Effects of stocking density of Labeo rohita on survival, growth and production in cages

Chattopadhyay, D. N.; Mohapatra, B. C.; Adhikari, Sanjeev; Pani, K. C.; Jena, J. K.; Ambekar, Jalindar D.

doi:10.1007/s10499-012-9528-2

Cited by 35 publications

(27 citation statements)

References 13 publications

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“…Raw water sample (n=6) are taken for analysis of glyphosate concentration. For field experiment a special type of cage was prepared and installed separately at pond of CRSMF as per Chattopadhyay et al [12] with some modifications. All the cages were square in shape having an area of 2.5 m x 1.22 m and height of the cage was 1.83 m (submerged height was 0.83 m).…”

Section: Methodsmentioning

confidence: 99%

Histopathological and Ultrastructural Alterations in Anabas testudineus Exposed to Glyphosate-Based Herbicide, Excel Mera 71 under Field and Laboratory Conditions

Samanta¹,

Pal²

2016

J Aquac Res Development

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Section: Methodsmentioning

confidence: 99%

Histopathological and Ultrastructural Alterations in Anabas testudineus Exposed to Glyphosate-Based Herbicide, Excel Mera 71 under Field and Laboratory Conditions

Samanta¹,

Pal²

2016

J Aquac Res Development

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“…Recommended dose (8 g/acre) for rice cultivation was dissolved in water and was sprayed on the first day on the surface of almix-treated plots [12][13][14][15]. Special type of cage was prepared for field experiments and was installed separately at pond of university CRSMF based on Chattopadhyay et al [16] with some modifications. Cages were rectangular in shape (2.5 m × 1.22 m × 1.83 m) and the submerged height in water was 0.83 m. Cages were prepared by using strong bamboo.…”

Section: Field Experimental Designmentioning

confidence: 99%

Cytopathological Effects of Almix Herbicide on Gill, Liver and Kidney of Oreochromis niloticus under Field and Laboratory Conditions

Samanta¹,

Pal²,

Mukherjee³

et al. 2016

Toxicol Open Access

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Objective: Present study was aimed to study the cytopathological effects of almix herbicide in Indian freshwater teleost, Oreochromis niloticus (Linn.) both under field and laboratory exposure. Methods:O. niloticus (Linn.) was exposed to almix herbicide at sublethal concentrations of 8 g/acre and 66.67 mg/L under field and laboratory conditions respectively for 30 days. Field experiment was performed in cage, submerged in the field pond. Cytopathological study both through light and electron microscopic (scanning electron microscopy and transmission electron microscopy) observations were based on gill, liver and kidney.Results: Histopathological observations revealed hypertrophy and proliferation in gill epithelium, curling and fusion of secondary gill lamellae (SGL), distortion in chloride and pillar cells under laboratory experiment. Scanning electron microscopy displayed severe loss of normal array of concentric microridges, swelling of microridges, damage in epithelial cells and appearance of vacuoles on the stratified epithelium, while loss of regular pattern of microridges were observed under field condition. Transmission electron microscopic study of gill showed severe damages which included degenerative changes in mitochondria, cellular vacuolation, damage in tubule vascular system, presence of lipid droplets, elongated nucleus, but in case of field experiment dilated mitochondria and cytoplasmic vacuolation were more prominent. Degenerative hepatopancreas, necrosis in hepatocytes, severe cytoplasmic vacuolation and disarrangement of hepatic cords in liver of O. niloticus were prominent seen under light microscopy in the laboratory condition, but in field condition elongated hepatocytes with increased nuclei and vacuolation in cytoplasm of hepatocytes were prominent, while TEM study showed degeneration in mitochondria, dilation in rough endoplasmic reticulum, damage in nucleus and appearance of cytoplasmic vacuolation in hepatocytes under laboratory condition. In kidney, degenerative changes in PCT and DCT, shrinkage of glomerulus, vacuolation in the haematopoietic tissues and excess fat deposition were notable changes. In TEM study necrosis in nucleus, severe vacuolation, appearance of endoplasmic reticulum as whorl pattern, degeneration in mitochondria in kidney were serious after almix exposure. Conclusion:Present study claimed the more profound responses under laboratory condition compared to field and different significant marked changes in these two conditions were prominent. Therefore, these responses in gill, liver and kidney of O. niloticus could be considered as potential biomarkers of exposure of agrochemicals.

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“…The duration of the experiment was 30 days. Special type of cage was prepared based on the method described by Chattopadhyay with slight modifications and installed at the ponds of CRSMF premises [21]. Cages were square in shape with the dimension of 2.5 m × 1.22 m × 1.83 m (submerged height was 0.83 m) and were structured by light strong bamboo.…”

Section: Field Experimental Designmentioning

confidence: 99%

Zebrafish in the Wild: Microhabitat Use by Zebrafish Danio rerio (Hamilton, 1822) from Karala River of Jalpaiguri District, Northern Bengal, India

Raja¹,

Raja²,

Perumal³

2016

Fish Aquac J

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The aim of the present study was to investigate the histopathological alterations in the stomach and intestine of Indian freshwater teleost, Anabas testudineus (Bloch, 1792) after Almix® exposure both under laboratory and field conditions. The field (dose 8 g/acre) and laboratory (dose 66.67 mg/l) experiments was carried out for 30 days. Special type of cage was prepared and installed in the pond for the field experiment. Pathological alterations in the concerned fish organs namely stomach and intestine were assessed through light microscopy, scanning and transmission electron microscopy. Lesions observed under light microscopy also endorsed the findings of ultrastructural observations both under laboratory and field conditions. Cytopathological alterations observed under light and electron microscopy revealed that the degree of responses were different in different fish tissues as well as under conditions, here in particular effects in stomach were more prominent in laboratory condition. The overall responses registered in the fish tissues under laboratory condition were more pronounced than field condition. Therefore, these symptoms and/or alterations in the present study due to almix intoxication could be considered as biomarkers in toxicity study in aquatic ecosystem.

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Effects of stocking density of Labeo rohita on survival, growth and production in cages

Cited by 35 publications

References 13 publications

Histopathological and Ultrastructural Alterations in Anabas testudineus Exposed to Glyphosate-Based Herbicide, Excel Mera 71 under Field and Laboratory Conditions

Histopathological and Ultrastructural Alterations in Anabas testudineus Exposed to Glyphosate-Based Herbicide, Excel Mera 71 under Field and Laboratory Conditions

Cytopathological Effects of Almix Herbicide on Gill, Liver and Kidney of Oreochromis niloticus under Field and Laboratory Conditions

Zebrafish in the Wild: Microhabitat Use by Zebrafish Danio rerio (Hamilton, 1822) from Karala River of Jalpaiguri District, Northern Bengal, India

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