Effects of Stem Cell Therapy on Left Ventricular Remodeling After Acute Myocardial Infarction: A Meta‐Analysis

Sun, Lan; Zhang, Tiantai; Lan, Xi; Du, Guanhua

doi:10.1002/clc.20772

Cited by 25 publications

(8 citation statements)

References 39 publications

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“…33–35 Despite the poor retention rates, small improvements in cardiac function and reduction in ventricular scarring have been reported, providing evidence of potential therapeutic utility. 36,37 Such in vivo studies can be challenging and expensive, making it difficult to iteratively test and develop new strategies for improved efficacy. Another common challenge of cell-based therapies is the often-limited source and quantity of the therapeutic cells, which can preclude the use of many conventional adhesion assays, whether in vivo or in vitro.…”

Section: Resultsmentioning

confidence: 99%

High-content adhesion assay to address limited cell samples

et al. 2013

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Cell adhesion is a broad topic in cell biology that involves physical interactions between cells and other cells or the surrounding extracellular matrix, and is implicated in major research areas including cancer, development, tissue engineering, and regenerative medicine. While current methods have contributed significantly to our understanding of cell adhesion, these methods are unsuitable for tackling many biological questions requiring intermediate numbers of cells (102–105), including small animal biopsies, clinical samples, and rare cell isolates. To overcome this fundamental limitation, we developed a new assay to quantify the adhesion of ~102–103 cells at a time on engineered substrates, and examined the adhesion strength and population heterogeneity via distribution-based modeling. We validated the platform by testing adhesion strength of cancer cells from three different cancer types (breast, prostate, and multiple myeloma) on both IL-1β activated and non-activated endothelial monolayers, and observed significantly increased adhesion for each cancer cell type upon endothelial activation, while identifying and quantifying distinct subpopulations of cell-substrate interactions. We then applied the assay to characterize adhesion of primary bone marrow stromal cells to different cardiac fibroblast-derived matrix substrates to demonstrate the ability to study limited cell populations in the context of cardiac cell-based therapies. Overall, these results demonstrate the sensitivity and robustness of the assay as well as its ability to enable extraction of high content, functional data from limited and potentially rare primary samples. We anticipate this method will enable a new class of biological studies with potential impact in basic and translational research.

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Section: Resultsmentioning

confidence: 99%

High-content adhesion assay to address limited cell samples

et al. 2013

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“…Moreover, intracoronary cell therapy has been reported to be accompanied with a decrease in incidence of death, recurrent of acute myocardial infarction, and readmission for heart failure 294,297 . At the present time, however, clinical reports regarding the effect of intracoronary stem/progenitor cell treatment on the infarct volume and remodeling remain inconsistent 294,295,297,299 . A phase 3, randomized, double-blinded, active-controlled, unblinded standard of care study (the RENEW study) is underway to assess the efficacy and safety of intramyocardial administration of autologous CD34 + cells in patients with refractory angina 300 .…”

Section: Application Of Vascular Precursor Cells In the Treatment Of mentioning

confidence: 98%

Vascular precursor cells in tissue injury repair

Shi

Zhang

Yin

et al. 2017

Translational Research

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Vascular precursor cells include stem and progenitor cells giving rise to all mature cell types in the wall of blood vessels. Upon tissue injury, local hypoxia and inflammation result in generation of vasculogenic mediators which orchestrate migration of vascular precursor cells from their niche environment to the site of tissue injury. The intricate crosstalk among signaling pathways coordinates vascular precursor cell proliferation and differentiation during neovascularization. Establishment of normal blood perfusion plays an essential role in effective repair of the injured tissue. In recent years, studies on molecular mechanisms underlying the regulation of vascular precursor cell function have achieved substantial progress, which promotes exploration of vascular precursor cell-based approaches to treat chronic wounds and ischemic diseases in vital organ systems. Verification of safety and establishment of specific guidelines for the clinical application of vascular precursor cell-based therapy remain major challenges in the field.

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“…To disentangle such a complex subject, a series of metaanalyses (MAs) were conducted during the past 8 years: 12 were focused on patients with acute MI (AMI), [15][16][17][18][19][20][21][22][23][24][25][26] 7 evaluated cell therapy in the context of CIHD, [27][28][29][30][31][32][33] and 4 took into account both conditions. [34][35][36][37] Although, overall, 81% and 92% of MAs on AMI and CIHD, respectively, yielded positive outcomes (Figure 1), it has been recently suggested that published MAs on BMC therapy for IHD far outnumber wellconducted RCTs.…”

Section: Article See P 558mentioning

confidence: 99%