Effects of Srxn1 on growth and Notch signalling of astrocyte induced by hydrogen peroxide

Li, Lan; Lin, Guangjun; Gu, Huizi; Yu, Lei; Ni, Changwei

doi:10.1080/21691401.2019.1614016

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…32) According to reports, the expression level of Srxn1 was increased when cells were stimulated by external injury. 33,34) In this study, we used PDGF-BB to treat MOVAS cells and found that treatment of PDGF-BB increased the mRNA and protein expression of Srxn1 in a dose-and time-dependent manner, which was consistent with previous studies. PDGF interacts with PDGF receptor (PDGFR) on cell membrane inducing dimerization and the phosphorylation of PDGFR can activate several downstream signaling pathways such as Ras/Rac, MAPK, PI3 K, STAT, and Src, which is essential for the regulation of cellular function.…”

Section: Discussionsupporting

confidence: 89%

Sulfiredoxin-1 Inhibits PDGF-BB-Induced Vascular Smooth Muscle Cell Proliferation and Migration by Enhancing the Activation of Nrf2/ARE Signaling

et al. 2022

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Sulfiredoxin1 (Srxn1), an endogenous antioxidant protein, is involved in cardiovascular diseases. In this study, we aimed to investigate the role of Srxn1 in VSMCs and its molecular mechanism. The murine vascular smooth muscle cells MOVAS were treated with different doses of platelet-derived growth factor-BB (PDGF-BB); then, Srxn1 expression was detected using reverse transcription-quantitative polymerase chain reaction and western blot analysis. MTT and wound healing assay were used to examine the effect of Srxn1 on MOVAS cell proliferation and migration. Reactive oxygen species (ROS) production, malondialdehyde (MDA) level, and superoxide dismutase (SOD) activity in MOVAS cells were detected using corresponding commercial kits. Moreover, the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase 2 (MMP-2), and nuclear factor erythroid-2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling-related proteins was detected using western blot analysis. In our study, PDGF-BB dose-dependently increased Srxn1 expression in MOVAS cells, and Srxn1 expression was increased with time dependence in PDGF-BB-treated MOVAS cells. The knockdown of Srxn1 increased PDGF-BB-induced the proliferation, migration, ROS production, MDA level, and the protein expression of PCNA and MMP-2, as well as decreased SOD activity and the expression of Nrf2/ARE signaling-related proteins in PDGF-BB-stimulated MOVAS cells. However, the overexpression of Srxn1 showed the opposite results to those of knockdown of Srxn1. Moreover, the inhibitory effects of Srxn1 overexpression on PDGF-BB induced proliferation, migration, ROS production, and MDA level and the promotion of Srxn1 overexpression on PDGF-BB induced SOD activity were partially reversed by the knockdown of Nrf2. Srxn1 inhibited PDGF-BB-induced proliferation, migration, and oxidative stress through activating Nrf2/ ARE signaling.

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Section: Discussionsupporting

confidence: 89%

Sulfiredoxin-1 Inhibits PDGF-BB-Induced Vascular Smooth Muscle Cell Proliferation and Migration by Enhancing the Activation of Nrf2/ARE Signaling

et al. 2022

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“…SRXN1 , an endogenous antioxidant, has been shown to protect against exogenous compound-induced oxidative stress in vitro and in vivo ( 42 , 43 ). However, numerous studies reported that the anti-oxidative stress effect of SRXN1 could not protect against pulmonary disease ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of SRXN1 and KRT6A as Key Genes in Smoking-Related Non-Small-Cell Lung Cancer Through Bioinformatics and Functional Analyses

Zhou

Jiang

et al. 2022

Front. Oncol.

