2012
DOI: 10.1007/s00244-012-9849-1
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Effects of Sublethal Dose of Fipronil on Neuron Metabolic Activity of Africanized Honeybees

Abstract: Fipronil is a neurotoxic insecticide that inhibits the gamma-aminobutyric acid receptor and can affect gustative perception, olfactory learning, and motor activity of the honeybee Apis mellifera. This study determined the lethal dose (LD50) and the lethal concentration (LC50) for Africanized honeybee and evaluated the toxicity of a sublethal dose of fipronil on neuron metabolic activity by way of histochemical analysis using cytochrome oxidase detection in brains from worker bees of different ages. In addition… Show more

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Cited by 46 publications
(39 citation statements)
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“…The acute treatment and sub-chronic treatment (D-I) have the same structure and morphology of mushroom bodies from control group. and Meixner (2009) and Roat et al (2013). The effects observed in the optic lobes may be due to direct toxicity of thiamethoxam, or this effect may result from the changes observed in the larval development.…”
Section: Discussionmentioning
confidence: 93%
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“…The acute treatment and sub-chronic treatment (D-I) have the same structure and morphology of mushroom bodies from control group. and Meixner (2009) and Roat et al (2013). The effects observed in the optic lobes may be due to direct toxicity of thiamethoxam, or this effect may result from the changes observed in the larval development.…”
Section: Discussionmentioning
confidence: 93%
“…Morphological studies to assess the effects of insecticides in target and non-target organs have been used as a parameter to predict the risks that these compounds may pose (Malaspina and Silva-Zacarin, 2006;Cruz et al, 2010;Roat et al, 2013;Oliveira et al, 2013). In the present study, the optic lobes of Africanized honeybee larvae exposed to a sublethal concentration of thiamethoxam exhibited alterations that were evidenced by immunocytochemical and morphological techniques, and TUNEL reactions confirmed that condensed cells were undergoing cell death process.…”
Section: Discussionmentioning
confidence: 99%
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“…The honeybee toxicity assay was performed using a sublethal dose of fipronil that was based on the LD 50 value for newly emerged honeybees, which was determined by Roat et al (2013) to be 1.06 ng fipronil per bee. Fipronil (95.0 %, analytical grade) was supplied by Dalian Raiser Pesticides (China).…”
Section: Honeybee Toxicity Assaysmentioning
confidence: 99%
“…The results from studies of the oral toxicity of fipronil against the stingless bee Melipona scutellaris showed that these insects exhibited signs of intoxication, such as initial tremors followed by paralysis, before their death (Lourenço et al 2012). It was reported that low doses of fipronil impaired the olfactory learning (0.5 ng/bee) and memory abilities (0.1 ng/bee) in A. mellifera through the action of multiple targets, including glutamatergic and GABAergic inhibitory signaling (El Hassani et al 2005) Roat et al (2013) reported the lethal dose (LD 50 ) and lethal concentration (LC 50 ) values of fipronil for newly emerged Africanized honeybees showing that this insecticide may be harmful to these bees independently of exposure route: LD 50 = 1.06 ng fipronil/bee and LC 50 = 1.27 ng fipronil/lL of food, respectively. Besides, this study assessed the side effect of a sublethal dose of fipronil on neuron metabolic activity from Africanized honeybees and showed that the insecticide causes metabolic changes by increasing the respiratory activity of mitochondria.…”
Section: Introductionmentioning
confidence: 98%