Effects of Subclinical <i>Mycobacterium chelonae</i> Infections on Fecundity and Embryo Survival in Zebrafish

Kent, Michael L.; Watral, Virginia; Kirchoff, Nicole; Spagnoli, Sean; Sharpton, Thomas J.

doi:10.1089/zeb.2015.1204

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…In contrast, Kent et al. (2016) found no correlation between infection status, number of eggs, or viability of offspring from parental zebrafish infected with M. chelonae .…”

Section: Discussionmentioning

confidence: 94%

Differences in susceptibility to Mycobacterium chelonae in zebrafish (Danio rerio) lines commonly used in scientific research

Janik

Whipps

2021

Journal of Fish Diseases

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Mycobacteriosis is one of the most common diseases encountered in laboratory zebrafish. These infections can present a problem to researchers using zebrafish because they may introduce unknown experimental variables. Whilst differences in severity of infections between species of Mycobacterium infecting zebrafish have been well documented, little is known about differences in susceptibility between zebrafish lines. Previous surveys have found higher prevalence in the TU zebrafish line relative to other lines, suggesting that there may be underlying genetic differences in susceptibility. This study investigates Mycobacterium chelonae H1E2‐GFP infections in four different zebrafish lines commonly used in research (AB, 5D, casper and TU). Fish were exposed to a labelled (green‐fluorescent protein (GFP)) strain of M. chelonae by intraperitoneal injection, and infection status was evaluated after 10 weeks. Visualization of GFP in euthanized fish and histology were used as endpoints. In GFP images, severity was assessed by image analysis, and in histological sections, counts of granulomas containing acid‐fast bacteria were used. Results indicated differences in severity of infections between lines, but no significant differences in prevalence.

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“…In contrast, Kent et al. (2016) found no correlation between infection status, number of eggs, or viability of offspring from parental zebrafish infected with M. chelonae .…”

Section: Discussionmentioning

confidence: 94%

Differences in susceptibility to Mycobacterium chelonae in zebrafish (Danio rerio) lines commonly used in scientific research

Janik

Whipps

2021

Journal of Fish Diseases

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“…39 Investigators may not be fully aware of the potential impact of underlying disease on their research when postlarval zebrafish are used in medium-or long-term experiments. 25,26,[40][41][42] For example, the infection of P. neurophilia could compromise developmental neurotoxicity testing and musculoskeletal development studies in zebrafish. 8 It would likely occur in experiments with growth and fecundity endpoints.…”

Section: Discussionmentioning

confidence: 99%

Zebrafish Health Conditions in the China Zebrafish Resource Center and 20 Major Chinese Zebrafish Laboratories

