Effects of sulfated hyaluronan on keratinocyte differentiation and Wnt and Notch gene expression

Nagira, Tsutomu; Nagahata-Ishiguro, Misao; Tsuchiya, Toshie

doi:10.1016/j.biomaterials.2006.09.041

Cited by 23 publications

(15 citation statements)

References 30 publications

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“…2). It was reported that the NHEK proliferation was down-regulated when they were cultured on the sulfated HA coated surface (Nagira et al, 2007). In addition, the increase in K1 gene expression was found in the same study, which is consistent with our observation.…”

Section: Discussionsupporting

confidence: 82%

Chitosan Increases α6 Integrin^high/CD71^highHuman Keratinocyte Transit-Amplifying Cell Population

Shin¹,

Shim²,

Kim³

et al. 2010

Biomolecules and Therapeutics

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-Glycosaminoglycans (GAGs) and chitosan have been used as matrix materials to support the dermal part of skin equivalent which is used for both pharmacological and toxicological evaluations of drugs potentially used for dermatological diseases. However, their biological roles of GAGs and chitosan in the skin equivalent are still unknown. In the present study, we evaluated whether GAGs and chitosan directly affect keratinocyte stem cells (KSCs) and their transit-amplifying cells (TA cells). Among supporting matrix materials, chitosan significantly increased the number of α6 integrin high /CD71 high human keratinocyte TA cells by 48.5%.In quantitative real-time RT-PCR analysis, chitosan significantly increased CD71 and CD200 gene transcription whereas not α6 integrin. In addition, the level of the gene transcription of both keratin 1 (K1) and K10 in the chitosan-treated human keratinocytes was significantly lower than those of control, suggesting that chitosan inhibit keratinocyte differentiation. We also found that N-acetyl-D-glucosamine (NAG) and β-(1-4)-linked D-glucosamine (D-glc), two components of chitosan, have no effect on the expression of CD71, K1, and K10, suggesting that each monomer component of chitosan is not enough to regulate the number of epidermal keratinocyte lineage. Conclusively, chitosan increases keratinocyte TA cell population which may contribute to the cellular mass expansion of the epidermal part of a skin equivalent system.

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Section: Discussionsupporting

confidence: 82%

Chitosan Increases α6 Integrin^high/CD71^highHuman Keratinocyte Transit-Amplifying Cell Population

Shin¹,

Shim²,

Kim³

et al. 2010

Biomolecules and Therapeutics

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“…However, this study suggests that an enhancement of differentiation level of NHOsts induced by phosphorylated or sulfate group on the surface is triggered by a direct interaction between the chemical group and NHOsts, followed by signal cascades in which GJIC does not participate. In fact, sulfated polysaccharides have been reported to affect expression of several genes relating to cell differentiation, 15 but a mechanism of the sulfated group to affect the expression of these genes remains to be clarified. The details of the interaction and the signal cascades will be clarified in future studies.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies of cell behavior on SAM surfaces with systematic patterns from hydrophobic and hydrophilic groups have been reported, but studies on an effect of specific functional groups such as amino (positively charged group), phosphate (possible site to initiate hydroxyapatite nucleation), and sulfate groups are not easily found although these groups can be expected to affect cell behavior via interaction with endogenous growth factors. We have been working on this and have reported that polysaccharides modified with sulfated groups enhance differentiation level of interacted cells in vitro ,15 suggesting a preferable property of sulfated groups on biocompatibility of materials. Therefore, studies on an interaction between a specified functional group such as a sulfated group and cells will satisfy one of our interests in mechanisms of sulfated groups on cell fate, and give valuable information of key factors regulating biocompatibility of various biomaterials.…”

Section: Introductionmentioning

confidence: 99%

Effects of surface chemistry prepared by self‐assembled monolayers on osteoblast behavior