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BackgroundLung cancer is the leading cause of cancer-related mortality worldwide. Although cigarette smoking is an established risk factor for lung cancer, few reliable smoking-related biomarkers for non-small-cell lung cancer (NSCLC) are available. An improved understanding of these biomarkers would further the development of new biomarker-targeted therapies and lead to improvements in overall patient survival.MethodsWe performed bioinformatic analysis to screened potential target genes, then quantitative PCR, western, siRNA, CCK-8, flow cytometry, tumorigenicity assays in nude mice were performed to validated the function.ResultsIn this study, we identified 83 smoking-related genes (SRGs) based on an integration analysis of two Gene Expression Omnibus (GEO) datasets, and 27 hub SRGs with potential carcinogenic effects by analyzing a dataset of smokers with NSCLC in The Cancer Genome Atlas (TCGA) database. A survival analysis revealed three genes with potential prognostic value, namely SRXN1, KRT6A and JAKMIP3. A univariate Cox analysis revealed significant associations of elevated SRXN1 and KRT6A expression with prognosis. A receiver operating characteristic (ROC) curve analysis indicated the high diagnostic value of SRXN1 and KRT6A for smoking and cancer. Quantitative PCR and western blotting validated the increased expression of SRXN1 and KRT6A mRNA and protein, respectively, in lung cancer cell lines and NSCLC tissues. In patients with NSCLC, SRXN1 and KRT6A expression was associated with the tumor–node–metastasis (TNM) stage, presence of metastasis, history of smoking and daily smoking consumption. Furthermore, inhibition of SRXN1 or KRT6A suppressed viability and enhanced apoptosis in the A549 human lung carcinoma cell line. Tumorigenicity assays in nude mice confirmed that the siRNA-mediated downregulation of SRXN1 and KRT6A expression inhibited tumor growth in vivo.ConclusionsIn summary, SRXN1 and KRT6A act as oncogenes in NSCLC and might be potential biomarkers of smoking exposure and the early diagnosis and prognosis of NSCLC in smokers, which is vital for lung cancer therapy.

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“…POD activity was measured using the protocols described by Sharma et al [47]. SOD activity was measured following the methods used previously [48]. CAT activity was measured following Han et al [49].…”

Section: Determination Of Related Physiological Indexesmentioning

confidence: 99%

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

Han

Zhou

et al. 2020

IJMS

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NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in Malus baccata (L.) Borkh. In this study, the transcription factor MbNAC25 in M. baccata was isolated as a member of the plant-specific NAC family that regulates stress responses. Expression of MbNAC25 was induced by abiotic stresses such as drought, cold, high salinity and heat. The ORF of MbNAC25 is 1122 bp, encodes 373 amino acids and subcellular localization showed that MbNAC25 protein was localized in the nucleus. In addition, MbNAC25 was highly expressed in new leaves and stems using real-time PCR. To analyze the function of MbNAC25 in plants, we generated transgenic Arabidopsis plants that overexpressed MbNAC25. Under low-temperature stress (4 • C) and high-salt stress (200 mM NaCl), plants overexpressing MbNAC25 enhanced tolerance against cold and drought salinity conferring a higher survival rate than that of wild-type (WT). Correspondingly, the chlorophyll content, proline content, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased, while malondialdehyde (MDA) content was lower. These results indicated that the overexpression of MbNAC25 in Arabidopsis plants improved the tolerance to cold and salinity stress via enhanced scavenging capability of reactive oxygen species (ROS). The proteins encoded by NAC family genes form homodimers or heterodimers at the N-terminal, consist of about 150 amino acids. The N-terminal regions of NAC proteins share a conserved DNA binding region, which is divided into five subdomains (A-E) [8], whereas the C-terminal region that contains a transcriptional regulatory domain is highly diversified.The regulatory mechanism of NAC TFs consists of two types. The first one is the regulation of transcriptional levels including phosphorylation and ubiquitination miRNAs, which can regulate the expression of TFs at the protein level. The other is that post-transcriptional regulation can be carried out by binding to the target mRNA. NAC transcription factors can regulate target genes by binding to NACRS (NAC recognition sequence) or NACBS (NAC binding sequence), thereby affecting fruit appearance, ripeness, aging, flavor and nutritional quality [9,10]. In addition, NAC transcription factors also bind DNA or other protein kinases to regulate growth and development in plants and various physiological processes, including top meristem [11], secondary wall formation [12], flower organ development [13], bud differentiation [14], embryo development [15], lateral root formation [16,17], plant organ aging and fruit maturity [18].Abiotic stress factors such as drought, high salt, cold and low temperature seriously affect the growth of plants. Under these stresses, a large number of reactive oxygen species will be gener...

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Effects of Srxn1 on growth and Notch signalling of astrocyte induced by hydrogen peroxide

Cited by 14 publications

References 22 publications

Sulfiredoxin-1 Inhibits PDGF-BB-Induced Vascular Smooth Muscle Cell Proliferation and Migration by Enhancing the Activation of Nrf2/ARE Signaling

Sulfiredoxin-1 Inhibits PDGF-BB-Induced Vascular Smooth Muscle Cell Proliferation and Migration by Enhancing the Activation of Nrf2/ARE Signaling

Identification of SRXN1 and KRT6A as Key Genes in Smoking-Related Non-Small-Cell Lung Cancer Through Bioinformatics and Functional Analyses

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

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