Effects of Sulfide Flavors on AHL‐Mediated Quorum Sensing and Biofilm Formation of <i>Hafnia alvei</i>

Hou, Hongman; Wang, Yifang; Zhang, Gongliang; Zhu, Yaolei; Long-quan, XU; Hao, Hongshun; Wang, Yue; Meishan, Lu

doi:10.1111/1750-3841.14345

Cited by 14 publications

(14 citation statements)

References 42 publications

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“…The stainless steel was subjected to dehydration under a graded series of tert-butanol. This was followed by critical point drying, gold sputtering and SEM observation (Quanta 450, Waltham, MA, United States) performed at 3.0 kV under 5000 and 50000× magnifications as described previously (Hou et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

AHLs Regulate Biofilm Formation and Swimming Motility of Hafnia alvei H4

et al. 2019

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The aim of this study was to evaluate the role of N -acyl homoserine lactones (AHLs) in the regulation of swimming motility of Hafnia alvei H4 and its biofilm formation on 96-well plate, glass and stainless-steel surfaces. The luxI gene, which codes for an enzyme involved in AHL synthesis, was deleted to generate a luxI mutant (Δ luxI ). The mutant produced no AHL, and the relative expression of the luxR gene was significantly ( P < 0.05) decreased. In addition, q RT-PCR analysis showed that the relative expression of the luxR gene in Δ luxI was stimulated by the presence of exogenous AHLs (C4-HSL, C6-HSL, and 3-o-C8-HSL) added at concentrations ranging from of 50–250 μg/ml. Among the three AHLs, C6-HSL had the strongest effect. The ability of Δ luxI to form biofilm on 96-well plate, glass and stainless-steel surfaces was significantly reduced ( P < 0.05) compared with the wild type (WT), but was increased when provided with 150 μg/ml C4-HSL, whereas C6-HSL and 3-o-C8-HSL had no effect. Scanning electron microscopy analysis of the biofilm revealed less bacteria adhering to the surface of stainless-steel and fewer filaments were found binding to the cells compared with the WT. Furthermore, Δ luxI also exhibited significant ( P < 0.05) decrease in the expression of biofilm- and swimming motility-related genes, flgA , motA and cheA , consistent with the results observed for biofilm formation and swimming motility. Taken together, the results suggested that in H. alvei H4, C4-HSL may act as an important molecular signal through regulating the ability of the cells to form biofilm, as well as through regulating the swimming motility of the cell, and this could provide a new way to control these phenotypes of H. alvei in food processing.

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Section: Methodsmentioning

confidence: 99%

AHLs Regulate Biofilm Formation and Swimming Motility of Hafnia alvei H4

et al. 2019

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“…A diazaborine‐based copolymer with quorum‐sensing inhibitory activity in a violacein assay showed swarming inhibition by about 50 % against P. aeruginosa PAO1 at a concentration of 100 μg mL −1 , whereas the MIC (minimal inhibitory concentration) was determined to be 10 times higher . At relatively high concentrations of 10–12 m m , the food additives diallyl disulfide (DADS) and methyl 2‐methyl‐3‐furyl disulfide (MMFDS) inhibited C6‐HSL production of the enterobacterium Hafnia alvei , reduced expression levels of luxI and luxR and inhibited swarming by more than 70 % …”

Section: Swarming and Bacterial Signalingmentioning

confidence: 99%

“…[57] At relatively high concentrations of 10-12 mm,t he food additives diallyl disulfide (DADS) andm ethyl 2-methyl-3-furyl disulfide (MMFDS) inhibitedC 6-HSL production of the enterobacterium Hafnia alvei,r educed expression levels of luxI and luxR and inhibited swarming by more than 70 %. [58] In S. marcescens,p roduction of its red pigmentp rodigiosin is under control of AHL-based quorum sensing. Methanolic extracts of the benthic brown alga Padina gymnospora inhibited production of this pigment and activity guided fractionation led to a-bisabolol as active compound.…”

Section: Blockingahl Receptorsmentioning

confidence: 99%

Inhibitors of Bacterial Swarming Behavior

Rütschlin

Böttcher

2019

Chemistry A European J

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Bacteria can migrate in groups of flagella‐driven cells over semisolid surfaces. This coordinated form of motility is called swarming behavior. Swarming is associated with enhanced virulence and antibiotic resistance of various human pathogens and may be considered as favorable adaptation to the diverse challenges that microbes face in rapidly changing environments. Consequently, the differentiation of motile swarmer cells is tightly regulated and involves multi‐layered signaling networks. Controlling swarming behavior is of major interest for the development of novel anti‐infective strategies. In addition, compounds that block swarming represent important tools for more detailed insights into the molecular mechanisms of the coordination of bacterial population behavior. Over the past decades, there has been major progress in the discovery of small‐molecule modulators and mechanisms that allow selective inhibition of swarming behavior. Herein, an overview of the achievements in the field and future directions and challenges will be presented.