Nakaoka

Yamakoshi

Isama

et al. 2010

J Biomedical Materials Res

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A surface of biomaterials is known to affect the behavior of cells after their adhesion on the surface, indicating that surface characteristics of biomaterials play an important role in cell adhesion, proliferation, and differentiation. To assess the effects of functional groups on biomaterial surface, normal human osteoblasts (NHOsts) were cultured on surfaces coated with self-assembled monolayers (SAMs) containing various functional groups, and the adhesion, proliferation, differentiation, and gap junctional intercellular communication (GJIC) of the NHOsts were investigated. In the case of SAM with terminal methyl groups (hydrophobic surface), NHOst adhesion and proliferation was less prevalent. In contrast, NHOsts were adhered well on SAMs with hydroxyl, carboxyl, amino, phosphate, and sulfate group, which are relatively hydrophilic, their proliferation and differentiation level were dependent on the type of functional groups. Especially, when they were cultured on either SAMs with phosphate or sulfate group, both their alkaline phosphate activity and the calcium deposition by them were enhanced more than those cultured on a collagen-coated dish. More interestingly, GJIC of NHOsts, which has been reported to play a role in cell differentiation as well as homeostasis of cells, were not significantly different among the SAM surfaces tested. These suggest that a specific functional group on a material surface can regulate NHOst adhesion, proliferation, and differentiation via cell-functional group interaction without influencing their homeostasis.

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“…These sulfated polymers have been shown to bind proteins with high affinity and when delivered in vivo significantly enhance tissue repair, presumably by replacing degraded heparin and heparan sulfate and increasing the bioavailability of proteins 15 . While HA is unique among GAGs in that it lacks sulfate groups, researchers have sulfated HA through nucleophilic substitution of primary hydroxyl hydrogens on HA with SO 3 by simply reacting SO 3 complexes with HA in an organic solvent 16–18 . Reaction of an SO 3 /DMF complex with HA results in a specific sulfation pattern that binds heparin-binding proteins (HBPs) with a high affinity 17, 19 .…”

Section: Introductionmentioning

confidence: 99%

Incorporation of sulfated hyaluronic acid macromers into degradable hydrogel scaffolds for sustained molecule delivery

et al. 2014

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Synthetically sulfated hyaluronic acid (HA) has been shown to bind proteins with high affinity through electrostatic interactions. While HA-based hydrogels have been used widely in recent years for drug delivery and tissue engineering applications, incorporation of sulfated HA into these networks to attenuate the release of proteins has yet to be explored. Here, we developed sulfated and methacrylate-modified HA macromers and incorporated them into HA hydrogels through free radical-initiated crosslinking. The sulfated HA macromers bound a heparin-binding protein (i.e., stromal cell-derived factor 1-α, SDF-1α) with an affinity comparable to heparin and did not alter the gelation behavior or network mechanics when copolymerized into hydrogels at low concentrations. Further, these macromers were incorporated into electrospun nanofibrous hydrogels to introduce sulfate groups into macroporous scaffolds. Once incorporated into either uniform or fibrous HA hydrogels, the sulfated HA macromers significantly slowed encapsulated SDF-1α release over 12 days. Thus, these macromers provide a useful way to introduce heparin-binding features into radically-crosslinked hydrogels to alter protein interactions for a range of applications.

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Effects of sulfated hyaluronan on keratinocyte differentiation and Wnt and Notch gene expression

Cited by 23 publications

References 30 publications

Chitosan Increases α6 Integrin^high/CD71^highHuman Keratinocyte Transit-Amplifying Cell Population

Chitosan Increases α6 Integrin^high/CD71^highHuman Keratinocyte Transit-Amplifying Cell Population

Effects of surface chemistry prepared by self‐assembled monolayers on osteoblast behavior

Incorporation of sulfated hyaluronic acid macromers into degradable hydrogel scaffolds for sustained molecule delivery

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Effects of sulfated hyaluronan on keratinocyte differentiation and Wnt and Notch gene expression

Cited by 23 publications

References 30 publications

Chitosan Increases α6 Integrinhigh/CD71highHuman Keratinocyte Transit-Amplifying Cell Population

Chitosan Increases α6 Integrinhigh/CD71highHuman Keratinocyte Transit-Amplifying Cell Population

Effects of surface chemistry prepared by self‐assembled monolayers on osteoblast behavior

Incorporation of sulfated hyaluronic acid macromers into degradable hydrogel scaffolds for sustained molecule delivery

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Chitosan Increases α6 Integrin^high/CD71^highHuman Keratinocyte Transit-Amplifying Cell Population

Chitosan Increases α6 Integrin^high/CD71^highHuman Keratinocyte Transit-Amplifying Cell Population