Effects of Spatial Structure and Reduced Growth Rates on Evolution in Bacterial Populations

Ridenhour, Ben J.; Forney, Larry J.

doi:10.1007/978-3-319-69078-0_8

Cited by 6 publications

(3 citation statements)

References 152 publications

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“…Due to spatial structure, bacteria that grow in biofilms only compete locally with their neighbors, which protracts selective sweeps and leads to increased genotypic and phenotypic variation within a population [29][30][31][32][33][34][35][36][37][38][39] . Additionally, the gradients of nutrients and electron acceptors cause habitat heterogeneity that results in local adaptation of subpopulations.…”

Section: Introductionmentioning

confidence: 99%

Biofilms preserve the transmissibility of a multi-drug resistance plasmid

Metzger

Ridenhour

France

et al. 2022

npj Biofilms Microbiomes

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Self-transmissible multidrug resistance (MDR) plasmids are a major health concern because they can spread antibiotic resistance to pathogens. Even though most pathogens form biofilms, little is known about how MDR plasmids persist and evolve in biofilms. We hypothesize that (i) biofilms act as refugia of MDR plasmids by retaining them in the absence of antibiotics longer than well-mixed planktonic populations and that (ii) the evolutionary trajectories that account for the improvement of plasmid persistence over time differ between biofilms and planktonic populations. In this study, we evolved Acinetobacter baumannii with an MDR plasmid in biofilm and planktonic populations with and without antibiotic selection. In the absence of selection, biofilm populations were better able to maintain the MDR plasmid than planktonic populations. In planktonic populations, plasmid persistence improved rapidly but was accompanied by a loss of genes required for the horizontal transfer of plasmids. In contrast, in biofilms, most plasmids retained their transfer genes, but on average, plasmid, persistence improved less over time. Our results showed that biofilms can act as refugia of MDR plasmids and favor the horizontal mode of plasmid transfer, which has important implications for the spread of MDR.

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Section: Introductionmentioning

confidence: 99%

Biofilms preserve the transmissibility of a multi-drug resistance plasmid

Metzger

Ridenhour

France

et al. 2022

npj Biofilms Microbiomes

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“…Empirical work has considered the impact of spatial structure through habitat fragmentation on trait evolution ( Cheptou et al, 2017 ; Urban et al, 2008 ), how population subdivision modulates the extent of adaptive change ( Bailey et al, 2021 ; Baym et al, 2016 ; Chao & Levin, 1981 ; Habets et al, 2006 , 2007 ; Korona et al, 1994 ; Kryazhimskiy et al, 2012 ; Miralles et al, 1999 ; Nahum et al, 2015 ; Perfeito et al, 2007 ; Zhang et al, 2011 ) and community resilience ( Limdi et al, 2018 ). Other work in microbiology has examined the emergence and fate of diversity in spatially structured environments associated with colony growth or biofilms ( Borer et al, 2020 ; Celik Ozgen et al, 2018 ; France et al, 2018 , 2019 ; Kerr et al, 2002 ; Nadell et al, 2010 , 2016 ; Steenackers et al, 2016 ; Trubenová et al, 2022 ) but lacks explicit descriptions of spatial structure or conflates it with variation in conditions of growth that generate divergent selection ( Chen & Kassen, 2020 ; Leale & Kassen, 2018 ). Missingare explicit tests of theory on how the spatial arrangement of populations in space impacts the dynamics of adaptive evolution, including the rate of spread of a beneficial mutation and the probability of fixation.…”

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confidence: 99%

Experimental evidence that network topology can accelerate the spread of beneficial mutations

Chakraborty,

Nemzer,

Kassen

2023

Evolution Letters

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Whether and how the spatial arrangement of a population influences adaptive evolution has puzzled evolutionary biologists. Theoretical models make conflicting predictions about the probability that a beneficial mutation will become fixed in a population for certain topologies like stars, in which “leaf” populations are connected through a central “hub.” To date, these predictions have not been evaluated under realistic experimental conditions. Here, we test the prediction that topology can change the dynamics of fixation both in vitro and in silico by tracking the frequency of a beneficial mutant under positive selection as it spreads through networks of different topologies. Our results provide empirical support that meta-population topology can increase the likelihood that a beneficial mutation spreads, broaden the conditions under which this phenomenon is thought to occur, and points the way toward using network topology to amplify the effects of weakly favored mutations under directed evolution in industrial applications.

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“…Although growth rates and carrying capacities are historically used as part of definitions of “fitness” in the laboratory, real world fitness of microbes is often weakly associated with these parameters (Concepción-Acevedo et al 2015 , Momeni et al 2017 , Bansept et al 2021 ). Instead, fitness in a spatially structured environment with biotic interactions is more often a function of physical and social interactions, where spatial scales and interaction structures are critical aspects of selection (Hansen et al 2007 , France et al 2018 , Liu et al 2019 , Gorter et al 2020 , Conwill et al 2022 , Hoces et al 2022 ). Investigations of realistically structured populations, where temporal and spatial dynamics are considered explicitly, are important for understanding microbial evolution and intraspecies diversity.…”

Section: Discussionmentioning

confidence: 99%

Parallel evolution of alternate morphotypes of Chryseobacterium gleum during experimental evolution with Caenorhabditis elegans

Duckett,

Taylor,

Bowman

et al. 2024

FEMS Microbiology Ecology

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Microbial evolution within polymicrobial communities is a complex process. Here we report within-species diversification within multi-species microbial communities during experimental evolution with the nematode Caenorhabditis elegans. We describe morphological diversity in the target species Chryseobacterium gleum, which developed a novel colony morphotype in a small number of replicate communities. Alternate morphotypes co-existed with original morphotypes in communities, as well as in single-species experiments using evolved isolates. We found that the original and alternate morphotypes differed in motility and in spatial expansion in the presence of C. elegans. This study provides insight into the emergence and maintenance of intra-species diversity in the context of microbial communities.

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Effects of Spatial Structure and Reduced Growth Rates on Evolution in Bacterial Populations

Cited by 6 publications

References 152 publications

Biofilms preserve the transmissibility of a multi-drug resistance plasmid

Biofilms preserve the transmissibility of a multi-drug resistance plasmid

Experimental evidence that network topology can accelerate the spread of beneficial mutations

Parallel evolution of alternate morphotypes of Chryseobacterium gleum during experimental evolution with Caenorhabditis elegans

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