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“…Quorum sensing (QS) is a communication system that allows bacteria to monitor their population density through the production and sensing of small signaling molecules ( 31 – 33 ). Chromobacterium violaceum is a gram-negative bacterium that synthesizes a violet pigment (violacein) as a result of QS ( 34 ).…”

Section: Introductionmentioning

confidence: 99%

“…Until now, only a few studies have developed food preservation techniques based on the anti-QS property of EOs ( 32 ). The search for an efficient QS inhibitor that can inhibit the spoilage of food products is a promising alternative ( 33 ). A stable NE with OEO that has antimicrobial and QS-inhibitory properties could be useful in food systems to avoid spoilage.…”

Section: Introductionmentioning

confidence: 99%

Rheological Behavior, Antimicrobial and Quorum Sensig Inhibition Study of an Argentinean Oregano Essential Oil Nanoemulsion

et al. 2020

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In this study, Argentinean oregano essential oil (OEO) nanoemulsions (NEs) were developed. Four NEs were prepared: a control (CNE), EONE1 (10.6 mg EO/g NE), EONE2 (106 mg EO/ g NE), and EONE3 (160 mg EO/g NE) and tested for antimicrobial activity against Staphylococcus aureus ATCC 13565, Listeria monocytogenes Scott A, Pseudomonas aeruginosa ATCC 14213, and Escherichia coli O157:H7 using a broth microdilution assay and quorum sensing inhibition in a model using Chromobacterium violaceum ATCC 12472, where the production of violacein was quantified. The chemical composition of the EO was determined by gas chromatography–mass spectrometry. The average particle size (nm) and polydispersity index were monitored over 14 days at two different storage temperatures (4 and 23°C). A rheological behavior study was carried out using a dynamic shear rheometer, and flow curves, as well as viscoelastic properties, were determined. E. coli and L. monocytogenes were the most sensitive microorganisms to EONE (MIC of 2 and 5 mg/ml for EOEN3). Sub-MICs for NE were found at lower concentrations than those for pure EO. A significant reduction in violet pigment intensity and colorless coloration ( p < 0.05) were observed at different NE concentrations concerning the control sample. The flow behavior index ( n ) decreased, and the consistency index (k) increased when the EO concentration was increased. CNE, EONE1, and EONE2 showed liquid-like behavior (G′ < G″) in the low-frequency region, whereas a solid-like behavior (G′ > G″) was observed in the high-frequency region, presenting a viscoelastic behavior, appearing as a wormlike micellar solution. For EONE3, a strong increase in both moduli was observed with increasing OEO concentration. The G′ was about one order of magnitude higher than the G″ over the whole frequency range, indicating the presence of a gel-like structure. The incorporation of EOs into an NE increased their stability, lowering the particle size, leading to a wormlike micelle with higher viscosity. Moreover, this NE had good antimicrobial activity and novel quorum-sensing inhibition activity. The results of this study indicated that Argentinean OEO NE could be used in a food system as a natural and stable antimicrobial agent.

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Effects of Sulfide Flavors on AHL‐Mediated Quorum Sensing and Biofilm Formation of Hafnia alvei

Abstract: The sulfide flavors compounds used in this paper are commonly used in food processing in China and are listed in the national standard of Chinese food additives GB2760-2014. The application of sulfide flavors in food processing can enhance aroma and prevent food spoilage.

Cited by 14 publications

References 42 publications

AHLs Regulate Biofilm Formation and Swimming Motility of Hafnia alvei H4

AHLs Regulate Biofilm Formation and Swimming Motility of Hafnia alvei H4

Inhibitors of Bacterial Swarming Behavior

Rheological Behavior, Antimicrobial and Quorum Sensig Inhibition Study of an Argentinean Oregano Essential Oil Nanoemulsion

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