Effects of Storage Time and Freezing Temperature on Clinical Chemical Parameters From Canine Serum and Heparinized Plasma

Thoresen, S. I.; Tverdal, Aage; Havre, Gustav N.; Morberg, Hanne

doi:10.1111/j.1939-165x.1995.tb00954.x

Cited by 81 publications

(85 citation statements)

References 19 publications

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“…However, we stored the samples frozen for 8-12 months. This can influence the enzyme values that can decrease during storage (Hunter & Madin, 1978;Thoresen et al, 1995;Tryland & Brun, 2001;Tryland et al, 2002) which could partly explain the lower values observed in this study.…”

Section: Discussionmentioning

confidence: 40%

Haematological and serum biochemical reference values in free-ranging red deer (Cervus elaphus atlanticus)

Rosef

Nystøyl

Solenes

et al. 2004

Ran

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Analyses of haematological and biochemical constituents were carried out on the Norwegian subspecies of free-ranging red deer (Cervus elaphus atlanticus). All animals were captured from January to March by using a mixture of xylazine and tiletamin-zolazepam. Immobilisation was performed with plastic projectile syringes fired from a dart gun. Fourteen haematological parameters were analysed. There were no differences in the values between hinds and stags and between adults and calves (P > 0.01). Of the 22 biochemical compounds investigated there was a significant difference (P < 0.01) between calves and adults for lactate dehydrogenase (LD), globulin, beta globulin, gamma globulin, and the minerals Na, K, Mg, Zn, Ca, and P. Differences (P < 0.01) between hinds and stags were found in cholesterol, gamma glutamyl transferase (GGT), alpha-1 globulin, alpha-2 globulin and Cu. The blood values determined in this study can be used as reference values for this red deer subspecies immobilised with a mixture of xylazine-tiletamin-zolazepam for health control and diagnosis of diseases.

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Section: Discussionmentioning

confidence: 40%

Haematological and serum biochemical reference values in free-ranging red deer (Cervus elaphus atlanticus)

Rosef

Nystøyl

Solenes

et al. 2004

Ran

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“…Samples frozen for a shorter period of time appeared to have less difference than those samples frozen for longer periods. These results show that freezing can detrimentally affect TP concentrations, especially during longer freezing periods, as other studies have shown in different species [22] or with other blood analytes [23].…”

Section: Discussionsupporting

confidence: 55%

Differences in Total Protein Concentration between Fresh and Frozen Serum and Plasma Samples Used to Assess Failure of Passive Transfer in Dairy Calves

Miller¹

2014

Adv Dairy Res

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The objectives of this study were to determine differences in total protein (TP) concentration between samples before and after freezing, and to determine the correlation between total protein in serum and plasma to evaluate passive transfer in calves. Blood was collected via jugular vein puncture from a total of 127 calves (34 Holstein and 93 Jersey) once between 1 and 3 days of age. Serum and plasma protein concentrations were measured via digital refractometer in fresh specimens; samples were then frozen for storage. After a variable period of time in storage (≤1 to 8 months), frozen samples were thawed and re-analyzed. Passing-Bablok regression showed that total protein concentration in plasma was consistently higher than in serum. Additionally, multivariate regression showed that Jersey calves had higher total protein concentration in serum than Holstein calves (0.429 ± 0.049 g/ dL), and that each additional month of storage resulted in lower protein concentration in serum (-0.116 ± 0.017 g/ dL). Electrophoresis showed that all protein fraction levels varied after long-term storage of serum, although albumin accounted for most of the variation. It is concluded that plasma samples can be used as a new and improved option to evaluate effectiveness of colostrum management protocols under field conditions because plasma samples are easier to handle in the field and they correlate well with fresh serum total protein concentration and are not significantly affected by frozen storage.

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“…In contrast, other parameters, showed clear differences in the type (ALT, AST) or magnitude (ALKP) of age-related change between the species, demonstrating the importance of determining normal values for different age groups of each species. Although biochemical parameters assayed from stored samples should be interpreted with care (Thoresen et al, 1995), our results provide values for both clinically normal adult and juvenile kori and houbara bustards which may prove useful for the interpretation of some laboratory findings in clinical cases.…”

Section: Discussionmentioning

confidence: 99%

Age-Related Plasma Chemistry Changes in Houbara and Kori Bustards in the United Arab Emirates

Bailey

Wernery

Howlett

et al. 1999

Journal of Wildlife Diseases

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Plasma chemistry parameters were measured in adult (Ͼ1.5 yr) and juvenile (4-8 and 9-16 wk) captive houbara bustards (Chlamydotis undulata macqueenii) and from adult (Ͼ1.5 yr) and juvenile (4-8, 9-16, 17-24, 25-32, 33-40 and 41-52 wk) captive kori bustards (Ardeotis kori) to study age-related changes. A comparison of the values obtained was made between adult and juvenile bustards of both species and from the literature with other bustard species. Significant differences between adult and juvenile bustards of both species were found for glucose, total protein, alkaline phosphatase (ALKP), aspartate amino transferase (AST), lactate dehydrogenase, and calcium. Some parameters, such as calcium, showed comparable age-related changes in both species. In contrast, other parameters showed clear differences in the type (ALT, AST) or magnitude (ALKP) of age-related change between the species, demonstrating the importance of determining normal values for individual species. The results obtained from this study provide blood chemistry values for these species and demonstrate age-related differences between adult and juvenile birds.

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Effects of Storage Time and Freezing Temperature on Clinical Chemical Parameters From Canine Serum and Heparinized Plasma

Cited by 81 publications

References 19 publications

Haematological and serum biochemical reference values in free-ranging red deer (Cervus elaphus atlanticus)

Haematological and serum biochemical reference values in free-ranging red deer (Cervus elaphus atlanticus)

Differences in Total Protein Concentration between Fresh and Frozen Serum and Plasma Samples Used to Assess Failure of Passive Transfer in Dairy Calves

Age-Related Plasma Chemistry Changes in Houbara and Kori Bustards in the United Arab Emirates

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