Effects of sphingosylphosphorylcholine against oxidative stress and acute lung ınjury ınduced by pulmonary contusion in rats

Aksu, Burhan; Ayvaz, Süleyman; Aksu, Feyza; Karaca, Turan; Cemek, Mustafa; Ayaz, Ahmet; Demirtaş, Selim

doi:10.1016/j.jpedsurg.2014.06.007

Cited by 11 publications

(9 citation statements)

References 42 publications

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“…The effect of high-dose SPC may be dependent on the triphasic effects of the dose. 23 But in this study, we did not see the triphasic effects in 2 and 10 lM SPC dosages. These two separate doses gave the similar effects, the possible reasons for this similarity may be the insufficiency of renal tissue concentration for showing triphasic effects or the difference in answers between lung and kidneys to high concentrations of SPC.…”

Section: Discussioncontrasting

confidence: 61%

“…We have previously shown used SPC can reduce the hepatic damage in extrahepatic cholestasis and lung contusion by prevention of the oxidative stress and of the inflammatory process in liver bile duct ligation and pulmonary contusion. 17,23 The contrary results suggest that the SPC-induced generation of ROS plays a crucial role in the cell death of endothelial cells through extracellular signal-regulated kinase-dependent pathways. 31 In this study, the anti-oxidant property of SPC re-expressed using biochemical methods.…”

Section: Discussionmentioning

confidence: 99%

“…34,47 Sphingolipids are novel second messengers mediating a variety of cellular responses, including cell proliferation and apoptosis. 23 Previous studies have shown that high concentrations of SPC induce apoptosis via the mitochondrial pathway. 31,32 The similar cell culture study using low-dose SPC concentration demonstrated that SPC was effective as an anti-apoptotic agent.…”

Section: Discussionmentioning

confidence: 99%

“…The avidin biotin complex method was used for immunohistochemical staining, as previously reported. 23 The sections were incubated with rabbit polyclonal anti-iNOS (Abcam, dilution 1:100) (Abcam, Cambridge, MA). Diaminobenzidine (DAB) was used as a chromogen, and the sections were counterstained with hematoxylin.…”

Section: Inducible Nitric Oxide Synthase (Inos) Immunohistochemistrymentioning

confidence: 99%

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Antioxidant and renoprotective effects of sphingosylphosphorylcholine on contrast-induced nephropathy in rats

Aksu

Unlu

et al. 2016

Renal Failure

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Contrast induced nephropathy (CIN) is a major cause of morbidity, and increased costs as well as an increased risk of death. This study was evaluated effects of exogenous sphingosylphosphorylcholine (SPC) administration on CIN in rats. Eight animals were included in each of the following eight groups: control, control phosphate-buffered solution (PBS), control SPC 2, control SPC 10, CIN, CIN PBS, CIN SPC 2 and CIN SPC 10. The induced nephropathy was created by injected with 4 g iodine/kg body weight. SPC was administered 3 d at a daily two different doses of 2 μm/mL and 10 μm/mL intraperitoneally. The severity of renal injury score was determined by the histological and immunohistochemical changes in the kidney. Malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) were determined to evaluate the oxidative status in the renal tissue. Treatment with 2 and 10 μM SPC inhibited the increase in renal MDA, NO levels significantly and also attenuated the depletion of SOD in the renal injuryCIN. These data were supported by histopathological findings. The inducible nitric oxide synthase positive cells and apoptotic cells in the renal tissue were observed to be reduced with the 2 and 10 μM SPC treatment. These findings suggested that 2 and 10 μM doses can attenuate renal damage in contrast nephropathy by prevention of oxidative stress and apoptosis. The low and high dose SPC may be a promising new therapeutic agent for CIN.

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Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Inducible Nitric Oxide Synthase (Inos) Immunohistochemistrymentioning

confidence: 99%

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Antioxidant and renoprotective effects of sphingosylphosphorylcholine on contrast-induced nephropathy in rats

Aksu

Unlu

et al. 2016

Renal Failure

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“…Excessive ROS should be eliminated from cells by antioxidant‐dependent defense mechanism that counter balances excessive ROS generation using entities, such as, SOD‐1, SOD‐2, GPx1 and CAT. When unchecked, increased ROS production and decreased antioxidant activities can trigger oxidative stress (Aksu et al, ). In the present study, WGP pretreatment was found to increase the expressions of all of these antioxidant markers significantly as compared with LPS‐challenged mice.…”

Section: Discussionmentioning

confidence: 99%

Wheatgrass‐Derived Polysaccharide Has Antiinflammatory, Anti‐Oxidative and Anti‐Apoptotic Effects on LPS‐Induced Hepatic Injury in Mice

Nepali

Lee

et al. 2017

Phytotherapy Research

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Hepatic injury occurs frequently during sepsis, and polysaccharides isolated from plants have been reported to have antiinflammatory and antioxidant effects in various models. However, the effect of wheatgrass-derived polysaccharide (WGP) has not been previously studied. In the present study, we investigated the effect of WGP on lipopolysaccharide (LPS)-induced hepatic injury in mice. Mice were pre-treated with WGP (100 or 200 mg/kg daily for 2 days) and then challenged with LPS (1 mg/kg, intraperitoneal), and sacrificed after 12 h. Wheatgrass-derived polysaccharide decreased serum aminotransferase levels and histological changes as compared with LPS-challenged mice. Wheatgrass-derived polysaccharide also significantly inhibited LPS-induced pro-inflammatory cytokine up-regulation and improved the oxidative status of liver tissues. Furthermore, these effects were found to be mediated by the suppression of the transcriptional activity of nuclear factor-kappa B (NF-κB), due to inhibitions of transforming growth factor beta (TGF-β)-activated kinase (TAK)-1 phosphorylation and inhibition of kappa B (IκB)-α degradation. In addition, WGP inhibited the activations of mitogen-activated protein kinases (MAPKs). Wheatgrass-derived polysaccharide also attenuated hepatic cell death by modulating caspase-3 and apoptosis associated mitochondrial proteins, such as, B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax). Taken together, WGP possesses antiinflammatory, anti-oxidant and anti-apoptotic activity and ameliorates LPS-induced liver injury in mice. Copyright © 2017 John Wiley & Sons, Ltd.

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Sphingosylphosphorylcholine alleviates hypoxia-caused apoptosis in cardiac myofibroblasts via CaM/p38/STAT3 pathway

Liu

Yang

et al. 2020

Apoptosis

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Effects of sphingosylphosphorylcholine against oxidative stress and acute lung ınjury ınduced by pulmonary contusion in rats

Cited by 11 publications

References 42 publications

Antioxidant and renoprotective effects of sphingosylphosphorylcholine on contrast-induced nephropathy in rats

Antioxidant and renoprotective effects of sphingosylphosphorylcholine on contrast-induced nephropathy in rats

Wheatgrass‐Derived Polysaccharide Has Antiinflammatory, Anti‐Oxidative and Anti‐Apoptotic Effects on LPS‐Induced Hepatic Injury in Mice

Sphingosylphosphorylcholine alleviates hypoxia-caused apoptosis in cardiac myofibroblasts via CaM/p38/STAT3 pathway

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