Effects of soy isoflavone genistein on orthodontic tooth movement in guinea pigs

Suparwitri, Sri; Pudyani, Pinandi Sri; Haryana, Sofia Mubarika; Agustina, Dewi

doi:10.20473/j.djmkg.v49.i3.p168-174

Cited by 8 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In adult individuals, the microarchitecture of trabecular bone becomes thin, while cortical bone becomes thin and porous 24 . A previous in vivo study demonstrated that in response to mechanical stimuli, the alveolar bone of the young rats was more active and sensitive than that of the adult rats 4 . As the aging process progresses, the periodontal ligament becomes more fibrotic and may influence how the tissues react to orthodontic forces 25 .…”

Section: Discussionmentioning

confidence: 94%

“…In general, results demonstrated that in the subjects receiving soybean isoflavone genistein, TGF-β1 levels were significantly higher than the other groups. A previous study found that genistein can significantly increase the number of osteoblasts during orthodontic tooth movement 4 . Soybeans are the most common source of isoflavones, which have Values are presented as mean ± standard deviation or p-value only.…”

Section: Discussionmentioning

confidence: 95%

“…Orthodontic treatment has become one of the most procedures in cosmetic dentistry; it is performed to improve malocclusion, achieve good occlusion and dentofacial harmony 2, 3 . The demand for orthodontic treatment has grown over time, not only in children, but also in adults 4 . The rising number of adults demanding orthodontic treatment presents a new challenge.…”

Section: Introductionmentioning

confidence: 99%

“…The effects of isoflavones on the improvement of osteoblast's proliferation have previously been observed. Genistein administration has shown potential to increase osteoblast numbers during OTM 4 . Further study to explore the effect of genistein in enhancing osteoblastogenesis through improving TGF-β1 level is needed to validate its advantage in orthodontic bone remodeling.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Different effects of soybean isoflavone genistein on transforming growth factor levels during orthodontic tooth movement among young and old rabbits

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background: Orthodontic treatment to improve aesthetics and for health reasons is performed in children and adults. Elderly individuals have low levels of estrogen, this results in alveolar bone resorption being greater than alveolar bone apposition. Isoflavones present in soybeans may be able to improve the remodeling process through the induction of osteoblastogenesis by increasing transforming growth factor-β1 (TGF-β1) levels. This study aimed to assess the comparative effect of soybean genistein isoflavone to TGF-β1 during orthodontic tooth movement among juvenile and adult rabbits. Methods: In this study, 12 healthy female rabbits were used. Subjects were divided into four groups (n=3); YG group (young rabbits), YGI group (young rabbits + isoflavones genistein), OG group (old rabbits), and OGI group (old rabbits + isoflavones genistein). Two lower incisors of the rabbit were moved distally using an orthodontic force (50 grams force) delivered by an open coil spring, which was inserted between two brackets. During active movements, the genistein isoflavones were given from the initial installation of the device until days 21, at a dose of 1.2 mg/kg BW once a day. Measurement of TGF-β levels were performed on days 1, 7, 14, 21 after appliance installation. TGF-β1 expression was analyzed using enzyme-linked immunosorbent assay (ELISA) and the optical density (OD) of the sample quantifed using a standard curve. The data obtained were analyzed using one-way Anova followed by Tukey HSD test. Results: The TGF-β1 levels were found to highest in the YGI group, and the TGF-β levels were significantly lower in the OG group (p<0.05). ELISA analysis also revealed that TGF-β1 levels of the OGI group were significantly higher when compared with the OG group (p<0.05). Conclusion: The administration of soybean genistein isoflavones could improve TGF-β1 levels in old rabbit’s during active orthodontic tooth movement.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Different effects of soybean isoflavone genistein on transforming growth factor levels during orthodontic tooth movement among young and old rabbits

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…At the next stage, the subjects were anesthetized with ketamine and orthodontic movement was set by installing the band matrix and orthodontic bracket on each mandibular incisor to enable it to move distally using an open coil spring (Ortho-tech, America) 15 with a power of 0.0525 N or 52.5 grams ( Figure 1). Observations were then made after the subjects had been sacrificed on Days 15 and 22 by extracting both their right and left mandibular incisors as well as periodic tissue and placing these in the fixative solution.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of orthodontic tooth movement by 3D micro-computed tomography (µ-CT) following caffeine administration

