Effects of Steroid‐Induced <i>Pneumocystis carinii</i> on Alveolar Macrophages in the Rat

Shear, Hannah L.; El‐Sadr, Wafaa; RUBINSTIEN, BRUCE E.; Ferreira, M. Teresa S. O. B.

doi:10.1111/j.1550-7408.1989.tb05828.x

Cited by 2 publications

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“…Trophozoite, cyst, and other intermediate stages of P. carinii exist (28), and P. carinii isolates from different animals may contain mixtures of P. carinii forms that differ in their ability to elicit an oxidative burst. Another possibility is that the amounts of complement (21) and immunoglobulins (4) found on the surface of P. carinii isolated from the lungs may vary among different P. carinii isolates.…”

Section: Discussionmentioning

confidence: 99%

Pneumocystis carinii induces an oxidative burst in alveolar macrophages

et al. 1992

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There is evidence that alveolar macrophages (AM) play a role in the clearing of Pneumocystis carinii from the lungs. To investigate the mechanisms involved in this process, we studied in vitro the induction of an oxidative burst by P. carinii in a cell line of macrophages (NR8383) and AM from normal rats. P. carinui was added to macrophage monolayers (106 cells), and the H202 produced after 4 h of incubation was measured. Both NR8383 macrophages and normal rat AM produced H202 in response to P. carinii cysts and trophozoites isolated from dexamethasone-treated rats, although the amount of H202 induced in AM from normal rats was larger. NR8383 macrophages bound and phagocytized both P. carinii cysts and trophozoites and produced increasing amounts of H202 as a dose-related response to cysts and trophozoites. Opsonization ofP. carinii with normal rat serum increased H202 production by both types of macrophages; this enhancement was decreased, but not abolished, when the serum was first depleted of complement by heat treatment. These findings demonstrate that NR8383 macrophages and normal rat AM produce an oxidative burst in response to P. carinii and that this response is enhanced by complement.

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Section: Discussionmentioning

confidence: 99%