Effects of storage temperature on viable bioprosthetic heart valves

Brockbank, Kelvin G.M.; Carpenter, John F.; Dawson, Patti E.

doi:10.1016/0011-2240(92)90058-a

Cited by 45 publications

(21 citation statements)

References 7 publications

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“…13 Conversely, ELS stored at -80°C deteriorated over time, resulting in significant losses in viability and function. These data confirm other findings in either intact tissues (heart valves) 14,6 or single-cell suspensions of bone marrow progenitor cells 15 . In studies on the fibroblast populations of heart valves (found unsuitable for transplantation), Brockbank et al found that those stored for up to 2 years below -135°C maintained activity (glycine incorporation).…”

Section: Discussionsupporting

confidence: 81%

“…It is now generally accepted that for cells to be successfully stored in the long-term, cells and tissues need to achieve ultra-low temperatures below the glass transition of the cell-cryprotectant mixture, so that no mobile water fraction persists in the stored sample. These glasstransition temperatures are typically quoted to be close to -120°C in the types of aqueous media containing DMSO that were used here for cryopreservation of ELS, 14,21 in agreement with our own DSC findings in this study.…”

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confidence: 80%

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Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Massie

Selden²,

Hodgson³

et al. 2013

Tissue Engineering Part C: Methods

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Section: Discussionsupporting

confidence: 81%

supporting

confidence: 80%

Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Massie

Selden²,

Hodgson³

et al. 2013

Tissue Engineering Part C: Methods

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“…101 A series of studies demonstrate that storage below -135°C is necessary for long-term storage of tissues. Brockbank et al 102 studied human allograft heart valve leaflets to evaluate the effect of different storage temperatures ( -80°C and below -135°C) on viability of fibroblasts embedded in the valve. Samples stored at -80°C revealed a time-dependent loss of fibroblast viability.…”

Section: Storage Of Tissuesmentioning

confidence: 99%

Storage of Human Biospecimens: Selection of the Optimal Storage Temperature

Hubel

Spindler

Skubitz

2014

Biopreservation and Biobanking

121

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Millions of biological samples are currently kept at low tempertures in cryobanks/biorepositories for long-term storage. The quality of the biospecimen when thawed, however, is not only determined by processing of the biospecimen but the storage conditions as well. The overall objective of this article is to describe the scientific basis for selecting a storage temperature for a biospecimen based on current scientific understanding. To that end, this article reviews some physical basics of the temperature, nucleation, and ice crystal growth present in biological samples stored at low temperatures (-20°C to -196°C), and our current understanding of the role of temperature on the activity of degradative molecules present in biospecimens. The scientific literature relevant to the stability of specific biomarkers in human fluid, cell, and tissue biospecimens is also summarized for the range of temperatures between -20°C to -196°C. These studies demonstrate the importance of storage temperature on the stability of critical biomarkers for fluid, cell, and tissue biospecimens.

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“…However, in our experience cells typically survive less than 48 h of cold storage in culture media without specially designed hypothermic storage solutions [3]. There are exceptions, such as myofibroblasts in heart valve leaflets [4] or chondrocytes in articular cartilage. There have been reports on chondrocytes in articular cartilage of humans [5][6][7][8][9] and several animal species including rabbits [10], rats [11,12], dogs [13,14], miniature pigs [15], sheep [16], cattle [17,18], and non-human primates [9], demonstrating that chondrocytes survive days or weeks of hypothermic storage in their natural extracellular matrix.…”

Section: Introductionmentioning

confidence: 99%

Impact of Hypothermia upon Chondrocyte Viability and Cartilage Matrix Permeability after 1 Month of Refrigerated Storage

Brockbank

Rahn

Wright

et al. 2011

Transfus Med Hemother

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Background: The purpose of this research was to assess the extracellular matrix and chondrocytes of articular cartilage during refrigerated storage and to determine whether changes could be detected in the time frame that cartilage is stored for clinical use. Methods: Porcine cartilage was stored as either bisected femoral heads with bone attached or plugs without the underlying bone in culture medium with fetal bovine serum for 1 month at 4 °C. Metabolic activity was tested using a resazurin reduction method on intact tissue and viable cell recovery after enzymatic tissue digestion at each time point. Cartilage plug permeability was evaluated by measuring electrical conductivity. Results: Storage in culture medium provided good cartilage viability and metabolic function for 7 days; however, significant changes were observed in femoral heads (p < 0.05). All mean chondrocyte assessment values were <30% of fresh controls at 28 days. Cartilage plugs tended to perform better after 7 days of storage than the femoral heads and retained significantly higher metabolic activity (mean = 94.5% vs. 70.5%; p < 0.05). Cartilage plugs demonstrated consistent changes in electrical conductivity after 28 days of storage (p < 0.05). Conclusion: Refrigerated storage of cartilage results in both loss of chondrocyte viability and matrix permeability.

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Effects of storage temperature on viable bioprosthetic heart valves

Cited by 45 publications

References 7 publications

Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Storage of Human Biospecimens: Selection of the Optimal Storage Temperature

Impact of Hypothermia upon Chondrocyte Viability and Cartilage Matrix Permeability after 1 Month of Refrigerated Storage

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