Effects of Storage on Serum Non-Esterified Fatty Acid Concentrations.

Forbes, Allan L.; Camlin, Jane A.

doi:10.3181/00379727-102-25371

Cited by 15 publications

(3 citation statements)

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“…Another factor influencing the results may be the time delay between blood sampling and extraction of the FFA, which in the present investigation was limited to one hour. Storage of serum, even if frozen, is known to cause a considerable and rapid increase in FFA levels (1).…”

Section: Discussionmentioning

confidence: 99%

Plasma Levels of Individual Free Fatty Acids in Patients With Acute Myocardial Infarction

Hagenfeldt

Wester

1973

Journal of Internal Medicine

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Section: Discussionmentioning

confidence: 99%

Plasma Levels of Individual Free Fatty Acids in Patients With Acute Myocardial Infarction

Hagenfeldt

Wester

1973

Journal of Internal Medicine

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“…Discrepancies may result from methodological problems: an increase in FFA levels during storage of sera even in frozen state was reported (Berlin and Oldfelt, 1969;Forbes and Camlin, 1959). We may have avoided this increase by processing sera immediately.…”

Section: Resultsmentioning

confidence: 99%

Thyroid Hormone Binding Inhibition in Critically ill Patients – Who is the Inhibitor?

Schifferdecker

Hering²,

Böhm³

et al. 2009

Exp Clin Endocrinol Diabetes

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In 31 severely ill patients of our intensive care unit an increased thyroid hormone binding inhibition detected by quantitative measurement of the effect of ether extracts of patients' sera on thyroxine tracer binding to TBG was found. As free fatty acids in serum were diminished in these patients, their postulated role as binding inhibitors in the "low T4 syndrome" of critically ill patients must be questioned. It cannot be excluded that THBI assays demonstrating this inhibition measure artefacts caused by heparine-induced in vitro lipolysis.

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“…FA measures were conducted using stored serum samples from the baseline examination (2004–2006) that had been frozen at −80 °C. The samples had not been exposed to any freeze–thaw cycles, and previous literature has documented that serum FA are stable at these temperatures for up to 10 years (Forbes and Camlin, ; Hirsch et al, ). A known amount of heptadecanoic acid (17:0) in FFA, TAG (1,2,3‐triheptadecanoylglycerol), PL (1,2‐diheptadecanoyl‐sn‐glycero‐3‐phosphocholine), and CE (cholesteryl heptadecanoate) forms were added to serum, as an internal standard, prior to extracting total lipids according to the method of Folch et al ().…”

Section: Methodsmentioning

confidence: 99%

The Distribution of Fatty Acid Biomarkers of Dairy Intake across Serum Lipid Fractions: The Prospective Metabolism and Islet Cell Evaluation (PROMISE) Cohort

Santaren

Bazinet

Liu

et al. 2019

Lipids

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Circulating fatty acids (FA) derived largely from dairy consumption have most commonly been measured in total human serum or phospholipid (PL) fractions, and have been used as validated biomarkers of dairy intake in a growing number of epidemiological studies. Nevertheless, measurement and characterization of a wider spectrum of FA biomarkers of dairy across the four major serum lipid fractions is lacking. This study aimed to (1) quantify FA biomarkers of dairy in PL, triacylglycerol (TAG), cholesteryl ester (CE), and unesterified fatty acid (FFA) serum lipid fractions; and (2) identify potential demographic and metabolic factors that may modify the proportions of these FA across serum fractions. Baseline data from 444 adults in the PROMISE cohort were analyzed. FA biomarkers, 15:0, t16:1n‐7, 18:2‐c9,t11, and t18:1n‐7 were quantified from serum. Dairy intake was estimated using the validated Canadian Diet History Questionnaire. Our results show that t18:1n‐7 was the most abundant FA biomarker in all fractions except CE, where 18:2‐c9,t11 was the most abundant. Positive correlations within fractions, and across FA in the PL, CE, and FFA fractions were found, however, TAG FA were negatively correlated with the other fractions. PL and CE FA were positively associated with dairy intake, and negatively associated with markers of dysmetabolism while, in contrast, these markers were predictors of higher TAG dairy FA. This study is the first to demonstrate distinct proportions of dairy FA in different serum lipid fractions. PL and CE FA marked dairy intake in this cohort, while TAG FA appeared to be markers of dysmetabolism.

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Effects of Storage on Serum Non-Esterified Fatty Acid Concentrations.

Cited by 15 publications

References 0 publications

Plasma Levels of Individual Free Fatty Acids in Patients With Acute Myocardial Infarction

Plasma Levels of Individual Free Fatty Acids in Patients With Acute Myocardial Infarction

Thyroid Hormone Binding Inhibition in Critically ill Patients – Who is the Inhibitor?

The Distribution of Fatty Acid Biomarkers of Dairy Intake across Serum Lipid Fractions: The Prospective Metabolism and Islet Cell Evaluation (PROMISE) Cohort

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