Effects of static magnetic fields on bone regeneration of implants in the rabbit: micro‐<scp>CT</scp>, histologic, microarray, and real‐time <scp>PCR</scp> analyses

Kim, Eun-Cheol; Liu, Richard; Lee, Sung‐Hee; Hong, Ji Youn; Ko, Eunjin; Ahn, Su−Jin

doi:10.1111/clr.12812

Cited by 28 publications

(21 citation statements)

References 37 publications

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“…These magnetic materials normally generate a static magnetic field (SMF), and the flux leakages spread to oral adjacent tissues (Riley et al, ; Yagci & Kesim, ). Multiple studies have investigated the biological effect of magnetic fields on alveolar bone, gingiva, and dental pulp (Bondemark, Kurol, & Larsson, ; Kim, et al, ). The Kim et al () study demonstrated that the magnetic field and osseointegration have the positive correlation.…”

Section: Introductionmentioning

confidence: 99%

“…Multiple studies have investigated the biological effect of magnetic fields on alveolar bone, gingiva, and dental pulp (Bondemark, Kurol, & Larsson, ; Kim, et al, ). The Kim et al () study demonstrated that the magnetic field and osseointegration have the positive correlation. Bondemark et al () indicated that there are no changes in human dental pulp or gingival tissue adjacent to the magnet implants.…”

Section: Introductionmentioning

confidence: 99%

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Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Zheng

Zhang

Chen

et al. 2018

J Tissue Eng Regen Med

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The dental pulp stem cells (DPSCs) are a population of mesenchymal stem cells, which have multilineage potential and high proliferation. DPSCs are regarded as a promising tool for tissue regeneration of dentine, dental pulp, bone, cartilage, and muscle. Recently, magnetic materials have become commonly applied in dental clinics. Static magnetic field has been reported to regulate the proliferation, migration, or differentiation of stem cells. However, whether static magnetic fields affect DPSCs is still unknown. In our study, we investigated the effect of static magnetic field on the proliferation, migration, and differentiation of DPSCs. The results indicated that static magnetic field rearranged the cytoskeleton of DPSCs. A static magnetic field of 1 mT increased DPSC proliferation, as well as the gene expression of several growth factors such as FGF-2, TGF-β, and VEGF. Moreover, the static magnetic field promoted the migration of DPSCs by regulating MMP-1 and MMP-2 gene expression. Static magnetic field of 1 mT also induced osteo/odontogenesis and mineralization in DPSCs. Otherwise, the static magnetic field recruited YAP/TAZ to the nucleus, inhibited the phosphorylation of YAP/TAZ, and upregulated the two YAP/TAZ-regulated genes, CTGF and ANKRD1. Cytoskeleton inhibitor, cytochalasin D, obviously inhibited the nuclear localization of YAP/TAZ. When YAP/TAZ were knocked-down, the static magnetic field-induced mineralization of DPSCs was diminished. Our findings provide an insight into the effect of static magnetic field on DPSCs and provide the foundation for the future tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Zheng

Zhang

Chen

et al. 2018

J Tissue Eng Regen Med

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“…COL12A1 is a protein that is a member of the fibril-associated collagens with interrupted triple helix (FACIT) collagen family, which is related to the ECM –receptor interaction, PPAR-Peroxisome proliferator-activated receptor and the MAPK-Mitogen-activated protein kinase signaling pathway ( Kim et al, 2016 ; Punetha et al, 2017 ; Zhang et al, 2018 ; Xiang et al, 2019 ). COL12A1 is associated with joint anomalies, gastric cancer, myopathies, ECM defects, and chondromyxoid fibroma.…”

Section: Discussionmentioning

confidence: 99%

Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis

Wang

et al. 2020

Front. Pharmacol.

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The aim of this study was to investigate the precise clinical use of Sinitang decoction (SNT) in ulcerative colitis (UC). Network pharmacology-based analysis of the drug components–targets–diseases–pathways was used to predict the possible clinical applications of SNT. Next, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was used to establish a rat model of UC, and the efficacy of SNT against UC was tested, followed by a proteomic analysis of the specific signatures regulated by SNT against UC. SNT was predicted to be effective in inflammatory bowel disease, UC, and several other diseases. In the rats with UC, SNT decreased the disease activity index and colon mucosal damage index compared to the untreated UC model rats. Additionally, SNT reversed the upregulated levels of serum tumor necrosis factor (TNF)-α, prostaglandin E 2 (PGE 2 ), interleukin (IL)-6, and nitric oxide (NO) in UC model rats. The proteomic analysis identified 78 proteins that were differentially regulated by SNT in the rats with UC, which were associated with the Gene Ontology terms sulfur compound binding, calcium ion binding, and Toll-like receptor (TLR)-4 binding. Among these differentially regulated proteins, C-reactive protein (CRP) and collagen alpha-1(XII) chain (COL12A1) were found to be signature proteins associated with the efficacy of SNT against UC. This study represents the first precise investigation of the efficacy and mechanisms of SNT against UC, and shows that SNT is a promising candidate for personalized management of UC.

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“…While it is beyond the remit of this review to discuss in details studies on implant osseointegration, it is worth mentioning that animal and human studies applying whole‐genome analyses on peri‐implant tissues and cells have also contributed to elucidate the sequence of gene activation during bone formation and they have confirmed that, while immuno‐inflammatory response‐ and ECM‐associated genes are the ones most highly expressed during the early days, the later stages of organization of the granulation tissue and formation of woven bone involve the upregulation of angiogenesis, osteogenesis and neurogenesis genes. [ 84–91 ] We previously published transcriptomic data from trephine bone core specimens retrieved together with cylindrical titanium implants from the retromolar areas of healthy volunteers at different time points (4, 7, and 14 days). Our results indicated that immune‐inflammatory pathways, such as the I‐kB kinase/nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐kB) cascade, were upregulated during the first healing days, whilst TGF‐β/BMP, Notch and Wnt signaling pathways were significantly expressed at the later healing time.…”

Section: Omics Approaches To Characterize the Bone Regeneration Procementioning

confidence: 99%

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

Calciolari

Donos

2020

Proteomics Clinical Apps

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Bone regeneration is a complex biological process, where the molecular mechanisms are only partially understood. In an ageing population, where the prevalence of chronic diseases with an impact on bone metabolism is increasing, it becomes crucial to identify new strategies that would improve regenerative outcomes also in medically compromised patients. In this context, omics are demonstrating a great potential, as they offer new insights on the molecular mechanisms regulating physiologic/pathologic bone healing and, at the same time, allow the identification of new diagnostic and therapeutic targets. This review provides an overview on the current evidence on the use of transcriptomic and proteomic approaches in bone regeneration research, particularly in relation to type 1 diabetes and osteoporosis, and discusses future scenarios and potential benefits and limitations on the integration of multi-omics. It is suggested that future research will leverage the synergy of omics with statistical modeling and bioinformatics to prompt the understanding of the biology underpinning bone formation in health and medically compromised conditions. With an eye toward personalized medicine, new strategies combining the mining of large datasets and bioinformatic data with a detailed characterization of relevant phenotypes will need to be pursued to further the understanding of disease mechanisms.

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Effects of static magnetic fields on bone regeneration of implants in the rabbit: micro‐CT, histologic, microarray, and real‐time PCR analyses

Abstract: μCT, histology, microarrays, and real-time PCR indicate that SMFs could be an effective approach to improving bone regeneration around dental implants.

Cited by 28 publications

References 37 publications

Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

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