Effects of sponge-derived polybrominated diphenyl ethers on human cancer cell α-<i>N</i>-acetylgalactosaminidase and bacterial α-<scp>d</scp>-galactosidase and their antioxidant activity

Utkina, N. K.; Ermakova, Svetlana P.; Bakunina, I. Yu.

doi:10.1039/d2va00269h

Cited by 1 publication

(1 citation statement)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HTB-72 TM ), as well as the murine healthy epidermal cell line JB6 Cl 41 (ATCC #no. CRL-2010™) [ 42 ]. At present, there is very little information concerning the inhibitors of GH27 family α-N-acetylgalactosaminidase.…”

Section: Discussionmentioning

confidence: 99%

Effect of Phlorotannins from Brown Algae Costaria costata on α-N-Acetylgalactosaminidase Produced by Duodenal Adenocarcinoma and Melanoma Cells

et al. 2022

View full text Add to dashboard Cite

The inhibitor of human α-N-acetylgalactosaminidase (α-NaGalase) was isolated from a water–ethanol extract of the brown algae Costaria costata. Currently, tumor α-NaGalase is considered to be a therapeutic target in the treatment of cancer. According to NMR spectroscopy and mass spectrometric analysis, it is a high-molecular-weight fraction of phlorethols with a degree of polymerization (DP) equaling 11–23 phloroglucinols (CcPh). It was shown that CcPh is a direct inhibitor of α-NaGalases isolated from HuTu 80 and SK-MEL-28 cells (IC50 0.14 ± 0.008 and 0.12 ± 0.004 mg/mL, respectively) and reduces the activity of this enzyme in HuTu 80 and SK-MEL-28 cells up to 50% at concentrations of 15.2 ± 9.5 and 5.7 ± 1.6 μg/mL, respectively. Molecular docking of the putative DP-15 oligophlorethol (P15OPh) and heptaphlorethol (PHPh) with human α-NaGalase (PDB ID 4DO4) showed that this compound forms a complex and interacts directly with the Asp 156 and Asp 217 catalytic residues of the enzyme in question. Thus, brown algae phlorethol CcPh is an effective marine-based natural inhibitor of the α-NaGalase of cancer cells and, therefore, has high therapeutic potential.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Phlorotannins from Brown Algae Costaria costata on α-N-Acetylgalactosaminidase Produced by Duodenal Adenocarcinoma and Melanoma Cells

et al. 2022

View full text Add to dashboard Cite

show abstract

Effects of sponge-derived polybrominated diphenyl ethers on human cancer cell α-N-acetylgalactosaminidase and bacterial α-d-galactosidase and their antioxidant activity

Abstract: During the search for glycosidase inhibitors among marine natural products that are environmental contaminants, we evaluated for the first time the potency of polybrominated diphenyl ethers 1-7 (5: OH-BDE47, 6:...

Cited by 1 publication

References 70 publications

Effect of Phlorotannins from Brown Algae Costaria costata on α-N-Acetylgalactosaminidase Produced by Duodenal Adenocarcinoma and Melanoma Cells

Effect of Phlorotannins from Brown Algae Costaria costata on α-N-Acetylgalactosaminidase Produced by Duodenal Adenocarcinoma and Melanoma Cells

Contact Info

Product

Resources

About