Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis

Huang, Guo-Yong; Ying, Guang‐Guo; Liang, Yan‐Qiu; Liu, You-Sheng; Liu, Shuangshuang

doi:10.1016/j.cbpc.2013.05.001

Cited by 17 publications

(21 citation statements)

References 41 publications

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“…However, our previous study reported the concentration ranges of 45.7 to 1743 ng/L for OCPs and 0.051 to 4.32 ng/L for PCBs in the investigated Dongjiang River basin . In addition, the effects of metals or steroid hormones on EROD activity and CYP1A mRNA expression cannot be ignored, where mosquitofish are exposed to complex mixtures including metals and steroid hormones in the two rivers , Huang et al, 2013Huang et al, 2012;Lavado et al, 2006;Ying et al, 2012). It is noted in the present study that the correlations between EROD activity and CYP1A mRNA expression appear to be sex specific, which might be due to sex-related differences in P450 activity.…”

Section: Discussionmentioning

confidence: 99%

“…Real-time quantitative PCR amplification was performed with the Applied Biosystems ViiA™ 7 Dx (ABI) using the THUNDERBIRD SYBR® qPCR Mix (Toyobo) (Toyobo). Primer sequences for G. affinis MT, CYP1A and β-Actin were obtained from Huang et al (2013). Primer sequences for G. affinis ribosomal protein L8 (RPL8) gene were obtained from Huang et al (2016).…”

Section: Mt and Cyp1a Mrna Expression Analysismentioning

confidence: 99%

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Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Huang

Liu

Liang

et al. 2016

Science of The Total Environment

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Section: Discussionmentioning

confidence: 99%

Section: Mt and Cyp1a Mrna Expression Analysismentioning

confidence: 99%

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Huang

Liu

Liang

et al. 2016

Science of The Total Environment

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“…Isolation of total RNA was carried out using Trizol reagent (Invitrogen) as described by Huang et al [18]. Total RNA concentration was determined at 260 nm using a SmartSpec TM Plus Spectrophotometer (Bio-Rad), and the purity and quality were analyzed by measuring 260/280 nm ratios.…”

Section: Isolation Of Rna and Reverse Transcriptionmentioning

confidence: 99%

“…According to our previous methods [18], quantitative realtime PCR was performed on the Applied Biosystems ViiA TM 7 Dx (ABI) using the THUNDERBIRD SYBR 1 qPCR Mix (Toyobo). The Primer Premier 5.0 program was used to design the primer sequences for the target and housekeeping genes according to the following criteria ( Table 1).…”

Section: Quantitative Real-time Polymerase Chain Reactionmentioning

confidence: 99%

“…More importantly, Zucchi et al [17] determined the effects of 4 different progestins on transcriptional changes of steroid hormone receptors in zebrafish and found that progestins may disrupt sex differentiation and consequently impair reproduction by modulating some gene transcripts. Progestins are widely known to bind to progesterone receptors; and they also have some interactions with other steroid hormone receptors-such as androgen, estrogen, glucocorticoid, and mineralocorticoid receptors-and exhibit different mechanisms of action [17][18][19]. In addition, levonorgestrel has been reported to interfere with the messenger (m)RNA expression of steroidogenic genes in fathead minnows [20].…”

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confidence: 99%

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A time‐course transcriptional kinetics of the hypothalamic–pituitary–gonadal and hypothalamic–pituitary–adrenal axes in zebrafish eleutheroembryos after exposure to norgestrel

Liang

Huang

Ying

et al. 2014

Enviro Toxic and Chemistry

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Abstract:The objective of the present study was to investigate the effects of norgestrel on the hypothalamic-pituitary-gonadal (HPG) and hypothalamic-pituitary-adrenal (HPA) axes in zebrafish eleutheroembryos. Zebrafish embryos were exposed to different concentrations, and 100 ng L À1) for 144 h post fertilization (hpf), and the transcriptional profiles of the HPG and HPA axes were examined every day. Norgestrel modulated the expression of Pgr and Vtg1 messenger (m)RNAs mainly at 96 hpf for all treatment groups. In addition, norgestrel strongly altered the expression of Cyp11a1 mRNA above 5 ng L À1 (significant upregulation from 48 hpf to 120 hpf and significant downregulation for 144 hpf). Norgestrel treatment could significantly induce expression of Cyp19a1a, Cyp11b, Gnrh2, Gnrh3, and Lhb mRNAs but inhibit transcripts of Hsd11b2 and Crh genes above 5 ng L À1 at different time points. The transcriptional expression levels of Esr1, Ar, Star, Hsd17b3, Fshb, and Pomc were also mediated by 5 ng L À1 norgestrel or higher during different exposure periods. Taken together, the overall results imply that the transcriptional changes in zebrafish eleutheroembryos may pose a potential effect on embryonic development, in particular in the brain and gonadogenesis. Environ Toxicol Chem 2015;34:112-119. # 2014 SETAC

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Norethindrone alters growth, sex differentiation and gene expression in marine medaka (Oryzias melastigma)

Dong

Chen

et al. 2022

Environmental Toxicology

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Norethindrone (NET) is a widely used synthetic progestin, which appears in water environments and threatens aquatic organisms. In this study, marine medaka (Oryzias melastigma) larvae were exposed to 7.6 and 80.1 ng/L NET for 190 days. The effects of NET on growth, sex differentiation, gonad histology and transcriptional expression profiles of hypothalamic-pituitary-gonadal (HPG) axis-related genes were determined. The results showed that exposure to 80.1 ng/L NET caused an all-male marine medaka population and significantly decreased the growth of males. Exposure to 7.6 ng/L NET increased the ratio of males/females in the marine medaka population, decreased the growth of males and delayed the ovary maturation in females.However, the sperm maturation was accelerated by 7.6 or 80.1 ng/L NET. In females, the transcription levels of cytochrome P450 aromatase (cyp19a1a) and progesterone receptor (pgr) in ovaries, glucocorticoid receptor (gr) and vitellogenin (vtg) in livers were suppressed after exposure to 7.6 ng/L NET, which may cause delayed ovary maturation. In males, NET significantly decreased the transcription levels of follicle stimulating hormone β (fshβ) and Luteinizing hormone β (lhβ)in the brain, Estrogen receptor β (erβ)，gr and pgr in the liver, and vitellogenin receptor (vtgr) in the testes, while NET of 80.1 ng/L led to a significant up-regulation of steroidogenic acute regulatory protein (star) in the testes of males. These results showed that NET could influence growth, sex differentiation and gonadal maturation and significantly alter the transcriptional expression levels of HPG axis-related genes.

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Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis

Cited by 17 publications

References 41 publications

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

A time‐course transcriptional kinetics of the hypothalamic–pituitary–gonadal and hypothalamic–pituitary–adrenal axes in zebrafish eleutheroembryos after exposure to norgestrel

Norethindrone alters growth, sex differentiation and gene expression in marine medaka (Oryzias melastigma)

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