Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa

Dziekońska, Anna; Zasiadczyk, Łukasz; Lecewicz, Marek; Strzeżek, Rafał; Koziorowska-Gilun, Magdalena; Fraser, Leyland; Mogielnicka-Brzozowska, Marzena; Kordan, Władysław

doi:10.1515/pjvs-2015-0095

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…The addition of Zn 2+ to the preservation diluent can effectively compensate for the removed SP. Semen contains higher concentrations of antioxidants and Zn than any other biological fluid (Dziekońska et al., 2018 ). One of the most active antioxidant enzyme in semen is copper‐zinc superoxide dismutase (CuZnSOD).…”

Section: Discussionmentioning

confidence: 99%

Effects of zinc chloride on boar sperm quality during liquid storage at 17°C

2023

Veterinary Medicine & Sci

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Objectives:The aim of this study was to evaluate the effects of zinc chloride (ZnCl 2 ) at 20, 50, 100, and 200 μg/mL on the quality of seminal plasma-free boar sperm stored at 17 • C for 7 days and to explore the underlying mechanisms.Methods: Boar sperm were collected and incubated in non-capacitation/capacitation medium to analyze sperm quality. Results:In the non-capacitated state, the addition of ZnCl 2 at 20 and 50 μg/mL improved the survival rate and plasma membrane integrity of boar sperm (p < 0.05).Compared to the control group, the addition of ZnCl 2 significantly increased total antioxidative capacity and CuZn superoxide dismutase activity, while reducing the malondialdehyde content (p < 0.05). ZnCl 2 at 100 and 200 μg/mL significantly decreased sperm motility, protein kinase A (PKA) substrate phosphorylation, and tyrosine phosphorylation. These proteins were mainly located on the mid-pieces of the flagellum.The addition of ZnCl 2 at 20 and 50 μg/mL conveyed a protective effect to boar sperm stored at 17 • C. Furthermore, ZnCl 2 at 100 and 200 μg/mL inhibited sperm motility via tyrosine phosphorylation, thus preventing the 'capacitation-like' state. In the capacitated state, there was no change in PKA substrate phosphorylation and tyrosine phosphorylation of the mid-pieces of the flagellum compared to the control groups, indicating that the addition of Zn 2+ did not negatively affect capacitation of preserved sperm. Conclusions:ZnCl 2 showed protective capacity to the preservation extender used for boar sperm during the process of 17 • C storage, and the optimal concentration of ZnCl 2 for the preservation extender was 100 μg/mL.

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Section: Discussionmentioning

confidence: 99%

Effects of zinc chloride on boar sperm quality during liquid storage at 17°C

2023

Veterinary Medicine & Sci

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“…However, the susceptibility for cold shock is dependent on many different factors, and recently there have been reports of successfully sustained sperm quality at low temperature through optimization of surrounding conditions 15 , 35 , 36 . Amongst others, boar individual eligibility 37 , 38 , the cooling rate 15 , 39 , the used extender 40 , motionless storage 41 and transport 42 play important roles in the maintenance of sperm quality.…”

Section: Discussionmentioning

confidence: 99%

Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load

Hensel¹,

Jakop²,

Scheinpflug

et al. 2020

Sci Rep

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Antimicrobial resistance is a steadily increasing problem and poses a serious threat to global public health. Therefore, it is highly necessary to advance the development of novel antimicrobial compounds and semen preservation strategies. The aim of this study was to evaluate a low temperature, antibiotic-free preservation procedure using Androstar Premium (ASP) extender (Minitüb) with antimicrobial lipopeptides. Firstly, seven lipopeptides in two concentrations (1 × minimum inhibitory concentration (MIC)/2 × MIC) were tested on their sperm-compatibility at 17 °C. Two lipopeptides, C16-KKK-NH 2 and C16-KKKK-NH 2 , did not negatively affect sperm quality and were further evaluated for their efficiency of bacterial growth inhibition at 5 °C. Besides an overall diminution of colony forming units, both peptides showed a reduction of bacterial subcultures (n = 103) with a decrement in Gram-positive rods from 65 (ASP w/o supplements) to 39/52 (ASP w/ C16-KKK-NH 2 /C16-KKKK-NH 2), in Gram-positive cocci from 21 to 9/10 and in Gram-negative species from 17 to 8/5 total subcultures. Furthermore, lipopeptides revealed activity towards selected bacteria of potential concern in artificial insemination like Trueperella pyogenes, Alcaligenes faecalis, Pseudomonas aeruginosa (not C16-KKK-NH 2), Pasteurella sp., Providencia stuartii, Escherichia coli (not C16-KKKK-NH 2) and Streptococcus porcinus (not C16-KKKK-NH 2). Consequently, both tested lipopeptides are promising candidates for alternative antibiotic-free preservation techniques of boar semen. Spreading antimicrobial resistance is a growing problem in human and veterinary medicine, poses a serious threat to global public health 1 and should therefore be addressed in a multifaceted approach from all areas of concern, including the industry branch of artificial insemination (AI) in pigs. It has been proven, that unhindered bacterial growth has detrimental effects on several sperm quality characteristics 2 and a transmission of potential pathogens to the inseminated sow has to be prevented in order to minimize negative effects on fertility and litter size 3. Semen collection is a non-sterile process and AI doses are conventionally stored at temperatures between 16 and 18 °C to prevent cold shock injuries to the highly sensitive boar spermatozoa 4. The combination of moderate temperatures and the use of nutrient-rich semen extenders promotes bacterial growth of psychrophilic and mesophilic species 5. As a countermeasure, antibiotics like gentamicin sulphate are routinely added to boar semen preserved for AI (Council Directive, European Union, 90/429/EEC). A steadily growing portion of contaminant bacteria isolated from AI doses are resistant to antibiotics commonly used as additives in semen extenders 6 , threatening animal welfare. This has far-reaching consequences for public health because of the natural backflow of parts of the rather large-volumed AI doses after insemination 7 ,

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