Effects of starvation stress on jade perch scortum barcoo based on proteomics analysis

Abstract

Proteins related to antioxidant defence systems and energy maintenance play a vital role in resisting starvation in fish. However, studies on the underlying molecular mechanism of starvation stress in the jade perch Scortum barcoo are very limited. Thus, the proteomic changes in S. barcoo during fasting were investigated by stable isotope labelling and high‐performance liquid chromatography‐tandem mass spectrometry (iTRAQ‐LC‐ MS/MS) in the present study. Therefore, a total of 3324 proteins were identified, including 134 that were significantly altered (p < 0.05, fold change >1.20 or <0.83) in the experimental groups compared with the control group. Among all the differentially expressed proteins, only protein‐disulphide reductase (TXNDC12) was upregulated. One‐third of the differentially expressed proteins were enriched in the amino acid metabolism pathway, tricarboxylic acid (TCA) cycle pathway, fatty acid metabolism pathway and oxidative phosphorylation pathway. Proteins differentially expressed in the experimental group, including AASS, OGDH, ACAD8, FAH, BHMT, AOC3, AOC2 and HIBADH, are components of pathways that control the metabolism of amino acids. The results suggested the involvement of antioxidant defence in the response to an oxidative imbalance, and GSH, SOD and AKP were regulated under conditions of starvation stress. In conclusion, the data indicated that starvation‐induced changes in protein levels that might affect metabolism and the antioxidant system.

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

“…During transportation, the pathways related to lipid metabolism utilization in crab muscle might upregulate to provide energy to resist starvation. Studies have shown that the compensation and content of fatty acid changed in jade perch (Scortum barcoo) during starvation, potentially impacting its flavor . The downregulation of lipid metabolism showed that the function of the cell membrane in crab muscle was impaired.…”

“…Studies have shown that the compensation and content of fatty acid changed in jade perch (Scortum barcoo) during starvation, potentially impacting its flavor. 69 The downregulation of lipid metabolism showed that the function of the cell membrane in crab muscle was impaired. This phenomenon could be attributed to the downregulation of energy metabolism and the nutritional function, potentially altering the taste of crab after transportation, thus leading to a decline in the sensory scores (Figure 1A) of crab muscle.…”

Morphological change and differential proteomics analysis of gill in Mytilus coruscus under starvation

“…Alizadeh et al (2021) found that the purine metabolism capacity of Russian sturgeon was reduced after prolonged starvation. Moreover, Xie et al (2021) found that downregulated proteins in the purine and pyrimidine metabolism pathways were enriched in Australian jewel perch after starvation. The reduction of all three metabolic capacities is inseparable from reducing energy expenditure.…”

Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

“…Among the five common pathways, the TCA cycle is central to the final metabolic pathway of the three major energy sources [ 69 ]. These substrates generated from energy stores may participate in the TCA cycle, an aerobic process for producing ATP by oxidating acetate derived from energy reserves [ 64 , 70 ], thereby satisfying the energy demands for fish.…”