et al. 2019

View full text Add to dashboard Cite

Background: The compressive strength of orthodontic tooth movement will be distributed throughout the periodontal ligament and alveolar bone, resulting in bone resorption on the pressure side and new bone formation on the tension side. Caffeine, a member of the methyl xanthine family, represents a widely-consumed psychoactive substance that can stimulate osteoclastogenesis through an increase in RANKL. A 3D Micro-Computed Tomography (µ-CT) x-ray device can be used to measure orthodontic tooth movement and changes in periodontal ligament width. Purpose: The purpose of this research was to analyze the effects of caffeine on the distal movement distance of two mandibular incisors using 3D µ-CT. Methods: The research subjects (guinea pigs) were randomly divided into four groups. Of the two control groups created, one received two weeks of treatment and the other three weeks. The members of these two control groups were subjected to orthodontic movement but received no caffeine. Meanwhile, the other two groups were treatment groups whose members also received either two or three weeks of treatment. In these two treatment groups, the subjects were subjected to orthodontic movement and received a 6 mg/500 BM dose of caffeine. The orthodontic movement of the subjects was induced by installing a band matrix and orthodontic bracket on each mandibular incisor to move distally by means of an open coil spring. Observations were then conducted on days 15 and 22 with µ-CT x-rays to measure the distal movement distance of the two mandibular incisors and the width of the periodontal ligament. Results: The administration of caffeine increased the tooth movement on day 15 (p<0.05) and day 22 (p<0.05). The increase in the tooth movement on day 22 was greater than that on day 15 (p<0.05). The width of the periodontal ligament on the pressure side of the treatment groups experienced greater narrowing than that of the control groups (p<0.05). Meanwhile, the width of periodontal ligament on the tension side of the treatment groups widened more than that of the control groups (p<0.05). Conclusion: µ-CT x-ray can be used to evaluate the extent of orthodontic movement in addition to the width of the mandibular incisor periodontal ligament during orthodontic tooth movement. Moreover, it has been established that the administering of caffeine can improve orthodontic tooth movement.

show abstract

Effect of olive oil administration on the level of transforming growth factor β1 during orthodontic tooth movement in old and young guinea pigs

Suparwitri

Noviasari

2019

F1000Res

Self Cite

View full text Add to dashboard Cite

Background: Orthodontic tooth movement occurs due to continuous pressure on the teeth, causing the remodeling of the alveolar bone. The tissue will respond to bone growth factors, such as transforming growth factor β1 (TGF-β1), fibroblasts, and bone morphogenetic proteins, for new bone formation. The remodeling process is balanced in young adults, but there is an imbalance in older individuals due to decreased differentiation, activity, and life span of osteoblasts, and increased osteoclasts. Olive oil contains lots of antioxidants and can slow down the aging process. This study aims to study the differences in TGF-β1 levels between old and young guinea pigs, and the difference between olive oil administration on TGF-β1 levels in old and young guinea pigs with orthodontic tooth movement. Methods: 12 guinea pigs divided into 4 groups: young guinea pigs (4-5 months) not given olive oil; young guinea pigs given olive oil; old guinea pigs (30-31 months) given olive oil; old guinea pigs given olive oil. The teeth were moved using an open coil spring mounted on the brackets on both lower incisors. Gingival sulcus fluid samples were taken on days 0, 7 and 14 of the movement of the teeth. TGF-β1 levels were analyzed using ELISA. Results: Three-way ANOVA and post hoc statistical tests showed that TGF-β1 levels in young guinea pigs were significantly higher than old guinea pigs on days 0, 7 and 14 (p<0.05). TGF-β1 levels in both young and old guinea pigs who were given olive oil was significantly higher than those not given olive oil on days 0, 7 and 14 (p<0.05). Conclusions: TGF-β1 levels in the younger age guinea pigs were higher than the older age, and olive oil could increase TGF-β1 levels in the older age guinea pigs.

show abstract

Effects of soy isoflavone genistein on orthodontic tooth movement in guinea pigs

Abstract: Background: Osteoblast and osteoclast are the important factor in periodontal tissue remodeling for the orthodontic treatment success. Resorption process takes place in compression area by osteoclast and apposition in the tension

Cited by 8 publications

References 20 publications

Different effects of soybean isoflavone genistein on transforming growth factor levels during orthodontic tooth movement among young and old rabbits

Different effects of soybean isoflavone genistein on transforming growth factor levels during orthodontic tooth movement among young and old rabbits

Evaluation of orthodontic tooth movement by 3D micro-computed tomography (µ-CT) following caffeine administration

Effect of olive oil administration on the level of transforming growth factor β1 during orthodontic tooth movement in old and young guinea pigs

Contact Info

Product

Resources

About