Effects of storage on brown rice (<i>Oryza sativa</i> L.) metabolites, analyzed using gas chromatography and mass spectrometry

Wang, Changyuan; Feng, Yu‐Long; Zhang, Shu; Fu, Tianxin; Sheng, Ya‐Nan; Zhang, Yiwei; Jiang, Yingjun; Yu, Miao; Zhang, Liyuan; Zhang, Dongjie

doi:10.1002/fsn3.1589

Cited by 18 publications

(7 citation statements)

References 7 publications

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“…Prolonged storage under high-temperature and high-humidity conditions results in reduced seed quality and vigor, making seeds less viable after sowing [ 36 , 37 , 38 , 39 ]. Similarly, our results demonstrate that seed aging during storage leads to decreased germination and loss of seed vigor, which is irreversible ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

ABA Inhibits Rice Seed Aging by Reducing H2O2 Accumulation in the Radicle of Seeds

Zheng,

Teng,

Zhang

et al. 2024

Plants

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The seed, a critical organ in higher plants, serves as a primary determinant of agricultural productivity, with its quality directly influencing crop yield. Improper storage conditions can diminish seed vigor, adversely affecting seed germination and seedling establishment. Therefore, understanding the seed-aging process and exploring strategies to enhance seed-aging resistance are paramount. In this study, we observed that seed aging during storage leads to a decline in seed vigor and can coincide with the accumulation of hydrogen peroxide (H2O2) in the radicle, resulting in compromised or uneven germination and asynchronous seedling emergence. We identified the abscisic acid (ABA) catabolism gene, abscisic acid 8′-hydroxylase 2 (OsABA8ox2), as significantly induced by aging treatment. Interestingly, transgenic seeds overexpressing OsABA8ox2 exhibited reduced seed vigor, while gene knockout enhanced seed vigor, suggesting its role as a negative regulator. Similarly, seeds pretreated with ABA or diphenyleneiodonium chloride (DPI, an H2O2 inhibitor) showed increased resistance to aging, with more robust early seedling establishment. Both OsABA8ox2 mutant seeds and seeds pretreated with ABA or DPI displayed lower H2O2 content during aging treatment. Overall, our findings indicate that ABA mitigates rice seed aging by reducing H2O2 accumulation in the radicle. This study offers valuable germplasm resources and presents a novel approach to enhancing seed resistance against aging.

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Section: Discussionmentioning

confidence: 99%

ABA Inhibits Rice Seed Aging by Reducing H2O2 Accumulation in the Radicle of Seeds

Zheng,

Teng,

Zhang

et al. 2024

Plants

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“…"Differential metabolites" refer to the substances found in two samples that show significant quantitative differences. The metabolites responsible for the separation were determined based on VIP values (≥1) [32]. As shown in Figure 6a, a comparison between the control and EF treatment groups identified 11 differential metabolites, with two metabolites downregulated in abundance following the EF treatment (including 15-Methylnonacosane (CAS: 65820-60-2 and CAS: 4754-26-1) and octacosane (CAS: 630-02-4)).…”

Section: Screening and Analysis Of Differentially Regulated Metabolit...mentioning

confidence: 99%

Toxicity of Ethyl Formate to Tribolium castaneum (Herbst) Exhibiting Different Levels of Phosphine Resistance and Its Influence on Metabolite Profiles

Shan,

You,

et al. 2024

Agriculture

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Ethyl formate (EF), a naturally occurring fumigant, has attracted widespread attention owing to its low toxicity in mammals. Here, Direct Immersion Solid-Phase Microextraction (DI-SPME) was employed for sample preparation in mass spectrometry-based untargeted metabolomics to evaluate the effects on Tribolium castaneum (Herbst) strains with different levels of PH3 resistance (sensitive, TC-S; moderately resistant, TC-M; strongly resistant, TC-SR) when exposed to a sub-lethal concentration (LC30) of EF. The bioassay indicated that T. castaneum strains with varying PH3 resistance levels did not confer cross-resistance to EF. A metabolomic analysis revealed that exposure to sublethal doses of EF significantly altered 23 metabolites in T. castaneum, including 2 that are unique to the species which remained unaffected by external conditions, while 11 compounds showed a strong response. A pathway topology analysis indicated that EF caused changes to several metabolic pathways, mainly involving fatty acids and their related metabolic pathways. This study showed that EF can induce highly similar metabolic responses in insects across varying levels of PH3 resistance, suggesting that the mechanisms driving the toxicity of EF and PH3 are distinct. These insights significantly extend our knowledge of the toxic mechanisms of EF and provide direct evidence for the efficacy of EF treatment for managing PH3 resistance in insects.

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“…Metabolites in rice seeds not only reflect the overall metabolic state, but also significantly impact the quality of rice [ 12 , 13 ]. However, the metabolites in rice seeds of different varieties have not been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Non-targeted GC–MS metabolomics-based differences in Indica rice seeds of different varieties

Zhou,

Jing,

Yuan

et al. 2024

BMC Plant Biol

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Rice seeds of different varieties exhibited distinct metabolic profiles in our study. We analyzed the metabolites in seeds of six rice varieties (CH, HM, NX, YX, HY, and MX) using non-targeted GC–MS. Our findings revealed that amino acids, sugars, and organic acids were predominant in all varieties, with significant differences observed in CH compared to the others. Specifically phenylalanine and glycine content differed notably in NX and YX, respectively. Additionally, 1,5-anhydroglucitol content in NX, and glutamate, aspartate, and lactulose in NX, YX, HM, HY, and MX were up-regulated. Due to the biological functions of these amino acids and sugars, these indicated that compared to CH, rice of NX were more conducive to metabolism of carbohydrate and fat, and healthy growth maintenance in the human body, but mightThese variations suggest that NX rice may be more beneficial for carbohydrate and fat metabolism and overall health maintenance compared to CH. However, it may not be suitable for diabetic patients. YX rice may not be an ideal glycine supplement, rice ofwhile HM, HY, and MX rice could serve as potential lactulose sources. Furthermore, NX and YX rice exhibited higher levels of main storage proteins compared to CH. This study offers valuable insights into the metabolic differences among various rice varieties.

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Effects of storage on brown rice (Oryza sativa L.) metabolites, analyzed using gas chromatography and mass spectrometry

Cited by 18 publications

References 7 publications

ABA Inhibits Rice Seed Aging by Reducing H2O2 Accumulation in the Radicle of Seeds

ABA Inhibits Rice Seed Aging by Reducing H2O2 Accumulation in the Radicle of Seeds

Toxicity of Ethyl Formate to Tribolium castaneum (Herbst) Exhibiting Different Levels of Phosphine Resistance and Its Influence on Metabolite Profiles

Non-targeted GC–MS metabolomics-based differences in Indica rice seeds of different varieties

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