. amazonensis, exhibiting high selectivity for the parasite. Moreover, ␤-CB did not exhibit hemolytic or mutagenic potential. Promastigotes treated with the alkaloid presented rounding of the body cell, cell membrane projections, an increase in the number of promastigotes presenting two flagella, and parasites of abnormal phenotype, with three or more flagella and/or nuclei. Furthermore, we observed an increase in the subpopulation of cells in the G 2 /M stage of the cell cycle. Altogether, these results suggest that ␤-CB likely prevents cytokinesis, although it does not interfere with the duplication of cell structures. We also verified an increase in O 2 ·؊ production and the accumulation of lipid storage bodies. Cell membrane integrity was maintained, in addition to the absence of phosphatidylserine externalization, DNA fragmentation, and autophagosomes. Although the possibility of an apoptotic process cannot be discarded, ␤-CB likely exerts its antileishmanial activity through a cytostatic effect, thus preventing cellular proliferation.

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Effects of some β-carboline alkaloids on intact Trypanosoma cruzi epimastigotes

Cited by 37 publications

References 16 publications

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

A Critical Review on Chagas Disease Chemotherapy

N -Butyl-[1-(4-Methoxy)Phenyl-9 H -β-Carboline]-3-Carboxamide Prevents Cytokinesis in Leishmania amazonensis

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