Effects of structural modification on gene transfection and self-assembling properties of amphiphilic dendrimers

Guillot, M; Eisler, Sara; Weller, Kathrin; Merkle, Hans P.; Gallani, Jean‐Louis; Diederich, François

doi:10.1039/b518174g

Cited by 49 publications

(26 citation statements)

References 37 publications

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“…[2] DNA condensation can be induced by nearly all kinds of gene delivery systems ranging from viral gene delivery systems [3,4] to nonviral gene 726 S. Hou et al delivery systems, such as cationic lipids, [5] gemini surfactants, [6] and cationic polymers. [7] The interactions between DNA and gene delivery vectors establish some correlations between the DNA condensation process and gene transfection efficiency, [8] for instance, the dimension of complexes, the ratio of amino group in polymer to the phosphate group in DNA, and the charge of polymer influence gene transfection efficiency. [9,10] Much effort has been expanded to optimize the DNA condensation efficiency of gene delivery vectors to obtain better gene delivery efficiency, such as reducing the number of positive charges, [11] conjugating poly(ethylene glycol) (PEG) chains, [12] decreasing the polymer molecular mass, [13] etc.…”

Section: Introductionmentioning

confidence: 99%

Method to Improve DNA Condensation Efficiency by Alkali Treatment

Hou

Feng

2009

Nucleosides, Nucleotides and Nucleic Acids

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The improvement of DNA's bioactivities by altering their structure is meaningful for their biological applications, ranging from DNA condensation study to gene therapeutic research. In this study, we treated the plasmid DNA with alkali and investigated the structure and the condensation efficiency of the alkali-treated DNA. We noticed that the alkali treatment could significantly increase the DNA condensation efficiency with spermidine and polyethylenimine (PEI). In addition, due to the improved interactions between the alkali-treated DNA and PEI, gene transfection experiments could be performed in the presence of less PEI. This research can contribute to the creation of a universal method to enhance the interaction between DNA and gene delivery vectors by alkali treatment, and should have significant potential in the field of gene therapy.

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Section: Introductionmentioning

confidence: 99%

Method to Improve DNA Condensation Efficiency by Alkali Treatment

Hou

Feng

2009

Nucleosides, Nucleotides and Nucleic Acids

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“…Janus dendrimer consists of two different segments, a hydrophilic and a hydrophobic part. The commonly found hydrophilic components in Janus dendrimers are oligoethylene oxide, D‐mannose, D‐galactose, D‐lactose, hydroxyl terminated dendron and poly(amido amine) (PAMAM) dendrons . The commonly used hydrophobic components are poly(aryl ether) dendrons and alkyl chains .…”

Section: Introductionmentioning

confidence: 99%

Janus Dendrimer from Poly(Aryl Ether) Linked PAMAM for Supergelation and Guest Release

Prabakaran

Prasad

2016

ChemistrySelect

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The present work reports an easy-to-make procedure of Janus dendrimers containing two most widely used dendrons [poly (aryl ether) and poly(amido amine) {PAMAM}], and utilize them, for the first time, to form a hydrogel with supergelation ability. The first generation (G 1 G 1 ) Janus dendrimer forms hydrogel with a critical gelation concentration (CGC) of 6.5 mg/mL, whereas the CGC for G 1 G 1.5 dendrimer went as low as 0.90 mg/ mL in DMSO-H 2 O mixture. Judicious choice of solvent medium (ethanol-water) led to the formation of flexible morphology (long fibres to vesicles) by the Janus dendrimers, suggesting the possibility of tuning the mechanism of self-assembly in the system. The dye (rhodamine B) loading efficiency in the gel was successfully carried out using different generations {G 1 G 1 and G 1 G 1.5 } of the Janus dendrimer. Generation G 1 G 1 hydrogel (1 wt%) showed maximum dye loading efficiency of 4 mg/mL. Rhodamine B release profiles from the Janus dendrimers were determined and the results indicate that slow release kinetics was observed in the case of G 1 G 1 , where the self-assembly is robust.

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“…[1][2][3][4][5] However, PAMAM toxicity profiles are problematic for biomedical applications because of the presence of polycationic substituents, and their persistence in cells. [8][9][10][11][12][13][14] In addition, this type of dendrimers combing the characteristics of cationic polymers and lipids, can give rise to synergistic effects in gene delivery. 6,7 This supramolecular strategy is a novel concept in the field of dendrimer-mediated nucleic acid delivery.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 This supramolecular strategy is a novel concept in the field of dendrimer-mediated nucleic acid delivery. [8][9][10][11][12][13][14] In addition, this type of dendrimers combing the characteristics of cationic polymers and lipids, can give rise to synergistic effects in gene delivery.…”

Section: Introductionmentioning

confidence: 99%

Self‐aggregation of amphiphilic [60]fullerenyl focal point functionalized PAMAM dendrons into pseudodendrimers: DNA binding involving dendriplex formation

Hung

Chang

Liu

et al. 2014

J Biomedical Materials Res

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In this research, we successfully performed a "click" synthesis of amphiphilic poly(amido amine) dendron-bearing fullerenyl conjugate (C60 G1 ) using a copper(I)-catalyzed azide-alkyne cycloaddition reaction. The strong hydrophobicity of the C60 moiety induces self-assembly of C60 G1 into core-shell-like "pseudodendrimers" with a uniform size distribution and positively charged peripherals. The pseudodendrimers were well-characterized by atomic force microscopy (AFM), transmission electron microscopy, and dynamic light scattering. On the basis of electrostatic interactions, the polycationic C60 G1 assembly can serve as a nonviral gene vector. An ethidium bromide displacement assay and agarose gel electrophoresis both indicated that C60 G1 assembly forms stable complexes with the cyclic reporter gene (pEGFP-C1) at low nitrogen-to-phosphorous (N/P) ratios. AFM analysis revealed a dynamic complex-formation process, and confirmed the synthesis of C60 G1 /pEGFP-C1 hybrids with a particle dimensions less than 200 nm. Fluorescence microscopy and flow cytometry revealed that 51% of HeLa and 43% of MCF-7 cells are positive to the YOYO-1-labeled hybrids at an N/P ratio of 2, being comparable to TurboFect-mediated delivery.

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Effects of structural modification on gene transfection and self-assembling properties of amphiphilic dendrimers

Abstract: A library of novel amphiphilic, self-assembling dendrimers was designed and synthesised to evaluate the effects of structural changes on transfection efficiency.

Cited by 49 publications

References 37 publications

Method to Improve DNA Condensation Efficiency by Alkali Treatment

Method to Improve DNA Condensation Efficiency by Alkali Treatment

Janus Dendrimer from Poly(Aryl Ether) Linked PAMAM for Supergelation and Guest Release

Self‐aggregation of amphiphilic [60]fullerenyl focal point functionalized PAMAM dendrons into pseudodendrimers: DNA binding involving dendriplex formation

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