Liu

Pan

et al. 2016

Zebrafish

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In China, the use of zebrafish as an experimental animal in the past 15 years has widely expanded. The China Zebrafish Resource Center (CZRC), which was established in 2012, is becoming one of the major resource centers in the global zebrafish community. Large-scale use and regular exchange of zebrafish resources have put forward higher requirements on zebrafish health issues in China. This article reports the current aquatic infrastructure design, animal husbandry, and health-monitoring programs in the CZRC. Meanwhile, through a survey of 20 Chinese zebrafish laboratories, we also describe the current health status of major zebrafish facilities in China. We conclude that it is of great importance to establish a widely accepted health standard and health-monitoring strategy in the Chinese zebrafish research community. Introduction In China, zebrafish-related research started in the early 1990s, and the research community has been rapidly growing since 2000. From 2000 to 2014, the zebrafish-related research outputs have increased more than 50 times in China, while the proportion of zebrafish-related articles published by Chinese investigators in the total articles published worldwide has increased from 1.5% to 16.8%. 1,2To meet the growing needs of zebrafish strains and zebrafish technical services, the China Zebrafish Resource Center (CZRC) was established at the Institute of Hydrobiology, Chinese Academy of Sciences in Wuhan, in October 2012. Till late 2015, the CZRC has maintained more than 900 lines of wild-type, mutant, and transgenic zebrafish lines and a number of DNA plasmids and cell lines.In 2013, 40 Chinese zebrafish laboratories started the Zebrafish All Gene Knockout Project for chromosome 1 (ZKO Project). The project planned to knock out more than 1000 genes on zebrafish chromosome 1 using the CRISPR/Cas9 technique. As the project coordinator, the CZRC is in charge of the preservation and distribution of all the knockout lines built in the project, and this enables the CZRC to know the status of zebrafish facilities in the major Chinese zebrafish laboratories.In this article, we introduce the aquatic infrastructure design, daily management, fish husbandry, and the healthmonitoring programs in CZRC, and the current health status of 20 zebrafish facilities in China. Materials and Methods Equipments used in fish facilityThe CZRC is equipped with two water purification systems (ESEN-AW-JH), provided by the Beijing Enviromental Science company. The main fish room is equipped with six independent sets of recirculating aquatic system, in which five sets (ESEN-AW-RC12) were provided by Beijing ESEN, and one set (WCFE6/8/12, ZC-D5) was provided by Shanghai Hai Sheng Biotech company. The quarantine room is equipped with four independent flow-through systems (ESEN-AW-DU5SS), which were provided by Beijing ESEN. The nursery room is equipped with three recirculating racks, in which two racks (ESEN-AW-DU5SS) were provided by Beijing ESEN and one rack (Z-A-S5) was provided by Shanghai Hai Sheng. The food p...

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“…10 This parasite has been detected by using PCR on sperm stripped from intact fish and dissected testes, 11 and M. chelonae is often observed in ovaries and testes of zebrafish. 12,13 ZIRC categorizes Mycobacterium marinum, Mycobacterium haemophilum, Edwardsiella ictaluri, and Pseudocapillaria tomentosa as high-risk pathogens due to their ability to cause severe infections and high rates of mortality. 4 M. haemophilum has been observed in the ovaries and testes of infected fish.…”

Section: Introductionmentioning

confidence: 99%

Survival of Bacterial and Parasitic Pathogens from Zebrafish (Danio rerio) After Cryopreservation and Thawing

2018

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Cryopreservation is a common method used to preserve the sperm of various animal species, and it is widely used with zebrafish (Danio rerio). As with other animals, there is a possibility of paternal pathogen transmission through sperm. We evaluated the ability of five common and important pathogens of zebrafish to survive cryopreservation as used with zebrafish sperm and freezing without cryopreservant. We evaluated Mycobacterium chelonae, Mycobacterium marinum, and Edwardsiella ictaluri, each originally isolated from zebrafish, eggs of Pseuodocapillaria tomentosa, and spores of Pseudoloma neurophilia. Each mycobacterial isolate showed relatively minimal reduction in survival after freezing and thawing, particularly when subjected to cryopreservation. E. ictaluri also showed survival after cryopreservation, but exhibited a several log reduction after freezing at -80°C without cryopreservant. With P. neurophilia, two separate experiments conducted 3 years apart yielded very similar results, showing some, but reduced, survival of spores by using three different viability assays: SYTOX stain, Fungi-Fluor stain, and presence of a spore vacuole. Eggs of P. tomentosa showed no survival based on larvation of eggs when subjected to either freezing method. Given that four of the five pathogens exhibited survival after cryopreservation, we recommend that sperm samples or donor male zebrafish fish be tested for pathogens when sperm are to be stored by using cryopreservation.

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Effects of Subclinical Mycobacterium chelonae Infections on Fecundity and Embryo Survival in Zebrafish

Cited by 8 publications

References 15 publications

Differences in susceptibility to Mycobacterium chelonae in zebrafish (Danio rerio) lines commonly used in scientific research

Differences in susceptibility to Mycobacterium chelonae in zebrafish (Danio rerio) lines commonly used in scientific research

Zebrafish Health Conditions in the China Zebrafish Resource Center and 20 Major Chinese Zebrafish Laboratories

Survival of Bacterial and Parasitic Pathogens from Zebrafish (Danio rerio) After Cryopreservation and Thawing

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