Effects of Storage Temperature on Indica-Japonica Hybrid Rice Metabolites, Analyzed Using Liquid Chromatography and Mass Spectrometry

Abstract

The Yongyou series of indica-japonica hybrid rice has excellent production potential and storage performance. However, little is known about the underlying mechanism of its storage resistance. In this study, Yongyou 1540 rice (Oryza sativa cv. yongyou 1540) was stored at different temperatures, and the storability was validated though measuring nutritional components and apparent change. In addition, a broad-targeted metabolomic approach coupled with liquid chromatography-mass spectrometry was applied to analyze the metabolite changes. The study found that under high temperature storage conditions (35 °C), Yongyou 1540 was not significantly worse in terms of fatty acid value, whiteness value, and changes in electron microscope profile. A total of 19 key differential metabolites were screened, and lipid metabolites related to palmitoleic acid were found to affect the aging of rice. At the same time, two substances, guanosine 3′,5′-cyclophosphate and pipecolic acid, were beneficial to enhance the resistance of rice under harsh storage conditions, thereby delaying the deterioration of its quality and maintaining its quality. Significant regulation of galactose metabolism, alanine, aspartate and glutamate metabolism, butyrate metabolism, and arginine and proline metabolism pathways were probably responsible for the good storage capacity of Yongyou 1540.

Metabolic mechanism of nitrogen modified atmosphere storage on delaying quality deterioration of rice grains

“…4-aminobutyric acid (GABA) can improve the resistance of rice seeds to unfavorable storage environment and delay the aging process of seeds ( Zhu et al, 2022 ). It was also reported that GABA may accumulated under adverse environments ( Chang et al, 2020 ).…”

Molecular Research for Cereal Grain Quality

“…This Special Issue aims to provide a forum on the most recent advances in the application of molecular tools to understand the mechanism for improving any cereals’ grain quality. A total of 13 papers were collected for this Special Issue [ 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ], mainly covering rice and wheat crops.…”

“…The storage of rice is also an important part of its production and transactions, and only with good storage performance can its commercial value be maintained in commodity transactions. Zhu et al [ 12 ] found that under high-temperature storage conditions (35 °C), the indica–japonica hybrid rice Yongyou 1540 was not significantly worse in terms of fatty acid value, whiteness value, and changes in electron microscope profile. Metabolomics analysis identified 19 key differential metabolites, in which the lipid metabolites related to palmitoleic acid were found to affect the aging of rice.…”