Effects of stachyose on the intestinal microbiota and barrier in antibiotic-treated mice

Xi, Menglu; Zhao, Shenglong; Ge, Wupeng; Chen, Ying; Cui, Xiuxiu; Sun, Qiwu

doi:10.1016/j.jff.2021.104493

Cited by 7 publications

(3 citation statements)

References 23 publications

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“…In the antianxiety test, Stachyose + PS128 (83.3 + 500 µg/ml) exhibited similar capability (Figures 1B, C) as the positive drug Selegiline. Stachyose is a soluble carbohydrate that works as a probiotic that reduces the negative impacts on antibiotic-destroyed microbiota (29) and improves long-term potentiation impairment caused by stress through the gut-brain axis (30). PS128 improved stress, anxiety and insomnia in humans (31).…”

Section: Discussionmentioning

confidence: 99%

Nutrient combinations exhibit universal antianxiety, antioxidant, neuro-protecting, and memory-improving activities

Zhao

Jia²,

Feng³

et al. 2023

Front. Nutr.

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Anxiety disorders are the most common mental disorders and, without proper treatment, may lead to severe conditions: e.g., somatic disorders or permanent damage to central nervous system. Although there are drugs in clinical trials, this study focuses on exploring the efficacy of nutrients in treating these diseases. We built different zebrafish models and screened several nutrient combinations for their antianxiety, antioxidant, neuro-protecting, and memory-improving activities. Our results showed that the combinations of nutrients (e.g., Walnut Peptides + Theanine at 14.2 + 33.3 μg/ml) have similar or better activities than the positive control drugs. In addition, we discovered that the effects of the nutrients in the above four aspects were universal and highly related. This study is noteworthy as it suggested that nutrients could be healthier and greener drug alternatives and provide similar or better universal treatments for anxiety and related conditions.

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Section: Discussionmentioning

confidence: 99%

Nutrient combinations exhibit universal antianxiety, antioxidant, neuro-protecting, and memory-improving activities

Zhao

Jia²,

Feng³

et al. 2023

Front. Nutr.

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“…Feeding rats a diet rich in raffinose significantly enhances the abundance of Lactobacillus spp., reduces C. perfringens, and markedly elevates the levels of acetic and propionic acids in the caecum [4]. The oral administration of stachyose demonstrated the potential to mitigate dysbiosis of the intestinal flora and inflammatory response induced by dextran sulfate sodium (DSS) [5]. The verbascose derived from mung bean is believed to possess immunomodulatory properties and exhibit potential for ameliorating inflammatory responses in mice [6].…”

Section: Introductionmentioning

confidence: 99%

The Pea Oligosaccharides Could Stimulate the In Vitro Proliferation of Beneficial Bacteria and Enhance Anti-Inflammatory Effects via the NF-κB Pathway

Cheng,

Zhao,

Qiao

et al. 2024

Foods

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The oligosaccharides extracted from the seeds of peas, specifically consisting of raffinose, stachyose, and verbascose, fall under the category of raffinose family oligosaccharides (RFOs). The effect of RFOs on intestinal microflora and the anti-inflammatory mechanism were investigated by in vitro fermentation and cell experiments. Firstly, mouse feces were fermented in vitro and different doses of RFOs (0~2%) were added to determine the changes in the representative bacterial community, PH, and short-chain fatty acids in the fermentation solution during the fermentation period. The probiotic index was used to evaluate the probiotic proliferation effect of RFOs and the optimal group was selected for 16S rRNA assay with blank group. Then, the effects of RFOs on the inflammatory response of macrophage RAW264.7 induced by LPS were studied. The activity of cells, the levels of NO, ROS, inflammatory factors, and the expression of NF-κB, p65, and iNOS proteins in related pathways were measured. The results demonstrated that RFOs exerted a stimulatory effect on the proliferation of beneficial bacteria while concurrently inhibiting the growth of harmful bacteria. Moreover, RFOs significantly enhanced the diversity of intestinal flora and reduced the ratio of Firmicutes-to-Bacteroides (F/B). Importantly, it was observed that RFOs effectively suppressed NO and ROS levels, as well as inflammatory cytokine release and expression of NF-κB, p65, and iNOS proteins. These findings highlight the potential of RFOs in promoting intestinal health and ameliorating intestinal inflammation.

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“…Previous studies have found that C. oleifera honey contains oligosaccharides (manninotriose, raffinose, and stachyose), and it can lead to the death of honeybee larvae and adult worker bees [ 12 , 13 ]. Raffinose and stachyose are classified as raffinose family oligosaccharides (RFOs), one type of prebiotic that has biological functions such as regulating gut flora, preventing inflammatory bowel disease, protecting the liver, and lowering blood sugar and blood lipids, etc., [ 14 , 15 , 16 , 17 ]. This implies the promising use of C. oleifera honey for the development of potential health promoters and dietary supplements, which is a prioritized direction for subsequent research in our laboratory.…”

Section: Introductionmentioning

confidence: 99%

Kaempferitrin: A Flavonoid Marker to Distinguish Camellia oleifera Honey

Huang

Zheng

et al. 2023

Nutrients

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C. oleifera is an economically important oilseed crop and medical plant. However, as a characteristic honey resource, the standard protocol used to identify the composition of C. oleifera honey has not been established yet. Previously, distinctive flavonoid has been shown as an effective marker to trace the botanical origin of honey. In this study, we examined the flavonoid types in C. oleifera honey and nine other monofloral honeys by using liquid chromatography tandem-mass spectrometry (LC-MS/MS) and compared the differences and identified eight distinct flavonoids in C. oleifera honey. Then, comparing the 8 flavonoids with the 14 flavonoids common to C. oleifera honey and nectar, two distinct flavonoids were identified in C. oleifera honey and nectar. Finally, we identified kaempferitrin as the distinct flavonoid marker in C. oleifera honey using the degree of influence of the partial least-squares discriminant analysis (PLS-DA) model on C. oleifera honey and ployfloral honey.

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Effects of stachyose on the intestinal microbiota and barrier in antibiotic-treated mice

Cited by 7 publications

References 23 publications

Nutrient combinations exhibit universal antianxiety, antioxidant, neuro-protecting, and memory-improving activities

Nutrient combinations exhibit universal antianxiety, antioxidant, neuro-protecting, and memory-improving activities

The Pea Oligosaccharides Could Stimulate the In Vitro Proliferation of Beneficial Bacteria and Enhance Anti-Inflammatory Effects via the NF-κB Pathway

Kaempferitrin: A Flavonoid Marker to Distinguish Camellia oleifera Honey

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