Effects of spark dielectric barrier discharge plasma on water sterilization and seed germination

Dhakal, Oat Bahadur; Dahal, Ram Kumar; Acharya, Tirtha Raj; Lamichhane, Prajwal; Gautam, Sanjeev K.; Lama, Bhupendra; Khanal, Raju; Kaushik, N. K.; Choi, Eun Ha; Chalise, Roshan

doi:10.1016/j.cap.2023.08.006

Cited by 24 publications

(6 citation statements)

References 52 publications

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“…x concentrations. In some cases [17,31], the results of our method showed significant deviation from the values reported. These differences can be attributed to a lack of temperature correction, different measurement moments and additional ionic species unaccounted for with our method.…”

Section: Ph and Conductivity Methods With Previously Published Workcontrasting

confidence: 75%

Assessment of reactive species concentrations in plasma-treated water: a pH and conductivity-based method validated by absorption spectroscopy

Petković,

van de Wege,

Wubs

et al. 2024

J. Phys. D: Appl. Phys.

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To assess the species in plasma-treated water, two methods were compared: calibrated absorption spectroscopy and a method using pH and conductivity to determine the amount of dominant reactive species in the water. A function fitting method was developed for the analysis of the absorption spectra based on solutions with known concentrations. The influence of plasma treatment time on demineralized water and the NOx concentration was studied using the two methods; the results in both cases show an almost linear growth with time. The method, utilizing pH and conductivity, shows consistent results with those achieved using UV absorption spectroscopy in considerably less time and in a more cost-efficient manner.

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Section: Ph and Conductivity Methods With Previously Published Workcontrasting

confidence: 75%

Assessment of reactive species concentrations in plasma-treated water: a pH and conductivity-based method validated by absorption spectroscopy

Petković,

van de Wege,

Wubs

et al. 2024

J. Phys. D: Appl. Phys.

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“…Additionally, the vibrational temperature (Tv) and rotational temperature (Tg) of the cold soft jet plasma were computed at 0.7 eV and 742 K, respectively. These elevated electrons, vibrational, and gas temperatures, in comparison to other plasma devices, establish the soft jet plasma as a proli c generator of an abundant quantity of reactive species, with a notable emphasis on NO production [30,35].…”

Section: Resultsmentioning

confidence: 99%

Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines

Giacomo,

Balaha,

Pinti

et al. 2023

Preprint

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Objective: Cold atmospheric plasma (CAP) is a novel therapeutic approach for cancer treatment. It can be used to treat liquids - plasma-activated media (PAM) - which are then transferred to the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM and assessing their in vitro selectivity against head and neck cancer cell lines (HNC). Materials and methods: PAM were obtained by exposing 2 and 5 mL of medium to CAP for 5, 10 and 20 minutes at a 6 mm working distance. Anions kinetics was evaluated by ion chromatography. In addition, inhibition of cell proliferation by MTS assay, apoptosis occurrence and cell cycle modifications by flow cytometry were assessed on primary human gingival fibroblasts (hGF) and the HNC cell lines HSC2, HSC4 and A253. Results: All the 2 mL conditions showed a significant reduction in cell proliferation whereas for the 5 mL the effect was milder, but the time-dependence was more evident. In addition, hGF were unaffected by the 5 mL PAM, indicating a selectivity for cancer cells. Conclusions: The media chemical composition modified by CAP exposure influenced cell proliferation by modulating cell cycle and inducing apoptosis in cancer cells, without affecting normal cells. Clinical Relevance: The present investigation represents a starting point to favour the clinical translation of CAP as a precision medicine tool by proposing an innovative method, namely ion chromatography, to standardize the quantification of plasma-derived RONS and proving its selectivity in inactivating tumor cells over non-malignant cells. These strategies could be applied to identify the optimal parameter configuration to achieve the desired treatment/therapeutic outcome and to aid the definition of clinical protocols.

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“…The rising electrical conductivity and ORP in liquids treated with air CAP can be attributed to the ionization of molecules and the generation of ROS and RNS 45 . As the air CAP treatment progresses, these processes lead to the production of ions and oxidative species, resulting in increased electrical conductivity and the establishment of a more oxidative environment with a higher ORP.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of direct air gas soft jet plasma for the in vitro reduction of oral bacterial biofilms

Puca,

Marinacci,

Pinti

et al. 2024

Sci Rep

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The aim of this study was to evaluate the antimicrobial efficacy of an air gas soft jet CAP for its potential use in removing oral biofilms, given that plasma-based technologies have emerged as promising methods in periodontology. Two types of biofilms were developed, one by Streptococcus mutans UA 159 bacterial strain and the other by a complex mixture of saliva microorganisms isolated from a patient with periodontitis. This latter biofilm was characterized via Next Generation Sequencing to determine the main bacterial phyla. The CAP source was applied at a distance of 6 mm for different time points. A statistically significant reduction of both CFU count and XTT was already detected after 60 s of CAP treatment. CLSM analysis supported CAP effectiveness in killing the microorganisms inside the biofilm and in reducing the thickness of the biofilm matrix. Cytotoxicity tests demonstrated the possible use of CAP without important side effects towards human gingival fibroblasts cell line. The current study showed that CAP treatment was able to significantly reduce preformed biofilms developed by both S. mutans and microorganisms isolated by a saliva sample. Further studies should be conducted on biofilms developed by additional saliva donors to support the potential of this innovative strategy to counteract oral pathogens responsible for periodontal diseases.

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Effects of spark dielectric barrier discharge plasma on water sterilization and seed germination

Cited by 24 publications

References 52 publications

Assessment of reactive species concentrations in plasma-treated water: a pH and conductivity-based method validated by absorption spectroscopy

Assessment of reactive species concentrations in plasma-treated water: a pH and conductivity-based method validated by absorption spectroscopy

Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines

Antimicrobial efficacy of direct air gas soft jet plasma for the in vitro reduction of oral bacterial biofilms

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