Effects of sub-lethal doses of miconazole nitrate on Labeo rohita and its curing efficacy against Saprolegniasis

Singh, Mukta; Saha, Ratan K.; Saha, Himadri; Sahoo, Abinash; Biswal, Auroshree

doi:10.1016/j.aquaculture.2018.04.029

Cited by 5 publications

(3 citation statements)

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“…There were 32 treatments (T1—6.30 mgMCZ kgBW −1 , T2—12.61 mgMCZ kgBW −1 , T3—25.22 mgMCZ kgBW −1 and control—0.00 mgMCZ kgBW −1 ) combined with eight exposure times (0, 6, 12, 24, 48, 96, 120 and 240 hr) each in triplicate. A total of 480 fingerlings were distributed randomly in 96 glass aquariums of 45 l capacity (five fingerlings each) following completely randomized design as described in our earlier studies (Singh et al., ). The purpose of taking separate tanks for each sampling hours is to nullify the possibilities of effect of handling stresses arises due to the repeated sampling from a single tank at hourly intervals.…”

Section: Methodsmentioning

confidence: 99%

“…The experiment was carried out to evaluate the preventive efficacy of best MCZ curing dose (Singh et al., ) against S. parasitica infection in L. rohita fingerlings. In this experiment, rohu fingerlings (average length 16.7 ± 1.5 cm; weight 24.2 ± 1.5 g) were fed with best experimental diet of 25.2 mg MCZ kgBW −1 on first day and were challenged with oomycetes zoospores on 2nd, 4th and 12th day of drug administration.…”

Section: Methodsmentioning

confidence: 99%

“…Four iso-nitrogenous (35.18%-35.99% crude protein) and iso-caloric (357-365 kcal 100 g −1 ) diets with purified ingredients (Table 1) were formulated. Lethal dose 50% (LD 50 ) of MCZ for rohu fingerlings was found to be 126.14 mgMCZ kgBW −1 (Singh, Saha, Saha, Sahoo, & Biswal, 2018). Three purified test diets were prepared following Saha, Pal, Sahu, Saha, and Goswami (2017) with the addition of calculated amount of sublethal dose of MCZ (Himedia Laboratories, Mumbai, India) as T1 (6.30 mgMCZ kgBW −1 ), T2 (12.61 mgMCZ kgBW −1 ) and T3 (25.22 mgMCZ kgBW −1 ) by manipulating the cellulose level.…”

Section: Experimental Diets and Feeding Ratementioning

confidence: 99%

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Effect of miconazole nitrate on immunological response and its preventive efficacy in Labeo rohita fingerlings against oomycetes Saprolegnia parasitica

Singh¹,

Saha²,

Saha³

et al. 2018

Journal of Fish Diseases

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This study evaluated the effect of sublethal doses of antifungal drug miconazole nitrate (MCZ) on immunological responses and its role as a prophylactic drug against S. parasitica in Labeo rohita fingerlings. Fish were fed with sublethal doses of MCZ, that is, T1-6.30 mgMCZ kgBW , T2-12.61 mgMCZ kgBW and T3-25.22 mgMCZ kgBW , and sampling was done at different time intervals for 240 hr. Immunological parameters viz. lysozyme activity, oxygen radical production and plasma antiprotease activity showed significant enhancement (p < 0.05) in fish fed with T2 and T3 doses. Expression of immune-relevant genes such as TLR-22 and β2-M showed significantly higher expression at 6 hr and 24 hr of sampling in both liver and head kidney. However, these genes showed a downregulation after 120 hr of sampling in both the tissues. Preventive efficacy study showed that single dose of MCZ provides protection against oomycetes up to the fourth day of infection. Significantly higher mortality was observed in control diet-fed fish as compared to fish fed with MCZ medicated diet. Thus, it can be concluded that the MCZ can act as a potent antifungal agent for preventing oomycetes infection as well as to enhance the immune response.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%